Transcriptional Differences in Lipid-Metabolizing Enzymes in Murine Sebocytes Derived from Sebaceous Glands of the Skin and Preputial Glands

Sebaceous glands are adnexal structures, which critically contribute to skin homeostasis and the establishment of a functional epidermal barrier. Sebocytes, the main cell population found within the sebaceous glands, are highly specialized lipid-producing cells. Sebaceous gland-resembling tissue str...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Katharina Klas, Dragan Copic, Martin Direder, Maria Laggner, Patricia Sandee Prucksamas, Florian Gruber, Hendrik Jan Ankersmit, Michael Mildner
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	skin sebaceous gland preputial gland scRNA-sequencing lipid sebocyte differentiation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/673bca0d1a06473fa520e2b758d01f59
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:673bca0d1a06473fa520e2b758d01f59
record_format	dspace
spelling	oai:doaj.org-article:673bca0d1a06473fa520e2b758d01f592021-11-11T17:06:27ZTranscriptional Differences in Lipid-Metabolizing Enzymes in Murine Sebocytes Derived from Sebaceous Glands of the Skin and Preputial Glands10.3390/ijms2221116311422-00671661-6596https://doaj.org/article/673bca0d1a06473fa520e2b758d01f592021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11631https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Sebaceous glands are adnexal structures, which critically contribute to skin homeostasis and the establishment of a functional epidermal barrier. Sebocytes, the main cell population found within the sebaceous glands, are highly specialized lipid-producing cells. Sebaceous gland-resembling tissue structures are also found in male rodents in the form of preputial glands. Similar to sebaceous glands, they are composed of lipid-specialized sebocytes. Due to a lack of adequate organ culture models for skin sebaceous glands and the fact that preputial glands are much larger and easier to handle, previous studies used preputial glands as a model for skin sebaceous glands. Here, we compared both types of sebocytes, using a single-cell RNA sequencing approach, to unravel potential similarities and differences between the two sebocyte populations. In spite of common gene expression patterns due to general lipid-producing properties, we found significant differences in the expression levels of genes encoding enzymes involved in the biogenesis of specialized lipid classes. Specifically, genes critically involved in the mevalonate pathway, including squalene synthase, as well as the sphingolipid salvage pathway, such as ceramide synthase, (acid) sphingomyelinase or acid and alkaline ceramidases, were significantly less expressed by preputial gland sebocytes. Together, our data revealed tissue-specific sebocyte populations, indicating major developmental, functional as well as biosynthetic differences between both glands. The use of preputial glands as a surrogate model to study skin sebaceous glands is therefore limited, and major differences between both glands need to be carefully considered before planning an experiment.Katharina KlasDragan CopicMartin DirederMaria LaggnerPatricia Sandee PrucksamasFlorian GruberHendrik Jan AnkersmitMichael MildnerMDPI AGarticleskinsebaceous glandpreputial glandscRNA-sequencinglipidsebocyte differentiationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11631, p 11631 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	skin sebaceous gland preputial gland scRNA-sequencing lipid sebocyte differentiation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	skin sebaceous gland preputial gland scRNA-sequencing lipid sebocyte differentiation Biology (General) QH301-705.5 Chemistry QD1-999 Katharina Klas Dragan Copic Martin Direder Maria Laggner Patricia Sandee Prucksamas Florian Gruber Hendrik Jan Ankersmit Michael Mildner Transcriptional Differences in Lipid-Metabolizing Enzymes in Murine Sebocytes Derived from Sebaceous Glands of the Skin and Preputial Glands
