Development of a gold nanoparticle immunochromatographic assay for the on-site analysis of 6-benzylaminopurine residues in bean sprouts

The presence of pesticide residues in crops is a significant public concern. Due to the large number of samples and pesticides, a reliable, simple, cost-effective, and high-throughput analytic method is required. In this study, we developed a gold nanoparticle (GNP) immunochromatographic assay (ICA)...

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Autores principales:	Yue Li, Liqiang Liu, Shanshan Song, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	immunochromatographic assay 6-benzylaminopurine monoclonal antibody bean sprouts Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/679996865fed4ea784637dd2e715479f
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spelling	oai:doaj.org-article:679996865fed4ea784637dd2e715479f2021-11-26T11:19:46ZDevelopment of a gold nanoparticle immunochromatographic assay for the on-site analysis of 6-benzylaminopurine residues in bean sprouts0954-01051465-344310.1080/09540105.2017.1354359https://doaj.org/article/679996865fed4ea784637dd2e715479f2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1354359https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443The presence of pesticide residues in crops is a significant public concern. Due to the large number of samples and pesticides, a reliable, simple, cost-effective, and high-throughput analytic method is required. In this study, we developed a gold nanoparticle (GNP) immunochromatographic assay (ICA) for the rapid detection of 6-benzylaminopurine (6-BA) residues in bean sprouts. A monoclonal antibody against 6-BA, having a half-maximum inhibition concentration of 2.25 ng/mL, was labeled with GNPs and fixed onto a glass fiber membrane to create a conjugate pad. Coating antigen and goat anti-mouse antibody were sprayed onto a nitrocellulose membrane to form the test line and control line, respectively. The visual limit of detection and cutoff limit of ICA were 10 and 80 ng/g in bean sprouts, respectively. The results of ICA were obtained within 10 min with the naked eye. Therefore, ICA is a rapid tool for the on-site screening and detection of 6-BA in bean sprouts.Yue LiLiqiang LiuShanshan SongHua KuangTaylor & Francis Grouparticleimmunochromatographic assay6-benzylaminopurinemonoclonal antibodybean sproutsAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 14-26 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	immunochromatographic assay 6-benzylaminopurine monoclonal antibody bean sprouts Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	immunochromatographic assay 6-benzylaminopurine monoclonal antibody bean sprouts Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Yue Li Liqiang Liu Shanshan Song Hua Kuang Development of a gold nanoparticle immunochromatographic assay for the on-site analysis of 6-benzylaminopurine residues in bean sprouts
description	The presence of pesticide residues in crops is a significant public concern. Due to the large number of samples and pesticides, a reliable, simple, cost-effective, and high-throughput analytic method is required. In this study, we developed a gold nanoparticle (GNP) immunochromatographic assay (ICA) for the rapid detection of 6-benzylaminopurine (6-BA) residues in bean sprouts. A monoclonal antibody against 6-BA, having a half-maximum inhibition concentration of 2.25 ng/mL, was labeled with GNPs and fixed onto a glass fiber membrane to create a conjugate pad. Coating antigen and goat anti-mouse antibody were sprayed onto a nitrocellulose membrane to form the test line and control line, respectively. The visual limit of detection and cutoff limit of ICA were 10 and 80 ng/g in bean sprouts, respectively. The results of ICA were obtained within 10 min with the naked eye. Therefore, ICA is a rapid tool for the on-site screening and detection of 6-BA in bean sprouts.
format	article
author	Yue Li Liqiang Liu Shanshan Song Hua Kuang
author_facet	Yue Li Liqiang Liu Shanshan Song Hua Kuang
author_sort	Yue Li
title	Development of a gold nanoparticle immunochromatographic assay for the on-site analysis of 6-benzylaminopurine residues in bean sprouts
title_short	Development of a gold nanoparticle immunochromatographic assay for the on-site analysis of 6-benzylaminopurine residues in bean sprouts
title_full	Development of a gold nanoparticle immunochromatographic assay for the on-site analysis of 6-benzylaminopurine residues in bean sprouts
title_fullStr	Development of a gold nanoparticle immunochromatographic assay for the on-site analysis of 6-benzylaminopurine residues in bean sprouts
title_full_unstemmed	Development of a gold nanoparticle immunochromatographic assay for the on-site analysis of 6-benzylaminopurine residues in bean sprouts
title_sort	development of a gold nanoparticle immunochromatographic assay for the on-site analysis of 6-benzylaminopurine residues in bean sprouts
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/679996865fed4ea784637dd2e715479f
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Development of a gold nanoparticle immunochromatographic assay for the on-site analysis of 6-benzylaminopurine residues in bean sprouts

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