Transcriptome analysis of upland cotton revealed novel pathways to scavenge reactive oxygen species (ROS) responding to Na2SO4 tolerance

Abstract Salinity is an extensive and adverse environmental stress to crop plants across the globe, and a major abiotic constraint responsible for limited crop production threatening the crop security. Soil salinization is a widespread problem across the globe, threatening the crop production and fo...

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Autores principales:	Qinqin Wang, Xuke Lu, Xiugui Chen, Waqar Afzal Malik, Delong Wang, Lanjie Zhao, Junjuan Wang, Shuai Wang, Lixue Guo, Ruifeng Cui, Mingge Han, Cun Rui, Yuexin Zhang, Yapeng Fan, Chao Chen, Wuwei Ye
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/67453cc4284841bbb9df332d0750a02f
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spelling	oai:doaj.org-article:67453cc4284841bbb9df332d0750a02f2021-12-02T13:39:55ZTranscriptome analysis of upland cotton revealed novel pathways to scavenge reactive oxygen species (ROS) responding to Na2SO4 tolerance10.1038/s41598-021-87999-x2045-2322https://doaj.org/article/67453cc4284841bbb9df332d0750a02f2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87999-xhttps://doaj.org/toc/2045-2322Abstract Salinity is an extensive and adverse environmental stress to crop plants across the globe, and a major abiotic constraint responsible for limited crop production threatening the crop security. Soil salinization is a widespread problem across the globe, threatening the crop production and food security. Salinity impairs plant growth and development via reduction in osmotic potential, cytotoxicity due to excessive uptake of ions such as sodium (Na+) and chloride (Cl−), and nutritional imbalance. Cotton, being the most cultivated crop on saline-alkaline soils, it is of great importance to elucidate the mechanisms involved in Na2SO4 tolerance which is still lacking in upland cotton. Zhong 9835, a Na2SO4 resistant cultivar was screened for transcriptomic studies through various levels of Na2SO4 treatments, which results into identification of 3329 differentially expressed genes (DEGs) in roots, stems and leave at 300 mM Na2SO4 stress till 12 h in compared to control. According to gene functional annotation analysis, genes involved in reactive oxygen species (ROS) scavenging system including osmotic stress and ion toxicity were significantly up-regulated, especially GST (glutathione transferase). In addition, analysis for sulfur metabolism, results in to identification of two rate limiting enzymes [APR (Gh_D05G1637) and OASTL (Gh_A13G0863)] during synthesis of GSH from SO4 2−. Furthermore, we also observed a crosstalk of the hormones and TFs (transcription factors) enriched in hormone signal transduction pathway. Genes related to IAA exceeds the rest of hormones followed by ubiquitin related genes which are greater than TFs. The analysis of the expression profiles of diverse tissues under Na2SO4 stress and identification of relevant key hub genes in a network crosstalk will provide a strong foundation and valuable clues for genetic improvements of cotton in response to various salt stresses.Qinqin WangXuke LuXiugui ChenWaqar Afzal MalikDelong WangLanjie ZhaoJunjuan WangShuai WangLixue GuoRuifeng CuiMingge HanCun RuiYuexin ZhangYapeng FanChao ChenWuwei YeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qinqin Wang Xuke Lu Xiugui Chen Waqar Afzal Malik Delong Wang Lanjie Zhao Junjuan Wang Shuai Wang Lixue Guo Ruifeng Cui Mingge Han Cun Rui Yuexin Zhang Yapeng Fan Chao Chen Wuwei Ye Transcriptome analysis of upland cotton revealed novel pathways to scavenge reactive oxygen species (ROS) responding to Na2SO4 tolerance
description	Abstract Salinity is an extensive and adverse environmental stress to crop plants across the globe, and a major abiotic constraint responsible for limited crop production threatening the crop security. Soil salinization is a widespread problem across the globe, threatening the crop production and food security. Salinity impairs plant growth and development via reduction in osmotic potential, cytotoxicity due to excessive uptake of ions such as sodium (Na+) and chloride (Cl−), and nutritional imbalance. Cotton, being the most cultivated crop on saline-alkaline soils, it is of great importance to elucidate the mechanisms involved in Na2SO4 tolerance which is still lacking in upland cotton. Zhong 9835, a Na2SO4 resistant cultivar was screened for transcriptomic studies through various levels of Na2SO4 treatments, which results into identification of 3329 differentially expressed genes (DEGs) in roots, stems and leave at 300 mM Na2SO4 stress till 12 h in compared to control. According to gene functional annotation analysis, genes involved in reactive oxygen species (ROS) scavenging system including osmotic stress and ion toxicity were significantly up-regulated, especially GST (glutathione transferase). In addition, analysis for sulfur metabolism, results in to identification of two rate limiting enzymes [APR (Gh_D05G1637) and OASTL (Gh_A13G0863)] during synthesis of GSH from SO4 2−. Furthermore, we also observed a crosstalk of the hormones and TFs (transcription factors) enriched in hormone signal transduction pathway. Genes related to IAA exceeds the rest of hormones followed by ubiquitin related genes which are greater than TFs. The analysis of the expression profiles of diverse tissues under Na2SO4 stress and identification of relevant key hub genes in a network crosstalk will provide a strong foundation and valuable clues for genetic improvements of cotton in response to various salt stresses.
format	article
author	Qinqin Wang Xuke Lu Xiugui Chen Waqar Afzal Malik Delong Wang Lanjie Zhao Junjuan Wang Shuai Wang Lixue Guo Ruifeng Cui Mingge Han Cun Rui Yuexin Zhang Yapeng Fan Chao Chen Wuwei Ye
author_facet	Qinqin Wang Xuke Lu Xiugui Chen Waqar Afzal Malik Delong Wang Lanjie Zhao Junjuan Wang Shuai Wang Lixue Guo Ruifeng Cui Mingge Han Cun Rui Yuexin Zhang Yapeng Fan Chao Chen Wuwei Ye
author_sort	Qinqin Wang
title	Transcriptome analysis of upland cotton revealed novel pathways to scavenge reactive oxygen species (ROS) responding to Na2SO4 tolerance
title_short	Transcriptome analysis of upland cotton revealed novel pathways to scavenge reactive oxygen species (ROS) responding to Na2SO4 tolerance
title_full	Transcriptome analysis of upland cotton revealed novel pathways to scavenge reactive oxygen species (ROS) responding to Na2SO4 tolerance
title_fullStr	Transcriptome analysis of upland cotton revealed novel pathways to scavenge reactive oxygen species (ROS) responding to Na2SO4 tolerance
title_full_unstemmed	Transcriptome analysis of upland cotton revealed novel pathways to scavenge reactive oxygen species (ROS) responding to Na2SO4 tolerance
title_sort	transcriptome analysis of upland cotton revealed novel pathways to scavenge reactive oxygen species (ros) responding to na2so4 tolerance
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/67453cc4284841bbb9df332d0750a02f
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Transcriptome analysis of upland cotton revealed novel pathways to scavenge reactive oxygen species (ROS) responding to Na2SO4 tolerance

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