A recombinant influenza A virus expressing domain III of West Nile virus induces protective immune responses against influenza and West Nile virus.

West Nile virus (WNV) continues to circulate in the USA and forms a threat to the rest of the Western hemisphere. Since methods for the treatment of WNV infections are not available, there is a need for the development of safe and effective vaccines. Here, we describe the construction of a recombina...

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Autores principales:	Byron E E Martina, Petra van den Doel, Penelope Koraka, Geert van Amerongen, Gunther Spohn, Bart L Haagmans, Lisette B V Provacia, Albert D M E Osterhaus, Guus F Rimmelzwaan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/676a1871368e4c7bad7fb9116614ffe0
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spelling	oai:doaj.org-article:676a1871368e4c7bad7fb9116614ffe02021-11-18T06:55:09ZA recombinant influenza A virus expressing domain III of West Nile virus induces protective immune responses against influenza and West Nile virus.1932-620310.1371/journal.pone.0018995https://doaj.org/article/676a1871368e4c7bad7fb9116614ffe02011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21541326/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203West Nile virus (WNV) continues to circulate in the USA and forms a threat to the rest of the Western hemisphere. Since methods for the treatment of WNV infections are not available, there is a need for the development of safe and effective vaccines. Here, we describe the construction of a recombinant influenza virus expressing domain III of the WNV glycoprotein E (Flu-NA-DIII) and its evaluation as a WNV vaccine candidate in a mouse model. FLU-NA-DIII-vaccinated mice were protected from severe body weight loss and mortality caused by WNV infection, whereas control mice succumbed to the infection. In addition, it was shown that one subcutaneous immunization with 10(5) TCID(50) Flu-NA-DIII provided 100% protection against challenge. Adoptive transfer experiments demonstrated that protection was mediated by antibodies and CD4+T cells. Furthermore, mice vaccinated with FLU-NA-DIII developed protective influenza virus-specific antibody titers. It was concluded that this vector system might be an attractive platform for the development of bivalent WNV-influenza vaccines.Byron E E MartinaPetra van den DoelPenelope KorakaGeert van AmerongenGunther SpohnBart L HaagmansLisette B V ProvaciaAlbert D M E OsterhausGuus F RimmelzwaanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18995 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Byron E E Martina Petra van den Doel Penelope Koraka Geert van Amerongen Gunther Spohn Bart L Haagmans Lisette B V Provacia Albert D M E Osterhaus Guus F Rimmelzwaan A recombinant influenza A virus expressing domain III of West Nile virus induces protective immune responses against influenza and West Nile virus.
description	West Nile virus (WNV) continues to circulate in the USA and forms a threat to the rest of the Western hemisphere. Since methods for the treatment of WNV infections are not available, there is a need for the development of safe and effective vaccines. Here, we describe the construction of a recombinant influenza virus expressing domain III of the WNV glycoprotein E (Flu-NA-DIII) and its evaluation as a WNV vaccine candidate in a mouse model. FLU-NA-DIII-vaccinated mice were protected from severe body weight loss and mortality caused by WNV infection, whereas control mice succumbed to the infection. In addition, it was shown that one subcutaneous immunization with 10(5) TCID(50) Flu-NA-DIII provided 100% protection against challenge. Adoptive transfer experiments demonstrated that protection was mediated by antibodies and CD4+T cells. Furthermore, mice vaccinated with FLU-NA-DIII developed protective influenza virus-specific antibody titers. It was concluded that this vector system might be an attractive platform for the development of bivalent WNV-influenza vaccines.
format	article
author	Byron E E Martina Petra van den Doel Penelope Koraka Geert van Amerongen Gunther Spohn Bart L Haagmans Lisette B V Provacia Albert D M E Osterhaus Guus F Rimmelzwaan
author_facet	Byron E E Martina Petra van den Doel Penelope Koraka Geert van Amerongen Gunther Spohn Bart L Haagmans Lisette B V Provacia Albert D M E Osterhaus Guus F Rimmelzwaan
author_sort	Byron E E Martina
title	A recombinant influenza A virus expressing domain III of West Nile virus induces protective immune responses against influenza and West Nile virus.
title_short	A recombinant influenza A virus expressing domain III of West Nile virus induces protective immune responses against influenza and West Nile virus.
title_full	A recombinant influenza A virus expressing domain III of West Nile virus induces protective immune responses against influenza and West Nile virus.
title_fullStr	A recombinant influenza A virus expressing domain III of West Nile virus induces protective immune responses against influenza and West Nile virus.
title_full_unstemmed	A recombinant influenza A virus expressing domain III of West Nile virus induces protective immune responses against influenza and West Nile virus.
title_sort	recombinant influenza a virus expressing domain iii of west nile virus induces protective immune responses against influenza and west nile virus.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/676a1871368e4c7bad7fb9116614ffe0
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A recombinant influenza A virus expressing domain III of West Nile virus induces protective immune responses against influenza and West Nile virus.

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