Serum albumin and osmolality inhibit Bdellovibrio bacteriovorus predation in human serum

Abstract We evaluated the bactericidal activity of Bdellovibrio bacteriovorus, strain HD100, within blood sera against bacterial strains commonly associated with bacteremic infections, including E. coli, Klebsiella pneumoniae and Salmonella enterica. Tests show that B. bacteriovorus HD100 is not sus...

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Autores principales:	Hansol Im, Sangmo Son, Robert J. Mitchell, Cheol-Min Ghim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/67510b910c4f42bfb98bfbd63a428ee4
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spelling	oai:doaj.org-article:67510b910c4f42bfb98bfbd63a428ee42021-12-02T11:53:05ZSerum albumin and osmolality inhibit Bdellovibrio bacteriovorus predation in human serum10.1038/s41598-017-06272-22045-2322https://doaj.org/article/67510b910c4f42bfb98bfbd63a428ee42017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06272-2https://doaj.org/toc/2045-2322Abstract We evaluated the bactericidal activity of Bdellovibrio bacteriovorus, strain HD100, within blood sera against bacterial strains commonly associated with bacteremic infections, including E. coli, Klebsiella pneumoniae and Salmonella enterica. Tests show that B. bacteriovorus HD100 is not susceptible to serum complement or its bactericidal activity. After a two hour exposure to human sera, the prey populations decreased 15- to 7,300-fold due to the serum complement activity while, in contrast, the B. bacteriovorus HD100 population showed a loss of only 33%. Dot blot analyses showed that this is not due to the absence of antibodies against this predator. Predation in human serum was inhibited, though, by both the osmolality and serum albumin. The activity of B. bacteriovorus HD100 showed a sharp transition between 200 and 250 mOsm/kg, and was progressively reduced as the osmolality increased. Serum albumin also acted to inhibit predation by binding to and coating the predatory cells. This was confirmed via dot blot analyses and confocal microscopy. The results from both the osmolality and serum albumin tests were incorporated into a numerical model describing bacterial predation of pathogens. In conclusion, both of these factors inhibit predation and, as such, they limit its effectiveness against pathogenic prey located within sera.Hansol ImSangmo SonRobert J. MitchellCheol-Min GhimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hansol Im Sangmo Son Robert J. Mitchell Cheol-Min Ghim Serum albumin and osmolality inhibit Bdellovibrio bacteriovorus predation in human serum
description	Abstract We evaluated the bactericidal activity of Bdellovibrio bacteriovorus, strain HD100, within blood sera against bacterial strains commonly associated with bacteremic infections, including E. coli, Klebsiella pneumoniae and Salmonella enterica. Tests show that B. bacteriovorus HD100 is not susceptible to serum complement or its bactericidal activity. After a two hour exposure to human sera, the prey populations decreased 15- to 7,300-fold due to the serum complement activity while, in contrast, the B. bacteriovorus HD100 population showed a loss of only 33%. Dot blot analyses showed that this is not due to the absence of antibodies against this predator. Predation in human serum was inhibited, though, by both the osmolality and serum albumin. The activity of B. bacteriovorus HD100 showed a sharp transition between 200 and 250 mOsm/kg, and was progressively reduced as the osmolality increased. Serum albumin also acted to inhibit predation by binding to and coating the predatory cells. This was confirmed via dot blot analyses and confocal microscopy. The results from both the osmolality and serum albumin tests were incorporated into a numerical model describing bacterial predation of pathogens. In conclusion, both of these factors inhibit predation and, as such, they limit its effectiveness against pathogenic prey located within sera.
format	article
author	Hansol Im Sangmo Son Robert J. Mitchell Cheol-Min Ghim
author_facet	Hansol Im Sangmo Son Robert J. Mitchell Cheol-Min Ghim
author_sort	Hansol Im
title	Serum albumin and osmolality inhibit Bdellovibrio bacteriovorus predation in human serum
title_short	Serum albumin and osmolality inhibit Bdellovibrio bacteriovorus predation in human serum
title_full	Serum albumin and osmolality inhibit Bdellovibrio bacteriovorus predation in human serum
title_fullStr	Serum albumin and osmolality inhibit Bdellovibrio bacteriovorus predation in human serum
title_full_unstemmed	Serum albumin and osmolality inhibit Bdellovibrio bacteriovorus predation in human serum
title_sort	serum albumin and osmolality inhibit bdellovibrio bacteriovorus predation in human serum
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/67510b910c4f42bfb98bfbd63a428ee4
work_keys_str_mv	AT hansolim serumalbuminandosmolalityinhibitbdellovibriobacteriovoruspredationinhumanserum AT sangmoson serumalbuminandosmolalityinhibitbdellovibriobacteriovoruspredationinhumanserum AT robertjmitchell serumalbuminandosmolalityinhibitbdellovibriobacteriovoruspredationinhumanserum AT cheolminghim serumalbuminandosmolalityinhibitbdellovibriobacteriovoruspredationinhumanserum
_version_	1718394870387703808

Serum albumin and osmolality inhibit Bdellovibrio bacteriovorus predation in human serum

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