Neuroprotective Effects and Related Mechanisms of Echinacoside in MPTP-Induced PD Mice

Zhen-Nian Zhang,1,* Zhen Hui,1,* Chang Chen,1 Yan Liang,1 Li-Li Tang,1 Su-Lei Wang,1 Cheng-Cheng Xu,1 Hui Yang,1 Jing-Si Zhang,2 Yang Zhao1 1Department of Neurology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210022, People’s Republi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang ZN, Hui Z, Chen C, Liang Y, Tang LL, Wang SL, Xu CC, Yang H, Zhang JS, Zhao Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	parkinson's disease mptp echinacoside α-synuclein oxidative stress neuroprotection bdnf gdnf il-6 tnf-α il-10 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/673d7102216a44db90ef09a8f10a059f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:673d7102216a44db90ef09a8f10a059f
record_format	dspace
spelling	oai:doaj.org-article:673d7102216a44db90ef09a8f10a059f2021-12-02T17:21:57ZNeuroprotective Effects and Related Mechanisms of Echinacoside in MPTP-Induced PD Mice1178-2021https://doaj.org/article/673d7102216a44db90ef09a8f10a059f2021-06-01T00:00:00Zhttps://www.dovepress.com/neuroprotective-effects-and-related-mechanisms-of-echinacoside-in-mptp-peer-reviewed-fulltext-article-NDThttps://doaj.org/toc/1178-2021Zhen-Nian Zhang,1,* Zhen Hui,1,* Chang Chen,1 Yan Liang,1 Li-Li Tang,1 Su-Lei Wang,1 Cheng-Cheng Xu,1 Hui Yang,1 Jing-Si Zhang,2 Yang Zhao1 1Department of Neurology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210022, People’s Republic of China; 2Department of Neurology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yang ZhaoDepartment of Neurology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, No. 157 of Daming Road, Qinhuai District, Nanjing, 210022, People’s Republic of ChinaTel/Fax +86 13851498418Email zhao_dr66@163.comObjective: To explore the neuroprotective effect and the related mechanisms of echinacoside (ECH) in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson’s disease (PD) mice.Methods: Parkinson’s disease is induced in mice by MPTP and the neurobehaviors of mice in different groups are observed. Then, immunohistochemistry and Western blot analysis are adopted to measure the expression of tyrosine hydroxylase (TH) and α-synuclein in the substantia nigra (SN). The content of dopamine (DA) and other neurotransmitters in the brain is detected by high-performance liquid chromatography. The expression of nerve growth factors and inflammatory factors in SN in mice in each group is measured by quantitative polymerase chain reaction. Finally, the expression of oxidative stress-related parameters in each group is measured.Results: Compared with the model group, the pole-climbing time among mice in the moderate and high-dose ECH groups is significantly reduced (P < 0.01). The rotarod staying time, as well as fore and hind-limb strides, shows a significant increase (P < 0.01), as does spontaneous activity (P < 0.01). Moreover, the expression levels of TH, DA, glial cell line-derived neurotrophic factor, and brain-derived neurotrophic factor in SN in mice show significant increases in these two groups (P < 0.01). The content of superoxide dismutase, catalase, and glutathione peroxidase indicates significant increases in the low, moderate, and high-dose ECH groups (P < 0.01), and the content of MDA was reduced (P < 0.01). In the high-dose ECH group, the expression of interleukin (IL) 6 and tumor necrosis factor-α is significantly reduced (P < 0.01), while the expression of IL-10 shows a marked increase (P < 0.01) alongside a decrease in the expression of α-synuclein (P < 0.01).Conclusion: Echinacoside improves neurobehavioral symptoms in PD mice and significantly increases the expression of TH and DA. The neuroprotective effect potentially correlates with anti-inflammation and anti-oxidation actions, promotes the expression of nerve growth factor, and reduces the accumulation of α-synuclein.Keywords: Parkinson’s disease, MPTP, echinacoside, α-synuclein, oxidative stress, neuroprotection, BDNF, GDNF, IL-6, TNF-α, IL-10Zhang ZNHui ZChen CLiang YTang LLWang SLXu CCYang HZhang JSZhao YDove Medical Pressarticleparkinson's diseasemptpechinacosideα-synucleinoxidative stressneuroprotectionbdnfgdnfil-6tnf-αil-10Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 17, Pp 1779-1792 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	parkinson's disease mptp echinacoside α-synuclein oxidative stress neuroprotection bdnf gdnf il-6 tnf-α il-10 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	parkinson's disease mptp echinacoside α-synuclein oxidative stress neuroprotection bdnf gdnf il-6 tnf-α il-10 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Zhang ZN Hui Z Chen C Liang Y Tang LL Wang SL Xu CC Yang H Zhang JS Zhao Y Neuroprotective Effects and Related Mechanisms of Echinacoside in MPTP-Induced PD Mice
