A molecular sensor to quantify the localization of proteins, DNA and nanoparticles in cells
Determining the trafficking of intracellular material is commonly done by colocalisation analysis using microscopy. Here the authors monitor trafficking of select cargo by measuring the conversion of quenched SNAP-tag substrates by subcellularly-localised SNAP-tag and detection by flow cytometry.
Guardado en:
Autores principales: | Laura I. FitzGerald, Luigi Aurelio, Moore Chen, Daniel Yuen, Joshua J. Rennick, Bim Graham, Angus P. R. Johnston |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2020
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Materias: | |
Acceso en línea: | https://doaj.org/article/67585245818b4c449e5738f29dc6b93c |
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