Regional Differences in mRNA and lncRNA Expression Profiles in Non-Failing Human Atria and Ventricles

Abstract The four chambers of the human heart play distinct roles in the maintenance of normal cardiac function, and are differentially affected by inherited/acquired cardiovascular disease. To probe the molecular determinants of these functional differences, we examined mRNA and lncRNA expression p...

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Autores principales:	Eric K. Johnson, Scot J. Matkovich, Jeanne M. Nerbonne
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	lncRNA Expression Profiles Paired Tissue Samples Myocardial Infarction Associated Transcript (MIAT) Unsupervised Hierarchical Clustering Differential Expression Analysis Medicine R Science Q
Acceso en línea:	https://doaj.org/article/677aef6ddd89489292f6b166481d1072
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spelling	oai:doaj.org-article:677aef6ddd89489292f6b166481d10722021-12-02T15:08:32ZRegional Differences in mRNA and lncRNA Expression Profiles in Non-Failing Human Atria and Ventricles10.1038/s41598-018-32154-22045-2322https://doaj.org/article/677aef6ddd89489292f6b166481d10722018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32154-2https://doaj.org/toc/2045-2322Abstract The four chambers of the human heart play distinct roles in the maintenance of normal cardiac function, and are differentially affected by inherited/acquired cardiovascular disease. To probe the molecular determinants of these functional differences, we examined mRNA and lncRNA expression profiles in the left (LA) and right (RA) atria, the left (LV) and right (RV) ventricles, and the interventricular septum (IVS) of non-failing human hearts (N = 8). Analysis of paired atrial and ventricular samples (n = 40) identified 5,747 mRNAs and 2,794 lncRNAs that were differentially (>1.5 fold; FDR < 0.05) expressed. The largest differences were observed in comparisons between the atrial (RA/LA) and ventricular (RV/LV/IVS) samples. In every case (e.g., LA vs LV, LA vs RV, etc.), >2,300 mRNAs and >1,200 lncRNAs, corresponding to 17–28% of the total transcripts, were differentially expressed. Heterogeneities in mRNA/lncRNA expression profiles in the LA and RA, as well as in the LV, RV and IVS, were also revealed, although the numbers of differentially expressed transcripts were substantially smaller. Gender differences in mRNA and lncRNA expression profiles were also evident in non-failing human atria and ventricles. Gene ontology classification of differentially expressed gene sets revealed chamber-specific enrichment of numerous signaling pathways.Eric K. JohnsonScot J. MatkovichJeanne M. NerbonneNature PortfolioarticlelncRNA Expression ProfilesPaired Tissue SamplesMyocardial Infarction Associated Transcript (MIAT)Unsupervised Hierarchical ClusteringDifferential Expression AnalysisMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	lncRNA Expression Profiles Paired Tissue Samples Myocardial Infarction Associated Transcript (MIAT) Unsupervised Hierarchical Clustering Differential Expression Analysis Medicine R Science Q
spellingShingle	lncRNA Expression Profiles Paired Tissue Samples Myocardial Infarction Associated Transcript (MIAT) Unsupervised Hierarchical Clustering Differential Expression Analysis Medicine R Science Q Eric K. Johnson Scot J. Matkovich Jeanne M. Nerbonne Regional Differences in mRNA and lncRNA Expression Profiles in Non-Failing Human Atria and Ventricles
description	Abstract The four chambers of the human heart play distinct roles in the maintenance of normal cardiac function, and are differentially affected by inherited/acquired cardiovascular disease. To probe the molecular determinants of these functional differences, we examined mRNA and lncRNA expression profiles in the left (LA) and right (RA) atria, the left (LV) and right (RV) ventricles, and the interventricular septum (IVS) of non-failing human hearts (N = 8). Analysis of paired atrial and ventricular samples (n = 40) identified 5,747 mRNAs and 2,794 lncRNAs that were differentially (>1.5 fold; FDR < 0.05) expressed. The largest differences were observed in comparisons between the atrial (RA/LA) and ventricular (RV/LV/IVS) samples. In every case (e.g., LA vs LV, LA vs RV, etc.), >2,300 mRNAs and >1,200 lncRNAs, corresponding to 17–28% of the total transcripts, were differentially expressed. Heterogeneities in mRNA/lncRNA expression profiles in the LA and RA, as well as in the LV, RV and IVS, were also revealed, although the numbers of differentially expressed transcripts were substantially smaller. Gender differences in mRNA and lncRNA expression profiles were also evident in non-failing human atria and ventricles. Gene ontology classification of differentially expressed gene sets revealed chamber-specific enrichment of numerous signaling pathways.
format	article
author	Eric K. Johnson Scot J. Matkovich Jeanne M. Nerbonne
author_facet	Eric K. Johnson Scot J. Matkovich Jeanne M. Nerbonne
author_sort	Eric K. Johnson
title	Regional Differences in mRNA and lncRNA Expression Profiles in Non-Failing Human Atria and Ventricles
title_short	Regional Differences in mRNA and lncRNA Expression Profiles in Non-Failing Human Atria and Ventricles
title_full	Regional Differences in mRNA and lncRNA Expression Profiles in Non-Failing Human Atria and Ventricles
title_fullStr	Regional Differences in mRNA and lncRNA Expression Profiles in Non-Failing Human Atria and Ventricles
title_full_unstemmed	Regional Differences in mRNA and lncRNA Expression Profiles in Non-Failing Human Atria and Ventricles
title_sort	regional differences in mrna and lncrna expression profiles in non-failing human atria and ventricles
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/677aef6ddd89489292f6b166481d1072
work_keys_str_mv	AT erickjohnson regionaldifferencesinmrnaandlncrnaexpressionprofilesinnonfailinghumanatriaandventricles AT scotjmatkovich regionaldifferencesinmrnaandlncrnaexpressionprofilesinnonfailinghumanatriaandventricles AT jeannemnerbonne regionaldifferencesinmrnaandlncrnaexpressionprofilesinnonfailinghumanatriaandventricles
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Regional Differences in mRNA and lncRNA Expression Profiles in Non-Failing Human Atria and Ventricles

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