LYMPHOCYTE APOPTOSIS IN PSORIASIS

Abstract. Forty-two patients with progressive vulgar psoriasis (PASI = 19.7 ± 1.5) and 40 healthy volunteers were under investigation. Psoriatic patients were characterized by increased number of CD4+ CD95+ peripheral blood T lymphocytes, which correlates with clinical psoriatic score, and by increa...

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Autores principales:	О. M. Kapuler, Е. V. Nelyubin, D. A. Kaut, S. V. Sibiryak
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	spontaneous lymphocyte apoptosis induced lymphocyte apoptosis soluble fas receptor psoriasis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/673f94dce42a41af8aea2bd13aab9667
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spelling	oai:doaj.org-article:673f94dce42a41af8aea2bd13aab96672021-11-18T08:03:42ZLYMPHOCYTE APOPTOSIS IN PSORIASIS1563-06252313-741X10.15789/1563-0625-2006-4-531-538https://doaj.org/article/673f94dce42a41af8aea2bd13aab96672014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/485https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Forty-two patients with progressive vulgar psoriasis (PASI = 19.7 ± 1.5) and 40 healthy volunteers were under investigation. Psoriatic patients were characterized by increased number of CD4+ CD95+ peripheral blood T lymphocytes, which correlates with clinical psoriatic score, and by increased levels of soluble Fas (sFas) in serum, as compared to controls (resp., 1868.1 ± 186.8 pg/ml vs. 1281.4 ± 142.5 pg/ml, PLSD = 0.019). The levels of spontaneous lymphocyte apoptosis and anti-Fas (Mab)-induced apoptosis in psoriatic patients did not differ from the controls. However, apoptosis induced by “oxidative stress” (50 M Н202, 4 hrs) was depressed in the patients. Moreover, a simultaneous assessment of cell cycle structure (metachromatic staining with Acridine Orange), apoptosis and Fas receptor expression (AnnV-FITC/antiFas mAbs-PE staining) following a short-term mitogenic stimulation (PHA-P, 5 µg/ml, 24 hrs) were performed. We found no marked differences in mitogenic reactivity, activation-induced apoptosis, and activation-induced Fas receptor expression when studying lymphocytes from healthy donors and psoriatic patients. However, PHA-activated lymphocytes from psoriatic patients displayed a significantly decreased ratio of AnnV+CD95+ to the total AnnV+ subpopulation, thus suggesting a decreased role of Fas-dependent mechanisms of apoptosis during the cell activation. The data obtained confirm a view, that an abnormal lymphocyte “apoptotic reactivity”, which plays a crucial role in the mechanisms of autoimmunity, may also of importance in the pathogenesis of psoriasis.О. M. KapulerЕ. V. NelyubinD. A. KautS. V. SibiryakSPb RAACIarticlespontaneous lymphocyte apoptosisinduced lymphocyte apoptosissoluble fas receptorpsoriasisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 8, Iss 4, Pp 531-538 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	spontaneous lymphocyte apoptosis induced lymphocyte apoptosis soluble fas receptor psoriasis Immunologic diseases. Allergy RC581-607
spellingShingle	spontaneous lymphocyte apoptosis induced lymphocyte apoptosis soluble fas receptor psoriasis Immunologic diseases. Allergy RC581-607 О. M. Kapuler Е. V. Nelyubin D. A. Kaut S. V. Sibiryak LYMPHOCYTE APOPTOSIS IN PSORIASIS
description	Abstract. Forty-two patients with progressive vulgar psoriasis (PASI = 19.7 ± 1.5) and 40 healthy volunteers were under investigation. Psoriatic patients were characterized by increased number of CD4+ CD95+ peripheral blood T lymphocytes, which correlates with clinical psoriatic score, and by increased levels of soluble Fas (sFas) in serum, as compared to controls (resp., 1868.1 ± 186.8 pg/ml vs. 1281.4 ± 142.5 pg/ml, PLSD = 0.019). The levels of spontaneous lymphocyte apoptosis and anti-Fas (Mab)-induced apoptosis in psoriatic patients did not differ from the controls. However, apoptosis induced by “oxidative stress” (50 M Н202, 4 hrs) was depressed in the patients. Moreover, a simultaneous assessment of cell cycle structure (metachromatic staining with Acridine Orange), apoptosis and Fas receptor expression (AnnV-FITC/antiFas mAbs-PE staining) following a short-term mitogenic stimulation (PHA-P, 5 µg/ml, 24 hrs) were performed. We found no marked differences in mitogenic reactivity, activation-induced apoptosis, and activation-induced Fas receptor expression when studying lymphocytes from healthy donors and psoriatic patients. However, PHA-activated lymphocytes from psoriatic patients displayed a significantly decreased ratio of AnnV+CD95+ to the total AnnV+ subpopulation, thus suggesting a decreased role of Fas-dependent mechanisms of apoptosis during the cell activation. The data obtained confirm a view, that an abnormal lymphocyte “apoptotic reactivity”, which plays a crucial role in the mechanisms of autoimmunity, may also of importance in the pathogenesis of psoriasis.
format	article
author	О. M. Kapuler Е. V. Nelyubin D. A. Kaut S. V. Sibiryak
author_facet	О. M. Kapuler Е. V. Nelyubin D. A. Kaut S. V. Sibiryak
author_sort	О. M. Kapuler
title	LYMPHOCYTE APOPTOSIS IN PSORIASIS
title_short	LYMPHOCYTE APOPTOSIS IN PSORIASIS
title_full	LYMPHOCYTE APOPTOSIS IN PSORIASIS
title_fullStr	LYMPHOCYTE APOPTOSIS IN PSORIASIS
title_full_unstemmed	LYMPHOCYTE APOPTOSIS IN PSORIASIS
title_sort	lymphocyte apoptosis in psoriasis
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/673f94dce42a41af8aea2bd13aab9667
work_keys_str_mv	AT omkapuler lymphocyteapoptosisinpsoriasis AT evnelyubin lymphocyteapoptosisinpsoriasis AT dakaut lymphocyteapoptosisinpsoriasis AT svsibiryak lymphocyteapoptosisinpsoriasis
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LYMPHOCYTE APOPTOSIS IN PSORIASIS

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