SELECTED CELLULAR IMMUNITY INDICATORS IN DIAGNOSTICS OF ACUTE MESENTERIAL ISCHEMIA

Background. The problem of early diagnosis of acute mesenteric ischemia is still relevant today. In this connection, it is of interest to search for a diagnostic marker of this pathological condition. Up to 80 % of all immunocompetent cells are localized in the intestinal mucosa. So, we can assume t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Z. A. Artamonova, E. V. Namokonov
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2017
Materias:	mesenteric ischemia cellular immunity diagnostics experiment Science Q
Acceso en línea:	https://doaj.org/article/673c44d8d03b4e03ae7a6c35210bf1bd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:673c44d8d03b4e03ae7a6c35210bf1bd
record_format	dspace
spelling	oai:doaj.org-article:673c44d8d03b4e03ae7a6c35210bf1bd2021-11-23T06:14:39ZSELECTED CELLULAR IMMUNITY INDICATORS IN DIAGNOSTICS OF ACUTE MESENTERIAL ISCHEMIA2541-94202587-959610.12737/article_5a0a884a825708.48688160https://doaj.org/article/673c44d8d03b4e03ae7a6c35210bf1bd2017-11-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/475https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. The problem of early diagnosis of acute mesenteric ischemia is still relevant today. In this connection, it is of interest to search for a diagnostic marker of this pathological condition. Up to 80 % of all immunocompetent cells are localized in the intestinal mucosa. So, we can assume that the mesenteric channel occlusion can result in changes in the subpopulation structure of lymphocytes. The aim of the study is to analyse some parameters of the subpopulation structure of lymphocytes at various stages of acute mesenteric ischemia in the experiment. Materials and methods. The experiment was performed on 32 adult male rats - with an average mass of 200 g. The animals were divided into 4 equal groups. The first group was a control group. All other animals underwent laparotomy, blood sampling from the posterior vena cava, ligation of the mesenteric artery at the base of the mesentery root, suturing the wound. After 3, 6 and 8 hours, blood sampling, small bowel biopsy were performed in the second, third and fourth groups. The subpopulation structure of lymphocytes was assessed by direct immunofluorescence staining. Morphological material was stained with hematoxylin-eosin. Results. The complete occlusion of the cranial mesenteric artery was revealed to be accompanied by a decrease in the lymphocytes level, an increase in CD4/CD8 ratio. Conclusion. The data obtained were to develop a diagnostic method for acute mesenteric ischemia (Patent N 2552338, Russian Federation).Z. A. ArtamonovaE. V. NamokonovScientific Сentre for Family Health and Human Reproduction Problemsarticlemesenteric ischemiacellular immunitydiagnosticsexperimentScienceQRUActa Biomedica Scientifica, Vol 2, Iss 6, Pp 92-95 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	mesenteric ischemia cellular immunity diagnostics experiment Science Q
spellingShingle	mesenteric ischemia cellular immunity diagnostics experiment Science Q Z. A. Artamonova E. V. Namokonov SELECTED CELLULAR IMMUNITY INDICATORS IN DIAGNOSTICS OF ACUTE MESENTERIAL ISCHEMIA
description	Background. The problem of early diagnosis of acute mesenteric ischemia is still relevant today. In this connection, it is of interest to search for a diagnostic marker of this pathological condition. Up to 80 % of all immunocompetent cells are localized in the intestinal mucosa. So, we can assume that the mesenteric channel occlusion can result in changes in the subpopulation structure of lymphocytes. The aim of the study is to analyse some parameters of the subpopulation structure of lymphocytes at various stages of acute mesenteric ischemia in the experiment. Materials and methods. The experiment was performed on 32 adult male rats - with an average mass of 200 g. The animals were divided into 4 equal groups. The first group was a control group. All other animals underwent laparotomy, blood sampling from the posterior vena cava, ligation of the mesenteric artery at the base of the mesentery root, suturing the wound. After 3, 6 and 8 hours, blood sampling, small bowel biopsy were performed in the second, third and fourth groups. The subpopulation structure of lymphocytes was assessed by direct immunofluorescence staining. Morphological material was stained with hematoxylin-eosin. Results. The complete occlusion of the cranial mesenteric artery was revealed to be accompanied by a decrease in the lymphocytes level, an increase in CD4/CD8 ratio. Conclusion. The data obtained were to develop a diagnostic method for acute mesenteric ischemia (Patent N 2552338, Russian Federation).
format	article
author	Z. A. Artamonova E. V. Namokonov
author_facet	Z. A. Artamonova E. V. Namokonov
author_sort	Z. A. Artamonova
title	SELECTED CELLULAR IMMUNITY INDICATORS IN DIAGNOSTICS OF ACUTE MESENTERIAL ISCHEMIA
title_short	SELECTED CELLULAR IMMUNITY INDICATORS IN DIAGNOSTICS OF ACUTE MESENTERIAL ISCHEMIA
title_full	SELECTED CELLULAR IMMUNITY INDICATORS IN DIAGNOSTICS OF ACUTE MESENTERIAL ISCHEMIA
title_fullStr	SELECTED CELLULAR IMMUNITY INDICATORS IN DIAGNOSTICS OF ACUTE MESENTERIAL ISCHEMIA
title_full_unstemmed	SELECTED CELLULAR IMMUNITY INDICATORS IN DIAGNOSTICS OF ACUTE MESENTERIAL ISCHEMIA
title_sort	selected cellular immunity indicators in diagnostics of acute mesenterial ischemia
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2017
url	https://doaj.org/article/673c44d8d03b4e03ae7a6c35210bf1bd
work_keys_str_mv	AT zaartamonova selectedcellularimmunityindicatorsindiagnosticsofacutemesenterialischemia AT evnamokonov selectedcellularimmunityindicatorsindiagnosticsofacutemesenterialischemia
_version_	1718417011266027520

SELECTED CELLULAR IMMUNITY INDICATORS IN DIAGNOSTICS OF ACUTE MESENTERIAL ISCHEMIA

Ejemplares similares