Direct protein quantification in complex sample solutions by surface-engineered nanorod probes

Abstract Detecting biomarkers from complex sample solutions is the key objective of molecular diagnostics. Being able to do so in a simple approach that does not require laborious sample preparation, sophisticated equipment and trained staff is vital for point-of-care applications. Here, we report o...

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Autores principales:	Stefan Schrittwieser, Beatriz Pelaz, Wolfgang J. Parak, Sergio Lentijo-Mozo, Katerina Soulantica, Jan Dieckhoff, Frank Ludwig, Joerg Schotter
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6793f75d1efd4175acfff301ea6d47a0
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spelling	oai:doaj.org-article:6793f75d1efd4175acfff301ea6d47a02021-12-02T16:06:27ZDirect protein quantification in complex sample solutions by surface-engineered nanorod probes10.1038/s41598-017-04970-52045-2322https://doaj.org/article/6793f75d1efd4175acfff301ea6d47a02017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04970-5https://doaj.org/toc/2045-2322Abstract Detecting biomarkers from complex sample solutions is the key objective of molecular diagnostics. Being able to do so in a simple approach that does not require laborious sample preparation, sophisticated equipment and trained staff is vital for point-of-care applications. Here, we report on the specific detection of the breast cancer biomarker sHER2 directly from serum and saliva samples by a nanorod-based homogeneous biosensing approach, which is easy to operate as it only requires mixing of the samples with the nanorod probes. By careful nanorod surface engineering and homogeneous assay design, we demonstrate that the formation of a protein corona around the nanoparticles does not limit the applicability of our detection method, but on the contrary enables us to conduct in-situ reference measurements, thus further strengthening the point-of-care applicability of our method. Making use of sandwich assays on top of the nanorods, we obtain a limit of detection of 110 pM and 470 pM in 10-fold diluted spiked saliva and serum samples, respectively. In conclusion, our results open up numerous applications in direct protein biomarker quantification, specifically in point-of-care settings where resources are limited and ease-of-use is of essence.Stefan SchrittwieserBeatriz PelazWolfgang J. ParakSergio Lentijo-MozoKaterina SoulanticaJan DieckhoffFrank LudwigJoerg SchotterNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefan Schrittwieser Beatriz Pelaz Wolfgang J. Parak Sergio Lentijo-Mozo Katerina Soulantica Jan Dieckhoff Frank Ludwig Joerg Schotter Direct protein quantification in complex sample solutions by surface-engineered nanorod probes
description	Abstract Detecting biomarkers from complex sample solutions is the key objective of molecular diagnostics. Being able to do so in a simple approach that does not require laborious sample preparation, sophisticated equipment and trained staff is vital for point-of-care applications. Here, we report on the specific detection of the breast cancer biomarker sHER2 directly from serum and saliva samples by a nanorod-based homogeneous biosensing approach, which is easy to operate as it only requires mixing of the samples with the nanorod probes. By careful nanorod surface engineering and homogeneous assay design, we demonstrate that the formation of a protein corona around the nanoparticles does not limit the applicability of our detection method, but on the contrary enables us to conduct in-situ reference measurements, thus further strengthening the point-of-care applicability of our method. Making use of sandwich assays on top of the nanorods, we obtain a limit of detection of 110 pM and 470 pM in 10-fold diluted spiked saliva and serum samples, respectively. In conclusion, our results open up numerous applications in direct protein biomarker quantification, specifically in point-of-care settings where resources are limited and ease-of-use is of essence.
format	article
author	Stefan Schrittwieser Beatriz Pelaz Wolfgang J. Parak Sergio Lentijo-Mozo Katerina Soulantica Jan Dieckhoff Frank Ludwig Joerg Schotter
author_facet	Stefan Schrittwieser Beatriz Pelaz Wolfgang J. Parak Sergio Lentijo-Mozo Katerina Soulantica Jan Dieckhoff Frank Ludwig Joerg Schotter
author_sort	Stefan Schrittwieser
title	Direct protein quantification in complex sample solutions by surface-engineered nanorod probes
title_short	Direct protein quantification in complex sample solutions by surface-engineered nanorod probes
title_full	Direct protein quantification in complex sample solutions by surface-engineered nanorod probes
title_fullStr	Direct protein quantification in complex sample solutions by surface-engineered nanorod probes
title_full_unstemmed	Direct protein quantification in complex sample solutions by surface-engineered nanorod probes
title_sort	direct protein quantification in complex sample solutions by surface-engineered nanorod probes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6793f75d1efd4175acfff301ea6d47a0
work_keys_str_mv	AT stefanschrittwieser directproteinquantificationincomplexsamplesolutionsbysurfaceengineerednanorodprobes AT beatrizpelaz directproteinquantificationincomplexsamplesolutionsbysurfaceengineerednanorodprobes AT wolfgangjparak directproteinquantificationincomplexsamplesolutionsbysurfaceengineerednanorodprobes AT sergiolentijomozo directproteinquantificationincomplexsamplesolutionsbysurfaceengineerednanorodprobes AT katerinasoulantica directproteinquantificationincomplexsamplesolutionsbysurfaceengineerednanorodprobes AT jandieckhoff directproteinquantificationincomplexsamplesolutionsbysurfaceengineerednanorodprobes AT frankludwig directproteinquantificationincomplexsamplesolutionsbysurfaceengineerednanorodprobes AT joergschotter directproteinquantificationincomplexsamplesolutionsbysurfaceengineerednanorodprobes
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Direct protein quantification in complex sample solutions by surface-engineered nanorod probes

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