Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats

Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocyte...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Danilović Luković Jelena, Korać Aleksandra, Milošević Ivan, Lužajić Tijana, Milanović Zorana, Kovačević Filipović Milica, Radovanović Anita
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	atg7 caspase-3 lc3 meiosis prophase i secondary necrosis Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/6796683b782542a3b7e4e22c6b101355
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6796683b782542a3b7e4e22c6b101355
record_format	dspace
spelling	oai:doaj.org-article:6796683b782542a3b7e4e22c6b1013552021-11-17T21:27:51ZZ-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats1820-744810.1515/acve-2017-0009https://doaj.org/article/6796683b782542a3b7e4e22c6b1013552017-03-01T00:00:00Zhttps://doi.org/10.1515/acve-2017-0009https://doaj.org/toc/1820-7448Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/oocytes altered with the advanced process of autophagy.Danilović Luković JelenaKorać AleksandraMilošević IvanLužajić TijanaMilanović ZoranaKovačević Filipović MilicaRadovanović AnitaSciendoarticleatg7caspase-3lc3meiosis prophase isecondary necrosisVeterinary medicineSF600-1100ENActa Veterinaria, Vol 67, Iss 1, Pp 92-106 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	atg7 caspase-3 lc3 meiosis prophase i secondary necrosis Veterinary medicine SF600-1100
spellingShingle	atg7 caspase-3 lc3 meiosis prophase i secondary necrosis Veterinary medicine SF600-1100 Danilović Luković Jelena Korać Aleksandra Milošević Ivan Lužajić Tijana Milanović Zorana Kovačević Filipović Milica Radovanović Anita Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
description	Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/oocytes altered with the advanced process of autophagy.
format	article
author	Danilović Luković Jelena Korać Aleksandra Milošević Ivan Lužajić Tijana Milanović Zorana Kovačević Filipović Milica Radovanović Anita
author_facet	Danilović Luković Jelena Korać Aleksandra Milošević Ivan Lužajić Tijana Milanović Zorana Kovačević Filipović Milica Radovanović Anita
author_sort	Danilović Luković Jelena
title	Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
title_short	Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
title_full	Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
title_fullStr	Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
title_full_unstemmed	Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
title_sort	z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/6796683b782542a3b7e4e22c6b101355
work_keys_str_mv	AT daniloviclukovicjelena zcellsandoogoniaoocytesintheadvancedprocessofautophagyarethedominantalteredcellsintheovariesofhypothyroidnewbornrats AT koracaleksandra zcellsandoogoniaoocytesintheadvancedprocessofautophagyarethedominantalteredcellsintheovariesofhypothyroidnewbornrats AT milosevicivan zcellsandoogoniaoocytesintheadvancedprocessofautophagyarethedominantalteredcellsintheovariesofhypothyroidnewbornrats AT luzajictijana zcellsandoogoniaoocytesintheadvancedprocessofautophagyarethedominantalteredcellsintheovariesofhypothyroidnewbornrats AT milanoviczorana zcellsandoogoniaoocytesintheadvancedprocessofautophagyarethedominantalteredcellsintheovariesofhypothyroidnewbornrats AT kovacevicfilipovicmilica zcellsandoogoniaoocytesintheadvancedprocessofautophagyarethedominantalteredcellsintheovariesofhypothyroidnewbornrats AT radovanovicanita zcellsandoogoniaoocytesintheadvancedprocessofautophagyarethedominantalteredcellsintheovariesofhypothyroidnewbornrats
_version_	1718425287027326976

Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats

Ejemplares similares