Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma

Abstract Medulloblastoma (MB) is the most common type of brain malignancy in children. Molecular profiling has become an important component to select patients for therapeutic approaches, allowing for personalized therapy. In this study, we successfully identified detectable levels of tumor-derived...

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Autores principales:	Yanling Sun, Miao Li, Siqi Ren, Yan Liu, Jin Zhang, Shuting Li, Wenchao Gao, Xiaojun Gong, Jingjing Liu, Yuan Wang, Shuxu Du, Liming Sun, Wanshui Wu, Yongji Tian
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/67450cab29a44b85b5b48b10b84d70e3
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spelling	oai:doaj.org-article:67450cab29a44b85b5b48b10b84d70e32021-12-02T13:34:31ZExploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma10.1038/s41598-021-85178-62045-2322https://doaj.org/article/67450cab29a44b85b5b48b10b84d70e32021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85178-6https://doaj.org/toc/2045-2322Abstract Medulloblastoma (MB) is the most common type of brain malignancy in children. Molecular profiling has become an important component to select patients for therapeutic approaches, allowing for personalized therapy. In this study, we successfully identified detectable levels of tumor-derived cell-free DNA (cfDNA) in cerebrospinal fluid (CSF) samples of patients with MB. Furthermore, cfDNA from CSF can interrogate for tumor-associated molecular clues. MB-associated alterations from CSF, tumor, and post-chemotherapy plasma were compared by deep sequencing on next-generation sequencing platform. Shared alterations exist between CSF and matched tumor tissues. More alternations were detected in circulating tumor DNA from CSF than those in genomic DNA from primary tumor. It was feasible to detect MB-associated mutations in plasma of patients treated with chemotherapy. Collectively, CSF supernatant can be used to monitor genomic alterations, as a superior technique as long as tumor-derived cfDNA can be isolated from CSF successfully.Yanling SunMiao LiSiqi RenYan LiuJin ZhangShuting LiWenchao GaoXiaojun GongJingjing LiuYuan WangShuxu DuLiming SunWanshui WuYongji TianNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yanling Sun Miao Li Siqi Ren Yan Liu Jin Zhang Shuting Li Wenchao Gao Xiaojun Gong Jingjing Liu Yuan Wang Shuxu Du Liming Sun Wanshui Wu Yongji Tian Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma
description	Abstract Medulloblastoma (MB) is the most common type of brain malignancy in children. Molecular profiling has become an important component to select patients for therapeutic approaches, allowing for personalized therapy. In this study, we successfully identified detectable levels of tumor-derived cell-free DNA (cfDNA) in cerebrospinal fluid (CSF) samples of patients with MB. Furthermore, cfDNA from CSF can interrogate for tumor-associated molecular clues. MB-associated alterations from CSF, tumor, and post-chemotherapy plasma were compared by deep sequencing on next-generation sequencing platform. Shared alterations exist between CSF and matched tumor tissues. More alternations were detected in circulating tumor DNA from CSF than those in genomic DNA from primary tumor. It was feasible to detect MB-associated mutations in plasma of patients treated with chemotherapy. Collectively, CSF supernatant can be used to monitor genomic alterations, as a superior technique as long as tumor-derived cfDNA can be isolated from CSF successfully.
format	article
author	Yanling Sun Miao Li Siqi Ren Yan Liu Jin Zhang Shuting Li Wenchao Gao Xiaojun Gong Jingjing Liu Yuan Wang Shuxu Du Liming Sun Wanshui Wu Yongji Tian
author_facet	Yanling Sun Miao Li Siqi Ren Yan Liu Jin Zhang Shuting Li Wenchao Gao Xiaojun Gong Jingjing Liu Yuan Wang Shuxu Du Liming Sun Wanshui Wu Yongji Tian
author_sort	Yanling Sun
title	Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma
title_short	Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma
title_full	Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma
title_fullStr	Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma
title_full_unstemmed	Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma
title_sort	exploring genetic alterations in circulating tumor dna from cerebrospinal fluid of pediatric medulloblastoma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/67450cab29a44b85b5b48b10b84d70e3
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Exploring genetic alterations in circulating tumor DNA from cerebrospinal fluid of pediatric medulloblastoma

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