Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment

Currently tuberculosis is considered as a group of diseases united by one etiological factor. The pathogenesis of certain localizations of tuberculous inflammation, in particular peritoneum tuberculosis, hasn’t been sufficiently studied. The role of cytokine mechanisms in the development of the dise...

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Autores principales:	D. V. Plotkin, T. I. Vinogradova, M. N. Reshetnikov, Yu. R. Zyuzya, S. V. Okovityi, M. V. Sinitsyn, V. R. Gaitukaev, G. V. Rodoman, E. M. Bogorodskaya, P. K. Yablonskiy
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2021
Materias:	tuberculous peritonitis rabbit tumor necrosis factor model of tuberculous peritonitis infliximab Science Q
Acceso en línea:	https://doaj.org/article/677aa0645f874708a609b79c51904b5b
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spelling	oai:doaj.org-article:677aa0645f874708a609b79c51904b5b2021-11-23T06:14:47ZPathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment2541-94202587-959610.29413/ABS.2021-6.5.18https://doaj.org/article/677aa0645f874708a609b79c51904b5b2021-11-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/3049https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Currently tuberculosis is considered as a group of diseases united by one etiological factor. The pathogenesis of certain localizations of tuberculous inflammation, in particular peritoneum tuberculosis, hasn’t been sufficiently studied. The role of cytokine mechanisms in the development of the disease and the elaboration of non-sterile immunity requires further experimental studies, in particular the creation of a reproducible model on laboratory animals.The aim: to study the effect of TNF-α on the development of tuberculosis of the serous coat of the abdominal cavity, as well as to evaluate the possibility of modeling tuberculous peritonitis in laboratory animals using infliximab.Materials and methods. The studies were conducted on 18 male rabbits, which were simulated peritoneal tuberculosis by intra-abdominal administration of a suspension of Mycobacterium tuberculosis. 10 rabbits of the experimental group were intravenously injected with an infliximab solution and an iron (III) hydroxide sucrose complex intraperitoneally a day before infection.Results. In the control group of animals, tuberculosis either didn’t develop, or in a third of cases it affected only the pulmonary parenchyma, while proliferative processes prevailed. On the contrary, in animals with inactivated TNF-α, in 100 % of observations, tuberculous peritonitis was detected with associated lung damage and the predominance of alterative caseous processes.Conclusion. The created model of tuberculous peritonitis shows the leading role of TNF-α in the activation of macrophages, as well as in attracting cells to the site of infection. This is the primary signal necessary for the formation and stability of granulomas since the neutralization of this cytokine leads to a loss of control over the infection and the destruction of the granuloma with the development of destructive tuberculosis in the serous coat of the abdominal cavity.D. V. PlotkinT. I. VinogradovaM. N. ReshetnikovYu. R. ZyuzyaS. V. OkovityiM. V. SinitsynV. R. GaitukaevG. V. RodomanE. M. BogorodskayaP. K. YablonskiyScientific Сentre for Family Health and Human Reproduction Problemsarticletuberculous peritonitisrabbittumor necrosis factormodel of tuberculous peritonitisinfliximabScienceQRUActa Biomedica Scientifica, Vol 6, Iss 5, Pp 184-195 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	tuberculous peritonitis rabbit tumor necrosis factor model of tuberculous peritonitis infliximab Science Q
spellingShingle	tuberculous peritonitis rabbit tumor necrosis factor model of tuberculous peritonitis infliximab Science Q D. V. Plotkin T. I. Vinogradova M. N. Reshetnikov Yu. R. Zyuzya S. V. Okovityi M. V. Sinitsyn V. R. Gaitukaev G. V. Rodoman E. M. Bogorodskaya P. K. Yablonskiy Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment
description	Currently tuberculosis is considered as a group of diseases united by one etiological factor. The pathogenesis of certain localizations of tuberculous inflammation, in particular peritoneum tuberculosis, hasn’t been sufficiently studied. The role of cytokine mechanisms in the development of the disease and the elaboration of non-sterile immunity requires further experimental studies, in particular the creation of a reproducible model on laboratory animals.The aim: to study the effect of TNF-α on the development of tuberculosis of the serous coat of the abdominal cavity, as well as to evaluate the possibility of modeling tuberculous peritonitis in laboratory animals using infliximab.Materials and methods. The studies were conducted on 18 male rabbits, which were simulated peritoneal tuberculosis by intra-abdominal administration of a suspension of Mycobacterium tuberculosis. 10 rabbits of the experimental group were intravenously injected with an infliximab solution and an iron (III) hydroxide sucrose complex intraperitoneally a day before infection.Results. In the control group of animals, tuberculosis either didn’t develop, or in a third of cases it affected only the pulmonary parenchyma, while proliferative processes prevailed. On the contrary, in animals with inactivated TNF-α, in 100 % of observations, tuberculous peritonitis was detected with associated lung damage and the predominance of alterative caseous processes.Conclusion. The created model of tuberculous peritonitis shows the leading role of TNF-α in the activation of macrophages, as well as in attracting cells to the site of infection. This is the primary signal necessary for the formation and stability of granulomas since the neutralization of this cytokine leads to a loss of control over the infection and the destruction of the granuloma with the development of destructive tuberculosis in the serous coat of the abdominal cavity.
format	article
author	D. V. Plotkin T. I. Vinogradova M. N. Reshetnikov Yu. R. Zyuzya S. V. Okovityi M. V. Sinitsyn V. R. Gaitukaev G. V. Rodoman E. M. Bogorodskaya P. K. Yablonskiy
author_facet	D. V. Plotkin T. I. Vinogradova M. N. Reshetnikov Yu. R. Zyuzya S. V. Okovityi M. V. Sinitsyn V. R. Gaitukaev G. V. Rodoman E. M. Bogorodskaya P. K. Yablonskiy
author_sort	D. V. Plotkin
title	Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment
title_short	Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment
title_full	Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment
title_fullStr	Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment
title_full_unstemmed	Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment
title_sort	pathogenetic role of tumor necrosis factor (tnf-α) for the development of peritoneal tuberculosis in an experiment
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2021
url	https://doaj.org/article/677aa0645f874708a609b79c51904b5b
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Pathogenetic role of tumor necrosis factor (TNF-α) for the development of peritoneal tuberculosis in an experiment

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