description	Zhen-Nian Zhang,1,* Zhen Hui,1,* Chang Chen,1 Yan Liang,1 Li-Li Tang,1 Su-Lei Wang,1 Cheng-Cheng Xu,1 Hui Yang,1 Jing-Si Zhang,2 Yang Zhao1 1Department of Neurology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210022, People’s Republic of China; 2Department of Neurology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yang ZhaoDepartment of Neurology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, No. 157 of Daming Road, Qinhuai District, Nanjing, 210022, People’s Republic of ChinaTel/Fax +86 13851498418Email zhao_dr66@163.comObjective: To explore the neuroprotective effect and the related mechanisms of echinacoside (ECH) in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson’s disease (PD) mice.Methods: Parkinson’s disease is induced in mice by MPTP and the neurobehaviors of mice in different groups are observed. Then, immunohistochemistry and Western blot analysis are adopted to measure the expression of tyrosine hydroxylase (TH) and α-synuclein in the substantia nigra (SN). The content of dopamine (DA) and other neurotransmitters in the brain is detected by high-performance liquid chromatography. The expression of nerve growth factors and inflammatory factors in SN in mice in each group is measured by quantitative polymerase chain reaction. Finally, the expression of oxidative stress-related parameters in each group is measured.Results: Compared with the model group, the pole-climbing time among mice in the moderate and high-dose ECH groups is significantly reduced (P < 0.01). The rotarod staying time, as well as fore and hind-limb strides, shows a significant increase (P < 0.01), as does spontaneous activity (P < 0.01). Moreover, the expression levels of TH, DA, glial cell line-derived neurotrophic factor, and brain-derived neurotrophic factor in SN in mice show significant increases in these two groups (P < 0.01). The content of superoxide dismutase, catalase, and glutathione peroxidase indicates significant increases in the low, moderate, and high-dose ECH groups (P < 0.01), and the content of MDA was reduced (P < 0.01). In the high-dose ECH group, the expression of interleukin (IL) 6 and tumor necrosis factor-α is significantly reduced (P < 0.01), while the expression of IL-10 shows a marked increase (P < 0.01) alongside a decrease in the expression of α-synuclein (P < 0.01).Conclusion: Echinacoside improves neurobehavioral symptoms in PD mice and significantly increases the expression of TH and DA. The neuroprotective effect potentially correlates with anti-inflammation and anti-oxidation actions, promotes the expression of nerve growth factor, and reduces the accumulation of α-synuclein.Keywords: Parkinson’s disease, MPTP, echinacoside, α-synuclein, oxidative stress, neuroprotection, BDNF, GDNF, IL-6, TNF-α, IL-10
format	article
author	Zhang ZN Hui Z Chen C Liang Y Tang LL Wang SL Xu CC Yang H Zhang JS Zhao Y
author_facet	Zhang ZN Hui Z Chen C Liang Y Tang LL Wang SL Xu CC Yang H Zhang JS Zhao Y
author_sort	Zhang ZN
title	Neuroprotective Effects and Related Mechanisms of Echinacoside in MPTP-Induced PD Mice
title_short	Neuroprotective Effects and Related Mechanisms of Echinacoside in MPTP-Induced PD Mice
title_full	Neuroprotective Effects and Related Mechanisms of Echinacoside in MPTP-Induced PD Mice
title_fullStr	Neuroprotective Effects and Related Mechanisms of Echinacoside in MPTP-Induced PD Mice
title_full_unstemmed	Neuroprotective Effects and Related Mechanisms of Echinacoside in MPTP-Induced PD Mice
title_sort	neuroprotective effects and related mechanisms of echinacoside in mptp-induced pd mice
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/673d7102216a44db90ef09a8f10a059f
work_keys_str_mv	AT zhangzn neuroprotectiveeffectsandrelatedmechanismsofechinacosideinmptpinducedpdmice AT huiz neuroprotectiveeffectsandrelatedmechanismsofechinacosideinmptpinducedpdmice AT chenc neuroprotectiveeffectsandrelatedmechanismsofechinacosideinmptpinducedpdmice AT liangy neuroprotectiveeffectsandrelatedmechanismsofechinacosideinmptpinducedpdmice AT tangll neuroprotectiveeffectsandrelatedmechanismsofechinacosideinmptpinducedpdmice AT wangsl neuroprotectiveeffectsandrelatedmechanismsofechinacosideinmptpinducedpdmice AT xucc neuroprotectiveeffectsandrelatedmechanismsofechinacosideinmptpinducedpdmice AT yangh neuroprotectiveeffectsandrelatedmechanismsofechinacosideinmptpinducedpdmice AT zhangjs neuroprotectiveeffectsandrelatedmechanismsofechinacosideinmptpinducedpdmice AT zhaoy neuroprotectiveeffectsandrelatedmechanismsofechinacosideinmptpinducedpdmice
_version_	1718381029973032960

Neuroprotective Effects and Related Mechanisms of Echinacoside in MPTP-Induced PD Mice

Ejemplares similares