description	Sebaceous glands are adnexal structures, which critically contribute to skin homeostasis and the establishment of a functional epidermal barrier. Sebocytes, the main cell population found within the sebaceous glands, are highly specialized lipid-producing cells. Sebaceous gland-resembling tissue structures are also found in male rodents in the form of preputial glands. Similar to sebaceous glands, they are composed of lipid-specialized sebocytes. Due to a lack of adequate organ culture models for skin sebaceous glands and the fact that preputial glands are much larger and easier to handle, previous studies used preputial glands as a model for skin sebaceous glands. Here, we compared both types of sebocytes, using a single-cell RNA sequencing approach, to unravel potential similarities and differences between the two sebocyte populations. In spite of common gene expression patterns due to general lipid-producing properties, we found significant differences in the expression levels of genes encoding enzymes involved in the biogenesis of specialized lipid classes. Specifically, genes critically involved in the mevalonate pathway, including squalene synthase, as well as the sphingolipid salvage pathway, such as ceramide synthase, (acid) sphingomyelinase or acid and alkaline ceramidases, were significantly less expressed by preputial gland sebocytes. Together, our data revealed tissue-specific sebocyte populations, indicating major developmental, functional as well as biosynthetic differences between both glands. The use of preputial glands as a surrogate model to study skin sebaceous glands is therefore limited, and major differences between both glands need to be carefully considered before planning an experiment.
format	article
author	Katharina Klas Dragan Copic Martin Direder Maria Laggner Patricia Sandee Prucksamas Florian Gruber Hendrik Jan Ankersmit Michael Mildner
author_facet	Katharina Klas Dragan Copic Martin Direder Maria Laggner Patricia Sandee Prucksamas Florian Gruber Hendrik Jan Ankersmit Michael Mildner
author_sort	Katharina Klas
title	Transcriptional Differences in Lipid-Metabolizing Enzymes in Murine Sebocytes Derived from Sebaceous Glands of the Skin and Preputial Glands
title_short	Transcriptional Differences in Lipid-Metabolizing Enzymes in Murine Sebocytes Derived from Sebaceous Glands of the Skin and Preputial Glands
title_full	Transcriptional Differences in Lipid-Metabolizing Enzymes in Murine Sebocytes Derived from Sebaceous Glands of the Skin and Preputial Glands
title_fullStr	Transcriptional Differences in Lipid-Metabolizing Enzymes in Murine Sebocytes Derived from Sebaceous Glands of the Skin and Preputial Glands
title_full_unstemmed	Transcriptional Differences in Lipid-Metabolizing Enzymes in Murine Sebocytes Derived from Sebaceous Glands of the Skin and Preputial Glands
title_sort	transcriptional differences in lipid-metabolizing enzymes in murine sebocytes derived from sebaceous glands of the skin and preputial glands
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/673bca0d1a06473fa520e2b758d01f59
work_keys_str_mv	AT katharinaklas transcriptionaldifferencesinlipidmetabolizingenzymesinmurinesebocytesderivedfromsebaceousglandsoftheskinandpreputialglands AT dragancopic transcriptionaldifferencesinlipidmetabolizingenzymesinmurinesebocytesderivedfromsebaceousglandsoftheskinandpreputialglands AT martindireder transcriptionaldifferencesinlipidmetabolizingenzymesinmurinesebocytesderivedfromsebaceousglandsoftheskinandpreputialglands AT marialaggner transcriptionaldifferencesinlipidmetabolizingenzymesinmurinesebocytesderivedfromsebaceousglandsoftheskinandpreputialglands AT patriciasandeeprucksamas transcriptionaldifferencesinlipidmetabolizingenzymesinmurinesebocytesderivedfromsebaceousglandsoftheskinandpreputialglands AT floriangruber transcriptionaldifferencesinlipidmetabolizingenzymesinmurinesebocytesderivedfromsebaceousglandsoftheskinandpreputialglands AT hendrikjanankersmit transcriptionaldifferencesinlipidmetabolizingenzymesinmurinesebocytesderivedfromsebaceousglandsoftheskinandpreputialglands AT michaelmildner transcriptionaldifferencesinlipidmetabolizingenzymesinmurinesebocytesderivedfromsebaceousglandsoftheskinandpreputialglands
_version_	1718432181126168576

Transcriptional Differences in Lipid-Metabolizing Enzymes in Murine Sebocytes Derived from Sebaceous Glands of the Skin and Preputial Glands

Ejemplares similares