A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells

Determining the trafficking of intracellular material is commonly done by colocalisation analysis using microscopy. Here the authors monitor trafficking of select cargo by measuring the conversion of quenched SNAP-tag substrates by subcellularly-localised SNAP-tag and detection by flow cytometry.

Guardado en:

Detalles Bibliográficos
Autores principales:	Laura I. FitzGerald, Luigi Aurelio, Moore Chen, Daniel Yuen, Joshua J. Rennick, Bim Graham, Angus P. R. Johnston
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/67585245818b4c449e5738f29dc6b93c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:67585245818b4c449e5738f29dc6b93c
record_format	dspace
spelling	oai:doaj.org-article:67585245818b4c449e5738f29dc6b93c2021-12-02T17:19:42ZA molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells10.1038/s41467-020-18082-82041-1723https://doaj.org/article/67585245818b4c449e5738f29dc6b93c2020-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18082-8https://doaj.org/toc/2041-1723Determining the trafficking of intracellular material is commonly done by colocalisation analysis using microscopy. Here the authors monitor trafficking of select cargo by measuring the conversion of quenched SNAP-tag substrates by subcellularly-localised SNAP-tag and detection by flow cytometry.Laura I. FitzGeraldLuigi AurelioMoore ChenDaniel YuenJoshua J. RennickBim GrahamAngus P. R. JohnstonNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Laura I. FitzGerald Luigi Aurelio Moore Chen Daniel Yuen Joshua J. Rennick Bim Graham Angus P. R. Johnston A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells
description	Determining the trafficking of intracellular material is commonly done by colocalisation analysis using microscopy. Here the authors monitor trafficking of select cargo by measuring the conversion of quenched SNAP-tag substrates by subcellularly-localised SNAP-tag and detection by flow cytometry.
format	article
author	Laura I. FitzGerald Luigi Aurelio Moore Chen Daniel Yuen Joshua J. Rennick Bim Graham Angus P. R. Johnston
author_facet	Laura I. FitzGerald Luigi Aurelio Moore Chen Daniel Yuen Joshua J. Rennick Bim Graham Angus P. R. Johnston
author_sort	Laura I. FitzGerald
title	A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells
title_short	A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells
title_full	A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells
title_fullStr	A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells
title_full_unstemmed	A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells
title_sort	molecular sensor to quantify the localization of proteins, dna and nanoparticles in cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/67585245818b4c449e5738f29dc6b93c
work_keys_str_mv	AT lauraifitzgerald amolecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells AT luigiaurelio amolecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells AT moorechen amolecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells AT danielyuen amolecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells AT joshuajrennick amolecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells AT bimgraham amolecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells AT angusprjohnston amolecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells AT lauraifitzgerald molecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells AT luigiaurelio molecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells AT moorechen molecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells AT danielyuen molecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells AT joshuajrennick molecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells AT bimgraham molecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells AT angusprjohnston molecularsensortoquantifythelocalizationofproteinsdnaandnanoparticlesincells
_version_	1718381030174359552

A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells

Ejemplares similares