Enhancer analysis of the Drosophila zinc finger transcription factor Earmuff by gene targeting

Abstract Background Many transcription factors are involved in the formation of the brain during the development of Drosophila melanogaster. The transcription factor Earmuff (Erm), a member of the forebrain embryonic zinc finger family (Fezf), is one of these important factors for brain development....

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Autores principales:	Kirsten Hildebrandt, Sabrina Kübel, Marie Minet, Nora Fürst, Christine Klöppel, Eva Steinmetz, Uwe Walldorf
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Earmuff (Erm) Transcription factor Enhancer Gene targeting Genetics QH426-470
Acceso en línea:	https://doaj.org/article/676c2f54a7c9419c817bc9545dea54d6
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spelling	oai:doaj.org-article:676c2f54a7c9419c817bc9545dea54d62021-11-07T12:15:29ZEnhancer analysis of the Drosophila zinc finger transcription factor Earmuff by gene targeting10.1186/s41065-021-00209-61601-5223https://doaj.org/article/676c2f54a7c9419c817bc9545dea54d62021-11-01T00:00:00Zhttps://doi.org/10.1186/s41065-021-00209-6https://doaj.org/toc/1601-5223Abstract Background Many transcription factors are involved in the formation of the brain during the development of Drosophila melanogaster. The transcription factor Earmuff (Erm), a member of the forebrain embryonic zinc finger family (Fezf), is one of these important factors for brain development. One major function of Earmuff is the regulation of proliferation within type II neuroblast lineages in the brain; here, Earmuff is expressed in intermediate neural progenitor cells (INPs) and balances neuronal differentiation versus stem cell maintenance. Erm expression during development is regulated by several enhancers. Results In this work we show a functional analysis of erm and some of its enhancers. We generated a new erm mutant allele by gene targeting and reintegrated Gal4 to make an erm enhancer trap strain that could also be used on an erm mutant background. The deletion of three of the previously analysed enhancers showing the most prominent expression patterns of erm by gene targeting resulted in specific temporal and spatial defects in defined brain structures. These defects were already known but here could be assigned to specific enhancer regions. Conclusion This analysis is to our knowledge the first systematic analysis of several large enhancer deletions of a Drosophila gene by gene targeting and will enable deeper analysis of erm enhancer functions in the future.Kirsten HildebrandtSabrina KübelMarie MinetNora FürstChristine KlöppelEva SteinmetzUwe WalldorfBMCarticleEarmuff (Erm)Transcription factorEnhancerGene targetingGeneticsQH426-470ENHereditas, Vol 158, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Earmuff (Erm) Transcription factor Enhancer Gene targeting Genetics QH426-470
spellingShingle	Earmuff (Erm) Transcription factor Enhancer Gene targeting Genetics QH426-470 Kirsten Hildebrandt Sabrina Kübel Marie Minet Nora Fürst Christine Klöppel Eva Steinmetz Uwe Walldorf Enhancer analysis of the Drosophila zinc finger transcription factor Earmuff by gene targeting
description	Abstract Background Many transcription factors are involved in the formation of the brain during the development of Drosophila melanogaster. The transcription factor Earmuff (Erm), a member of the forebrain embryonic zinc finger family (Fezf), is one of these important factors for brain development. One major function of Earmuff is the regulation of proliferation within type II neuroblast lineages in the brain; here, Earmuff is expressed in intermediate neural progenitor cells (INPs) and balances neuronal differentiation versus stem cell maintenance. Erm expression during development is regulated by several enhancers. Results In this work we show a functional analysis of erm and some of its enhancers. We generated a new erm mutant allele by gene targeting and reintegrated Gal4 to make an erm enhancer trap strain that could also be used on an erm mutant background. The deletion of three of the previously analysed enhancers showing the most prominent expression patterns of erm by gene targeting resulted in specific temporal and spatial defects in defined brain structures. These defects were already known but here could be assigned to specific enhancer regions. Conclusion This analysis is to our knowledge the first systematic analysis of several large enhancer deletions of a Drosophila gene by gene targeting and will enable deeper analysis of erm enhancer functions in the future.
format	article
author	Kirsten Hildebrandt Sabrina Kübel Marie Minet Nora Fürst Christine Klöppel Eva Steinmetz Uwe Walldorf
author_facet	Kirsten Hildebrandt Sabrina Kübel Marie Minet Nora Fürst Christine Klöppel Eva Steinmetz Uwe Walldorf
author_sort	Kirsten Hildebrandt
title	Enhancer analysis of the Drosophila zinc finger transcription factor Earmuff by gene targeting
title_short	Enhancer analysis of the Drosophila zinc finger transcription factor Earmuff by gene targeting
title_full	Enhancer analysis of the Drosophila zinc finger transcription factor Earmuff by gene targeting
title_fullStr	Enhancer analysis of the Drosophila zinc finger transcription factor Earmuff by gene targeting
title_full_unstemmed	Enhancer analysis of the Drosophila zinc finger transcription factor Earmuff by gene targeting
title_sort	enhancer analysis of the drosophila zinc finger transcription factor earmuff by gene targeting
publisher	BMC
publishDate	2021
url	https://doaj.org/article/676c2f54a7c9419c817bc9545dea54d6
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Enhancer analysis of the Drosophila zinc finger transcription factor Earmuff by gene targeting

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