Human mammospheres secrete hormone-regulated active extracellular vesicles.

Breast cancer is a leading cause of cancer-associated death worldwide. One of the most important prognostic factors for survival is the early detection of the disease. Recent studies indicate that extracellular vesicles may provide diagnostic information for cancer management. We demonstrate the sec...

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Autores principales:	Esperanza Gonzalez, Marco Piva, Eva Rodriguez-Suarez, David Gil, Felix Royo, Felix Elortza, Juan M Falcon-Perez, Maria dM Vivanco
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/672772aaa9f04547b8b52f828d1f5bd1
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spelling	oai:doaj.org-article:672772aaa9f04547b8b52f828d1f5bd12021-11-18T08:38:59ZHuman mammospheres secrete hormone-regulated active extracellular vesicles.1932-620310.1371/journal.pone.0083955https://doaj.org/article/672772aaa9f04547b8b52f828d1f5bd12014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24404144/?tool=EBIhttps://doaj.org/toc/1932-6203Breast cancer is a leading cause of cancer-associated death worldwide. One of the most important prognostic factors for survival is the early detection of the disease. Recent studies indicate that extracellular vesicles may provide diagnostic information for cancer management. We demonstrate the secretion of extracellular vesicles by primary breast epithelial cells enriched for stem/progenitor cells cultured as mammospheres, in non-adherent conditions. Using a proteomic approach we identified proteins contained in these vesicles whose expression is affected by hormonal changes in the cellular environment. In addition, we showed that these vesicles are capable of promoting changes in expression levels of genes involved in epithelial-mesenchymal transition and stem cell markers. Our findings suggest that secreted extracellular vesicles could represent potential diagnostic and/or prognostic markers for breast cancer and support a role for extracellular vesicles in cancer progression.Esperanza GonzalezMarco PivaEva Rodriguez-SuarezDavid GilFelix RoyoFelix ElortzaJuan M Falcon-PerezMaria dM VivancoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e83955 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Esperanza Gonzalez Marco Piva Eva Rodriguez-Suarez David Gil Felix Royo Felix Elortza Juan M Falcon-Perez Maria dM Vivanco Human mammospheres secrete hormone-regulated active extracellular vesicles.
description	Breast cancer is a leading cause of cancer-associated death worldwide. One of the most important prognostic factors for survival is the early detection of the disease. Recent studies indicate that extracellular vesicles may provide diagnostic information for cancer management. We demonstrate the secretion of extracellular vesicles by primary breast epithelial cells enriched for stem/progenitor cells cultured as mammospheres, in non-adherent conditions. Using a proteomic approach we identified proteins contained in these vesicles whose expression is affected by hormonal changes in the cellular environment. In addition, we showed that these vesicles are capable of promoting changes in expression levels of genes involved in epithelial-mesenchymal transition and stem cell markers. Our findings suggest that secreted extracellular vesicles could represent potential diagnostic and/or prognostic markers for breast cancer and support a role for extracellular vesicles in cancer progression.
format	article
author	Esperanza Gonzalez Marco Piva Eva Rodriguez-Suarez David Gil Felix Royo Felix Elortza Juan M Falcon-Perez Maria dM Vivanco
author_facet	Esperanza Gonzalez Marco Piva Eva Rodriguez-Suarez David Gil Felix Royo Felix Elortza Juan M Falcon-Perez Maria dM Vivanco
author_sort	Esperanza Gonzalez
title	Human mammospheres secrete hormone-regulated active extracellular vesicles.
title_short	Human mammospheres secrete hormone-regulated active extracellular vesicles.
title_full	Human mammospheres secrete hormone-regulated active extracellular vesicles.
title_fullStr	Human mammospheres secrete hormone-regulated active extracellular vesicles.
title_full_unstemmed	Human mammospheres secrete hormone-regulated active extracellular vesicles.
title_sort	human mammospheres secrete hormone-regulated active extracellular vesicles.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/672772aaa9f04547b8b52f828d1f5bd1
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Human mammospheres secrete hormone-regulated active extracellular vesicles.

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