SOX2 is required for inner ear neurogenesis

Abstract Neurons of the cochleovestibular ganglion (CVG) transmit hearing and balance information to the brain. During development, a select population of early otic progenitors express NEUROG1, delaminate from the otocyst, and coalesce to form the neurons that innervate all inner ear sensory region...

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Autores principales:	Aleta R. Steevens, Danielle L. Sookiasian, Jenna C. Glatzer, Amy E. Kiernan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6749d50a70094a27bf463aab0b4541e3
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spelling	oai:doaj.org-article:6749d50a70094a27bf463aab0b4541e32021-12-02T15:05:38ZSOX2 is required for inner ear neurogenesis10.1038/s41598-017-04315-22045-2322https://doaj.org/article/6749d50a70094a27bf463aab0b4541e32017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04315-2https://doaj.org/toc/2045-2322Abstract Neurons of the cochleovestibular ganglion (CVG) transmit hearing and balance information to the brain. During development, a select population of early otic progenitors express NEUROG1, delaminate from the otocyst, and coalesce to form the neurons that innervate all inner ear sensory regions. At present, the selection process that determines which otic progenitors activate NEUROG1 and adopt a neuroblast fate is incompletely understood. The transcription factor SOX2 has been implicated in otic neurogenesis, but its requirement in the specification of the CVG neurons has not been established. Here we tested SOX2’s requirement during inner ear neuronal specification using a conditional deletion paradigm in the mouse. SOX2 deficiency at otocyst stages caused a near-absence of NEUROG1-expressing neuroblasts, increased cell death in the neurosensory epithelium, and significantly reduced the CVG volume. Interestingly, a milder decrease in neurogenesis was observed in heterozygotes, indicating SOX2 levels are important. Moreover, fate-mapping experiments revealed that the timing of SOX2 expression did not parallel the established vestibular-then-auditory sequence. These results demonstrate that SOX2 is required for the initial events in otic neuronal specification including expression of NEUROG1, although fate-mapping results suggest SOX2 may be required as a competence factor rather than a direct initiator of the neural fate.Aleta R. SteevensDanielle L. SookiasianJenna C. GlatzerAmy E. KiernanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aleta R. Steevens Danielle L. Sookiasian Jenna C. Glatzer Amy E. Kiernan SOX2 is required for inner ear neurogenesis
description	Abstract Neurons of the cochleovestibular ganglion (CVG) transmit hearing and balance information to the brain. During development, a select population of early otic progenitors express NEUROG1, delaminate from the otocyst, and coalesce to form the neurons that innervate all inner ear sensory regions. At present, the selection process that determines which otic progenitors activate NEUROG1 and adopt a neuroblast fate is incompletely understood. The transcription factor SOX2 has been implicated in otic neurogenesis, but its requirement in the specification of the CVG neurons has not been established. Here we tested SOX2’s requirement during inner ear neuronal specification using a conditional deletion paradigm in the mouse. SOX2 deficiency at otocyst stages caused a near-absence of NEUROG1-expressing neuroblasts, increased cell death in the neurosensory epithelium, and significantly reduced the CVG volume. Interestingly, a milder decrease in neurogenesis was observed in heterozygotes, indicating SOX2 levels are important. Moreover, fate-mapping experiments revealed that the timing of SOX2 expression did not parallel the established vestibular-then-auditory sequence. These results demonstrate that SOX2 is required for the initial events in otic neuronal specification including expression of NEUROG1, although fate-mapping results suggest SOX2 may be required as a competence factor rather than a direct initiator of the neural fate.
format	article
author	Aleta R. Steevens Danielle L. Sookiasian Jenna C. Glatzer Amy E. Kiernan
author_facet	Aleta R. Steevens Danielle L. Sookiasian Jenna C. Glatzer Amy E. Kiernan
author_sort	Aleta R. Steevens
title	SOX2 is required for inner ear neurogenesis
title_short	SOX2 is required for inner ear neurogenesis
title_full	SOX2 is required for inner ear neurogenesis
title_fullStr	SOX2 is required for inner ear neurogenesis
title_full_unstemmed	SOX2 is required for inner ear neurogenesis
title_sort	sox2 is required for inner ear neurogenesis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6749d50a70094a27bf463aab0b4541e3
work_keys_str_mv	AT aletarsteevens sox2isrequiredforinnerearneurogenesis AT daniellelsookiasian sox2isrequiredforinnerearneurogenesis AT jennacglatzer sox2isrequiredforinnerearneurogenesis AT amyekiernan sox2isrequiredforinnerearneurogenesis
_version_	1718388768870760448

SOX2 is required for inner ear neurogenesis

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