Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm

Abstract Basal stem rot (BSR) of oil palm is a disastrous disease caused by a white-rot fungus Ganoderma boninense Pat. Non-ribosomal peptides (NRPs) synthesized by non-ribosomal peptide synthetases (NRPSs) are a group of secondary metabolites that act as fungal virulent factors during pathogenesis...

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Autores principales:	Neda Shokrollahi, Chai-Ling Ho, Nur Ain Izzati Mohd Zainudin, Mohd As’wad Bin Abul Wahab, Mui-Yun Wong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6749b555e6a84ffda590e7092c8f3120
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spelling	oai:doaj.org-article:6749b555e6a84ffda590e7092c8f31202021-12-02T16:27:50ZIdentification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm10.1038/s41598-021-95549-82045-2322https://doaj.org/article/6749b555e6a84ffda590e7092c8f31202021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95549-8https://doaj.org/toc/2045-2322Abstract Basal stem rot (BSR) of oil palm is a disastrous disease caused by a white-rot fungus Ganoderma boninense Pat. Non-ribosomal peptides (NRPs) synthesized by non-ribosomal peptide synthetases (NRPSs) are a group of secondary metabolites that act as fungal virulent factors during pathogenesis in the host. In this study, we aimed to isolate NRPS gene of G. boninense strain UPMGB001 and investigate the role of this gene during G. boninense-oil palm interaction. The isolated NRPS DNA fragment of 8322 bp was used to predict the putative peptide sequence of different domains and showed similarity with G. sinense (85%) at conserved motifs of three main NRPS domains. Phylogenetic analysis of NRPS peptide sequences demonstrated that NRPS of G. boninense belongs to the type VI siderophore family. The roots of 6-month-old oil palm seedlings were artificially inoculated for studying NRPS gene expression and disease severity in the greenhouse. The correlation between high disease severity (50%) and high expression (67-fold) of G. boninense NRPS gene at 4 months after inoculation and above indicated that this gene played a significant role in the advancement of BSR disease. Overall, these findings increase our knowledge on the gene structure of NRPS in G. boninense and its involvement in BSR pathogenesis as an effector gene.Neda ShokrollahiChai-Ling HoNur Ain Izzati Mohd ZainudinMohd As’wad Bin Abul WahabMui-Yun WongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Neda Shokrollahi Chai-Ling Ho Nur Ain Izzati Mohd Zainudin Mohd As’wad Bin Abul Wahab Mui-Yun Wong Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm
description	Abstract Basal stem rot (BSR) of oil palm is a disastrous disease caused by a white-rot fungus Ganoderma boninense Pat. Non-ribosomal peptides (NRPs) synthesized by non-ribosomal peptide synthetases (NRPSs) are a group of secondary metabolites that act as fungal virulent factors during pathogenesis in the host. In this study, we aimed to isolate NRPS gene of G. boninense strain UPMGB001 and investigate the role of this gene during G. boninense-oil palm interaction. The isolated NRPS DNA fragment of 8322 bp was used to predict the putative peptide sequence of different domains and showed similarity with G. sinense (85%) at conserved motifs of three main NRPS domains. Phylogenetic analysis of NRPS peptide sequences demonstrated that NRPS of G. boninense belongs to the type VI siderophore family. The roots of 6-month-old oil palm seedlings were artificially inoculated for studying NRPS gene expression and disease severity in the greenhouse. The correlation between high disease severity (50%) and high expression (67-fold) of G. boninense NRPS gene at 4 months after inoculation and above indicated that this gene played a significant role in the advancement of BSR disease. Overall, these findings increase our knowledge on the gene structure of NRPS in G. boninense and its involvement in BSR pathogenesis as an effector gene.
format	article
author	Neda Shokrollahi Chai-Ling Ho Nur Ain Izzati Mohd Zainudin Mohd As’wad Bin Abul Wahab Mui-Yun Wong
author_facet	Neda Shokrollahi Chai-Ling Ho Nur Ain Izzati Mohd Zainudin Mohd As’wad Bin Abul Wahab Mui-Yun Wong
author_sort	Neda Shokrollahi
title	Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm
title_short	Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm
title_full	Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm
title_fullStr	Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm
title_full_unstemmed	Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm
title_sort	identification of non-ribosomal peptide synthetase in ganoderma boninense pat. that was expressed during the interaction with oil palm
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6749b555e6a84ffda590e7092c8f3120
work_keys_str_mv	AT nedashokrollahi identificationofnonribosomalpeptidesynthetaseinganodermaboninensepatthatwasexpressedduringtheinteractionwithoilpalm AT chailingho identificationofnonribosomalpeptidesynthetaseinganodermaboninensepatthatwasexpressedduringtheinteractionwithoilpalm AT nurainizzatimohdzainudin identificationofnonribosomalpeptidesynthetaseinganodermaboninensepatthatwasexpressedduringtheinteractionwithoilpalm AT mohdaswadbinabulwahab identificationofnonribosomalpeptidesynthetaseinganodermaboninensepatthatwasexpressedduringtheinteractionwithoilpalm AT muiyunwong identificationofnonribosomalpeptidesynthetaseinganodermaboninensepatthatwasexpressedduringtheinteractionwithoilpalm
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Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm

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