Fluorescence Approaches for Characterizing Ion Channels in Synthetic Bilayers

Ion channels are membrane proteins that play important roles in a wide range of fundamental cellular processes. Studying membrane proteins at a molecular level becomes challenging in complex cellular environments. Instead, many studies focus on the isolation and reconstitution of the membrane protei...

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Autores principales:	Md. Sirajul Islam, James P. Gaston, Matthew A. B. Baker
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ion channel liposome synthetic bilayer membrane protein rhodopsins reconstitution Chemical technology TP1-1185 Chemical engineering TP155-156
Acceso en línea:	https://doaj.org/article/67939f2810604a75afde932287c93983
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spelling	oai:doaj.org-article:67939f2810604a75afde932287c939832021-11-25T18:19:51ZFluorescence Approaches for Characterizing Ion Channels in Synthetic Bilayers10.3390/membranes111108572077-0375https://doaj.org/article/67939f2810604a75afde932287c939832021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0375/11/11/857https://doaj.org/toc/2077-0375Ion channels are membrane proteins that play important roles in a wide range of fundamental cellular processes. Studying membrane proteins at a molecular level becomes challenging in complex cellular environments. Instead, many studies focus on the isolation and reconstitution of the membrane proteins into model lipid membranes. Such simpler, in vitro, systems offer the advantage of control over the membrane and protein composition and the lipid environment. Rhodopsin and rhodopsin-like ion channels are widely studied due to their light-interacting properties and are a natural candidate for investigation with fluorescence methods. Here we review techniques for synthesizing liposomes and for reconstituting membrane proteins into lipid bilayers. We then summarize fluorescence assays which can be used to verify the functionality of reconstituted membrane proteins in synthetic liposomes.Md. Sirajul IslamJames P. GastonMatthew A. B. BakerMDPI AGarticleion channelliposomesynthetic bilayermembrane proteinrhodopsinsreconstitutionChemical technologyTP1-1185Chemical engineeringTP155-156ENMembranes, Vol 11, Iss 857, p 857 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ion channel liposome synthetic bilayer membrane protein rhodopsins reconstitution Chemical technology TP1-1185 Chemical engineering TP155-156
spellingShingle	ion channel liposome synthetic bilayer membrane protein rhodopsins reconstitution Chemical technology TP1-1185 Chemical engineering TP155-156 Md. Sirajul Islam James P. Gaston Matthew A. B. Baker Fluorescence Approaches for Characterizing Ion Channels in Synthetic Bilayers
description	Ion channels are membrane proteins that play important roles in a wide range of fundamental cellular processes. Studying membrane proteins at a molecular level becomes challenging in complex cellular environments. Instead, many studies focus on the isolation and reconstitution of the membrane proteins into model lipid membranes. Such simpler, in vitro, systems offer the advantage of control over the membrane and protein composition and the lipid environment. Rhodopsin and rhodopsin-like ion channels are widely studied due to their light-interacting properties and are a natural candidate for investigation with fluorescence methods. Here we review techniques for synthesizing liposomes and for reconstituting membrane proteins into lipid bilayers. We then summarize fluorescence assays which can be used to verify the functionality of reconstituted membrane proteins in synthetic liposomes.
format	article
author	Md. Sirajul Islam James P. Gaston Matthew A. B. Baker
author_facet	Md. Sirajul Islam James P. Gaston Matthew A. B. Baker
author_sort	Md. Sirajul Islam
title	Fluorescence Approaches for Characterizing Ion Channels in Synthetic Bilayers
title_short	Fluorescence Approaches for Characterizing Ion Channels in Synthetic Bilayers
title_full	Fluorescence Approaches for Characterizing Ion Channels in Synthetic Bilayers
title_fullStr	Fluorescence Approaches for Characterizing Ion Channels in Synthetic Bilayers
title_full_unstemmed	Fluorescence Approaches for Characterizing Ion Channels in Synthetic Bilayers
title_sort	fluorescence approaches for characterizing ion channels in synthetic bilayers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/67939f2810604a75afde932287c93983
work_keys_str_mv	AT mdsirajulislam fluorescenceapproachesforcharacterizingionchannelsinsyntheticbilayers AT jamespgaston fluorescenceapproachesforcharacterizingionchannelsinsyntheticbilayers AT matthewabbaker fluorescenceapproachesforcharacterizingionchannelsinsyntheticbilayers
_version_	1718411323703820288

Fluorescence Approaches for Characterizing Ion Channels in Synthetic Bilayers

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