Multi-Enzyme Supplementation Modifies the Gut Microbiome and Metabolome in Breeding Hens

Laying and reproductive performance, egg quality, and disease resistance of hens decrease during the late laying period. Exogenous enzymes promote nutrient digestibility and utilization and improve the intestinal environment. However, the specific regulation of the gut microbiome and metabolome by e...

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Autores principales:	Yuchen Liu, Dan Zeng, Lujiang Qu, Zhong Wang, Zhonghua Ning
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	multi-enzyme aged layers immunity reproduction performance microbiome metabolome Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/67509a5b3f6843319b1e12d7381320a1
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spelling	oai:doaj.org-article:67509a5b3f6843319b1e12d7381320a12021-12-03T07:17:16ZMulti-Enzyme Supplementation Modifies the Gut Microbiome and Metabolome in Breeding Hens1664-302X10.3389/fmicb.2021.711905https://doaj.org/article/67509a5b3f6843319b1e12d7381320a12021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.711905/fullhttps://doaj.org/toc/1664-302XLaying and reproductive performance, egg quality, and disease resistance of hens decrease during the late laying period. Exogenous enzymes promote nutrient digestibility and utilization and improve the intestinal environment. However, the specific regulation of the gut microbiome and metabolome by exogenous enzymes remains unelucidated. This study was conducted to evaluate effects of dietary multi-enzyme supplementation on egg and reproductive performance, egg quality, ileum microbiome, and metabolome of breeders. Here, 224 Hy-Line Brown breeding hens (55 weeks old) were randomly allocated to two groups: dietary controls fed basal diet (DC), and test hens fed 0.2 g/kg corn enzyme diet (CE). Serum levels of total protein, globulin, immunoglobulin Y, and antibodies against the Newcastle disease virus and avian influenza H9 strain were significantly increased (p < 0.05). Egg albumen height, Haugh unit, and fertilization and hatching rates were also significantly increased (p < 0.05) in the CE-fed group. 16S rRNA sequence analysis showed that CE strongly affected both α- and β-diversity of the ileal microbiota. LEfSe analysis revealed that the potentially beneficial genera Lactobacillus, Enterococcus, Faecalicoccus, and Streptococcus were enriched as biomarkers in the CE-fed group. Microbial functional analysis revealed that the functional genes associated with harmful-substance biodegradation was significantly increased in the CE-fed group. Additionally, Spearman correlation analysis indicated that changes in microbial genera were correlated with differential metabolites. In summary, dietary multi-enzyme addition can improve egg quality, humoral immunity, and reproductive performance and regulate the intestinal microbiome and metabolome in breeders. Therefore, multi-enzymes could be used as feed additive to extend breeder service life.Yuchen LiuDan ZengLujiang QuZhong WangZhonghua NingFrontiers Media S.A.articlemulti-enzymeaged layersimmunityreproduction performancemicrobiomemetabolomeMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	multi-enzyme aged layers immunity reproduction performance microbiome metabolome Microbiology QR1-502
spellingShingle	multi-enzyme aged layers immunity reproduction performance microbiome metabolome Microbiology QR1-502 Yuchen Liu Dan Zeng Lujiang Qu Zhong Wang Zhonghua Ning Multi-Enzyme Supplementation Modifies the Gut Microbiome and Metabolome in Breeding Hens
description	Laying and reproductive performance, egg quality, and disease resistance of hens decrease during the late laying period. Exogenous enzymes promote nutrient digestibility and utilization and improve the intestinal environment. However, the specific regulation of the gut microbiome and metabolome by exogenous enzymes remains unelucidated. This study was conducted to evaluate effects of dietary multi-enzyme supplementation on egg and reproductive performance, egg quality, ileum microbiome, and metabolome of breeders. Here, 224 Hy-Line Brown breeding hens (55 weeks old) were randomly allocated to two groups: dietary controls fed basal diet (DC), and test hens fed 0.2 g/kg corn enzyme diet (CE). Serum levels of total protein, globulin, immunoglobulin Y, and antibodies against the Newcastle disease virus and avian influenza H9 strain were significantly increased (p < 0.05). Egg albumen height, Haugh unit, and fertilization and hatching rates were also significantly increased (p < 0.05) in the CE-fed group. 16S rRNA sequence analysis showed that CE strongly affected both α- and β-diversity of the ileal microbiota. LEfSe analysis revealed that the potentially beneficial genera Lactobacillus, Enterococcus, Faecalicoccus, and Streptococcus were enriched as biomarkers in the CE-fed group. Microbial functional analysis revealed that the functional genes associated with harmful-substance biodegradation was significantly increased in the CE-fed group. Additionally, Spearman correlation analysis indicated that changes in microbial genera were correlated with differential metabolites. In summary, dietary multi-enzyme addition can improve egg quality, humoral immunity, and reproductive performance and regulate the intestinal microbiome and metabolome in breeders. Therefore, multi-enzymes could be used as feed additive to extend breeder service life.
format	article
author	Yuchen Liu Dan Zeng Lujiang Qu Zhong Wang Zhonghua Ning
author_facet	Yuchen Liu Dan Zeng Lujiang Qu Zhong Wang Zhonghua Ning
author_sort	Yuchen Liu
title	Multi-Enzyme Supplementation Modifies the Gut Microbiome and Metabolome in Breeding Hens
title_short	Multi-Enzyme Supplementation Modifies the Gut Microbiome and Metabolome in Breeding Hens
title_full	Multi-Enzyme Supplementation Modifies the Gut Microbiome and Metabolome in Breeding Hens
title_fullStr	Multi-Enzyme Supplementation Modifies the Gut Microbiome and Metabolome in Breeding Hens
title_full_unstemmed	Multi-Enzyme Supplementation Modifies the Gut Microbiome and Metabolome in Breeding Hens
title_sort	multi-enzyme supplementation modifies the gut microbiome and metabolome in breeding hens
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/67509a5b3f6843319b1e12d7381320a1
work_keys_str_mv	AT yuchenliu multienzymesupplementationmodifiesthegutmicrobiomeandmetabolomeinbreedinghens AT danzeng multienzymesupplementationmodifiesthegutmicrobiomeandmetabolomeinbreedinghens AT lujiangqu multienzymesupplementationmodifiesthegutmicrobiomeandmetabolomeinbreedinghens AT zhongwang multienzymesupplementationmodifiesthegutmicrobiomeandmetabolomeinbreedinghens AT zhonghuaning multienzymesupplementationmodifiesthegutmicrobiomeandmetabolomeinbreedinghens
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Multi-Enzyme Supplementation Modifies the Gut Microbiome and Metabolome in Breeding Hens

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