Biosafety Level 3 setup for multiphoton microscopy in vivo

Abstract Multiphoton microscopy has revealed important insights into cellular behavior in vivo. However, its application in infectious settings often encounters technical, safety and regulatory limitations that prevent its wider use with highly virulent human pathogens. Herein, we present a method t...

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Autores principales:	D. Barlerin, G. Bessière, J. Domingues, M. Schuette, C. Feuillet, A. Peixoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/677b26d08e474fa7b841377f8d85bc21
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spelling	oai:doaj.org-article:677b26d08e474fa7b841377f8d85bc212021-12-02T15:05:38ZBiosafety Level 3 setup for multiphoton microscopy in vivo10.1038/s41598-017-00702-x2045-2322https://doaj.org/article/677b26d08e474fa7b841377f8d85bc212017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00702-xhttps://doaj.org/toc/2045-2322Abstract Multiphoton microscopy has revealed important insights into cellular behavior in vivo. However, its application in infectious settings often encounters technical, safety and regulatory limitations that prevent its wider use with highly virulent human pathogens. Herein, we present a method that renders multiphoton microscopy in vivo compatible with biosafety level 3 regulations and present an example of its application and potential to visualize a Mycobacterium tuberculosis infection of the mouse lung.D. BarlerinG. BessièreJ. DominguesM. SchuetteC. FeuilletA. PeixotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q D. Barlerin G. Bessière J. Domingues M. Schuette C. Feuillet A. Peixoto Biosafety Level 3 setup for multiphoton microscopy in vivo
description	Abstract Multiphoton microscopy has revealed important insights into cellular behavior in vivo. However, its application in infectious settings often encounters technical, safety and regulatory limitations that prevent its wider use with highly virulent human pathogens. Herein, we present a method that renders multiphoton microscopy in vivo compatible with biosafety level 3 regulations and present an example of its application and potential to visualize a Mycobacterium tuberculosis infection of the mouse lung.
format	article
author	D. Barlerin G. Bessière J. Domingues M. Schuette C. Feuillet A. Peixoto
author_facet	D. Barlerin G. Bessière J. Domingues M. Schuette C. Feuillet A. Peixoto
author_sort	D. Barlerin
title	Biosafety Level 3 setup for multiphoton microscopy in vivo
title_short	Biosafety Level 3 setup for multiphoton microscopy in vivo
title_full	Biosafety Level 3 setup for multiphoton microscopy in vivo
title_fullStr	Biosafety Level 3 setup for multiphoton microscopy in vivo
title_full_unstemmed	Biosafety Level 3 setup for multiphoton microscopy in vivo
title_sort	biosafety level 3 setup for multiphoton microscopy in vivo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/677b26d08e474fa7b841377f8d85bc21
work_keys_str_mv	AT dbarlerin biosafetylevel3setupformultiphotonmicroscopyinvivo AT gbessiere biosafetylevel3setupformultiphotonmicroscopyinvivo AT jdomingues biosafetylevel3setupformultiphotonmicroscopyinvivo AT mschuette biosafetylevel3setupformultiphotonmicroscopyinvivo AT cfeuillet biosafetylevel3setupformultiphotonmicroscopyinvivo AT apeixoto biosafetylevel3setupformultiphotonmicroscopyinvivo
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Biosafety Level 3 setup for multiphoton microscopy in vivo

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