The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs
Abstract Microgravity is well-known to induce Osteopenia. However, the combined effects of microgravity and radiation that commonly exist in space have not been broadly elucidated. This research investigates the combined effects on MC3T3-E1 cells and rat femurs. In MC3T3-E1 cells, simulated microgra...
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2021
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oai:doaj.org-article:67244e61898048bebd837b78d24363462021-12-02T12:11:06ZThe combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs10.1038/s41526-021-00131-12373-8065https://doaj.org/article/67244e61898048bebd837b78d24363462021-02-01T00:00:00Zhttps://doi.org/10.1038/s41526-021-00131-1https://doaj.org/toc/2373-8065Abstract Microgravity is well-known to induce Osteopenia. However, the combined effects of microgravity and radiation that commonly exist in space have not been broadly elucidated. This research investigates the combined effects on MC3T3-E1 cells and rat femurs. In MC3T3-E1 cells, simulated microgravity and X-ray radiation, alone or combination, show decreased cell activity, increased apoptosis rates by flow cytometric analysis, and decreased Runx2 and increased Caspase-3 mRNA and protein expressions. In rat femurs, simulated microgravity and X-ray radiation, alone or combination, show increased bone loss by micro-CT test and Masson staining, decreased serum BALP levels and Runx2 mRNA expressions, and increased serum CTX-1 levels and Caspase-3 mRNA expressions. The strongest effect is observed in the combined group in MC3T3-E1 cells and rat femurs. These findings suggest that the combination of microgravity and radiation exacerbates the effects of either treatment alone on MC3T3-E1 cells and rat femurs.Jingjing DongHonghui WangGaozhi LiKe WangYingjun TanLijun ZhangYixuan WangZebing HuXinsheng CaoFei ShiShu ZhangNature PortfolioarticleBiotechnologyTP248.13-248.65PhysiologyQP1-981ENnpj Microgravity, Vol 7, Iss 1, Pp 1-8 (2021) |
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Biotechnology TP248.13-248.65 Physiology QP1-981 |
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Biotechnology TP248.13-248.65 Physiology QP1-981 Jingjing Dong Honghui Wang Gaozhi Li Ke Wang Yingjun Tan Lijun Zhang Yixuan Wang Zebing Hu Xinsheng Cao Fei Shi Shu Zhang The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs |
| description |
Abstract Microgravity is well-known to induce Osteopenia. However, the combined effects of microgravity and radiation that commonly exist in space have not been broadly elucidated. This research investigates the combined effects on MC3T3-E1 cells and rat femurs. In MC3T3-E1 cells, simulated microgravity and X-ray radiation, alone or combination, show decreased cell activity, increased apoptosis rates by flow cytometric analysis, and decreased Runx2 and increased Caspase-3 mRNA and protein expressions. In rat femurs, simulated microgravity and X-ray radiation, alone or combination, show increased bone loss by micro-CT test and Masson staining, decreased serum BALP levels and Runx2 mRNA expressions, and increased serum CTX-1 levels and Caspase-3 mRNA expressions. The strongest effect is observed in the combined group in MC3T3-E1 cells and rat femurs. These findings suggest that the combination of microgravity and radiation exacerbates the effects of either treatment alone on MC3T3-E1 cells and rat femurs. |
| format |
article |
| author |
Jingjing Dong Honghui Wang Gaozhi Li Ke Wang Yingjun Tan Lijun Zhang Yixuan Wang Zebing Hu Xinsheng Cao Fei Shi Shu Zhang |
| author_facet |
Jingjing Dong Honghui Wang Gaozhi Li Ke Wang Yingjun Tan Lijun Zhang Yixuan Wang Zebing Hu Xinsheng Cao Fei Shi Shu Zhang |
| author_sort |
Jingjing Dong |
| title |
The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs |
| title_short |
The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs |
| title_full |
The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs |
| title_fullStr |
The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs |
| title_full_unstemmed |
The combined effects of simulated microgravity and X-ray radiation on MC3T3-E1 cells and rat femurs |
| title_sort |
combined effects of simulated microgravity and x-ray radiation on mc3t3-e1 cells and rat femurs |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/67244e61898048bebd837b78d2436346 |
| work_keys_str_mv |
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