A flow platform for degradation-free CuAAC bioconjugation

Cu-catalyzed azide-alkyne cycloaddition (CuAAC) reaction is a common bioconjugation technique; however oxidative degradation and residual copper may limit its use. Here, the authors show that ppm levels of Cu, generated in situ by solvent-mediated pipe erosion in flow, catalyze the CuAAC reaction wi...

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Autores principales:	Marine Z. C. Hatit, Linus F. Reichenbach, John M. Tobin, Filipe Vilela, Glenn A. Burley, Allan J. B. Watson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/677a409525194539ad2bf5ef41b319c1
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id	oai:doaj.org-article:677a409525194539ad2bf5ef41b319c1
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spelling	oai:doaj.org-article:677a409525194539ad2bf5ef41b319c12021-12-02T17:31:12ZA flow platform for degradation-free CuAAC bioconjugation10.1038/s41467-018-06551-02041-1723https://doaj.org/article/677a409525194539ad2bf5ef41b319c12018-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06551-0https://doaj.org/toc/2041-1723Cu-catalyzed azide-alkyne cycloaddition (CuAAC) reaction is a common bioconjugation technique; however oxidative degradation and residual copper may limit its use. Here, the authors show that ppm levels of Cu, generated in situ by solvent-mediated pipe erosion in flow, catalyze the CuAAC reaction with no product degradation.Marine Z. C. HatitLinus F. ReichenbachJohn M. TobinFilipe VilelaGlenn A. BurleyAllan J. B. WatsonNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Marine Z. C. Hatit Linus F. Reichenbach John M. Tobin Filipe Vilela Glenn A. Burley Allan J. B. Watson A flow platform for degradation-free CuAAC bioconjugation
description	Cu-catalyzed azide-alkyne cycloaddition (CuAAC) reaction is a common bioconjugation technique; however oxidative degradation and residual copper may limit its use. Here, the authors show that ppm levels of Cu, generated in situ by solvent-mediated pipe erosion in flow, catalyze the CuAAC reaction with no product degradation.
format	article
author	Marine Z. C. Hatit Linus F. Reichenbach John M. Tobin Filipe Vilela Glenn A. Burley Allan J. B. Watson
author_facet	Marine Z. C. Hatit Linus F. Reichenbach John M. Tobin Filipe Vilela Glenn A. Burley Allan J. B. Watson
author_sort	Marine Z. C. Hatit
title	A flow platform for degradation-free CuAAC bioconjugation
title_short	A flow platform for degradation-free CuAAC bioconjugation
title_full	A flow platform for degradation-free CuAAC bioconjugation
title_fullStr	A flow platform for degradation-free CuAAC bioconjugation
title_full_unstemmed	A flow platform for degradation-free CuAAC bioconjugation
title_sort	flow platform for degradation-free cuaac bioconjugation
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/677a409525194539ad2bf5ef41b319c1
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A flow platform for degradation-free CuAAC bioconjugation

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