Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica).

The Australian finger lime is a unique citrus species that has gained importance due to its unique fruit characteristics and perceived tolerance to Huanglongbing (HLB), an often-fatal disease of citrus trees. In this study, we developed allotetraploid finger lime hybrids and cybrids by utilizing som...

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Autores principales:	Manjul Dutt, Lamiaa M Mahmoud, Karen Chamusco, Daniel Stanton, Christine D Chase, Ethan Nielsen, Maria Quirico, Qibin Yu, Frederick G Gmitter, Jude W Grosser
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6796236fd6154277822208737d17de92
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spelling	oai:doaj.org-article:6796236fd6154277822208737d17de922021-12-02T20:18:28ZUtilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica).1932-620310.1371/journal.pone.0255842https://doaj.org/article/6796236fd6154277822208737d17de922021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255842https://doaj.org/toc/1932-6203The Australian finger lime is a unique citrus species that has gained importance due to its unique fruit characteristics and perceived tolerance to Huanglongbing (HLB), an often-fatal disease of citrus trees. In this study, we developed allotetraploid finger lime hybrids and cybrids by utilizing somatic cell fusion techniques to fuse diploid 'OLL8' sweet orange or 'Page' tangelo callus-derived protoplasts with finger lime (FL) mesophyll-derived protoplasts. Six somatic fusions were regenerated from the 'OLL8' + FL fusion, while three putative cybrids were regenerated from the 'Page' + FL fusion. Ploidy levels and nuclear-expressed sequence tag derived simple sequence repeat (EST-SSR) markers confirmed the somatic hybrid production, and mitochondrial DNA primer sets confirmed the cybrid nature. Several trees produced by the somatic fusion remained HLB negative even after 6 years of growth in an HLB-endemic environment. Pathogenesis related (PR) and other genes that are often upregulated in HLB-tolerant trees were also upregulated in our somatic fusions. These newly developed somatic fusions and cybrids could potentially be used as breeding parents to develop the next generation of improved HLB-tolerant rootstocks and scions.Manjul DuttLamiaa M MahmoudKaren ChamuscoDaniel StantonChristine D ChaseEthan NielsenMaria QuiricoQibin YuFrederick G GmitterJude W GrosserPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255842 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manjul Dutt Lamiaa M Mahmoud Karen Chamusco Daniel Stanton Christine D Chase Ethan Nielsen Maria Quirico Qibin Yu Frederick G Gmitter Jude W Grosser Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica).
description	The Australian finger lime is a unique citrus species that has gained importance due to its unique fruit characteristics and perceived tolerance to Huanglongbing (HLB), an often-fatal disease of citrus trees. In this study, we developed allotetraploid finger lime hybrids and cybrids by utilizing somatic cell fusion techniques to fuse diploid 'OLL8' sweet orange or 'Page' tangelo callus-derived protoplasts with finger lime (FL) mesophyll-derived protoplasts. Six somatic fusions were regenerated from the 'OLL8' + FL fusion, while three putative cybrids were regenerated from the 'Page' + FL fusion. Ploidy levels and nuclear-expressed sequence tag derived simple sequence repeat (EST-SSR) markers confirmed the somatic hybrid production, and mitochondrial DNA primer sets confirmed the cybrid nature. Several trees produced by the somatic fusion remained HLB negative even after 6 years of growth in an HLB-endemic environment. Pathogenesis related (PR) and other genes that are often upregulated in HLB-tolerant trees were also upregulated in our somatic fusions. These newly developed somatic fusions and cybrids could potentially be used as breeding parents to develop the next generation of improved HLB-tolerant rootstocks and scions.
format	article
author	Manjul Dutt Lamiaa M Mahmoud Karen Chamusco Daniel Stanton Christine D Chase Ethan Nielsen Maria Quirico Qibin Yu Frederick G Gmitter Jude W Grosser
author_facet	Manjul Dutt Lamiaa M Mahmoud Karen Chamusco Daniel Stanton Christine D Chase Ethan Nielsen Maria Quirico Qibin Yu Frederick G Gmitter Jude W Grosser
author_sort	Manjul Dutt
title	Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica).
title_short	Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica).
title_full	Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica).
title_fullStr	Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica).
title_full_unstemmed	Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica).
title_sort	utilization of somatic fusion techniques for the development of hlb tolerant breeding resources employing the australian finger lime (citrus australasica).
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6796236fd6154277822208737d17de92
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Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica).

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