MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells

Abstract Natriuretic Peptide Receptor 3 (NPR3), the clearance receptor for extracellular bio-active natriuretic peptides (NPs), plays important roles in the homeostasis of body fluid volume and vascular tone. Using luciferase reporter and antagomir-based silencing assays, we demonstrated that the ex...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Juan Wang, Kai Sing Tong, Lee Lee Wong, Oi-Wah Liew, Divya Raghuram, Arthur Mark Richards, Yei-Tsung Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/67a2a64447b84e3bb8fff82915af503b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:67a2a64447b84e3bb8fff82915af503b
record_format	dspace
spelling	oai:doaj.org-article:67a2a64447b84e3bb8fff82915af503b2021-12-02T12:32:47ZMicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells10.1038/s41598-018-25489-32045-2322https://doaj.org/article/67a2a64447b84e3bb8fff82915af503b2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25489-3https://doaj.org/toc/2045-2322Abstract Natriuretic Peptide Receptor 3 (NPR3), the clearance receptor for extracellular bio-active natriuretic peptides (NPs), plays important roles in the homeostasis of body fluid volume and vascular tone. Using luciferase reporter and antagomir-based silencing assays, we demonstrated that the expression of NPR3 could be modulated by microRNA-143 (miR-143-3p), a microRNA species with up-regulated circulating concentrations in clinical heart failure. The regulatory effect of miR-143 on NPR3 expression was further evidenced by the reciprocal relationship between miR-143 and NPR3 levels observed in hypoxia-treated human cardiac cells and in left ventricular tissue from rats undergoing experimental myocardial infarction. Further analysis indicated elevation of miR-143 in response to hypoxic challenge reflects transcriptional activation of the miR-143 host gene (MIR143HG). This was corroborated by demonstration of the induction of host gene promoter activity upon hypoxic challenge. Moreover, miR-143 was shown to enhance its own expression by increasing MIR143HG promoter activity, as well as targeting the expressions of NPPA, NPPC, NR3C2, and CRHR2 in cardiac cells. Taken together, these findings suggest that the elevation of miR-143 upon hypoxic insult may be part of a microRNA-based feed forward loop that results in fine tuning the levels of NPs and neurohormonal receptors in cardiac cell lineages.Juan WangKai Sing TongLee Lee WongOi-Wah LiewDivya RaghuramArthur Mark RichardsYei-Tsung ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Juan Wang Kai Sing Tong Lee Lee Wong Oi-Wah Liew Divya Raghuram Arthur Mark Richards Yei-Tsung Chen MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells
description	Abstract Natriuretic Peptide Receptor 3 (NPR3), the clearance receptor for extracellular bio-active natriuretic peptides (NPs), plays important roles in the homeostasis of body fluid volume and vascular tone. Using luciferase reporter and antagomir-based silencing assays, we demonstrated that the expression of NPR3 could be modulated by microRNA-143 (miR-143-3p), a microRNA species with up-regulated circulating concentrations in clinical heart failure. The regulatory effect of miR-143 on NPR3 expression was further evidenced by the reciprocal relationship between miR-143 and NPR3 levels observed in hypoxia-treated human cardiac cells and in left ventricular tissue from rats undergoing experimental myocardial infarction. Further analysis indicated elevation of miR-143 in response to hypoxic challenge reflects transcriptional activation of the miR-143 host gene (MIR143HG). This was corroborated by demonstration of the induction of host gene promoter activity upon hypoxic challenge. Moreover, miR-143 was shown to enhance its own expression by increasing MIR143HG promoter activity, as well as targeting the expressions of NPPA, NPPC, NR3C2, and CRHR2 in cardiac cells. Taken together, these findings suggest that the elevation of miR-143 upon hypoxic insult may be part of a microRNA-based feed forward loop that results in fine tuning the levels of NPs and neurohormonal receptors in cardiac cell lineages.
format	article
author	Juan Wang Kai Sing Tong Lee Lee Wong Oi-Wah Liew Divya Raghuram Arthur Mark Richards Yei-Tsung Chen
author_facet	Juan Wang Kai Sing Tong Lee Lee Wong Oi-Wah Liew Divya Raghuram Arthur Mark Richards Yei-Tsung Chen
author_sort	Juan Wang
title	MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells
title_short	MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells
title_full	MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells
title_fullStr	MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells
title_full_unstemmed	MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells
title_sort	microrna-143 modulates the expression of natriuretic peptide receptor 3 in cardiac cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/67a2a64447b84e3bb8fff82915af503b
work_keys_str_mv	AT juanwang microrna143modulatestheexpressionofnatriureticpeptidereceptor3incardiaccells AT kaisingtong microrna143modulatestheexpressionofnatriureticpeptidereceptor3incardiaccells AT leeleewong microrna143modulatestheexpressionofnatriureticpeptidereceptor3incardiaccells AT oiwahliew microrna143modulatestheexpressionofnatriureticpeptidereceptor3incardiaccells AT divyaraghuram microrna143modulatestheexpressionofnatriureticpeptidereceptor3incardiaccells AT arthurmarkrichards microrna143modulatestheexpressionofnatriureticpeptidereceptor3incardiaccells AT yeitsungchen microrna143modulatestheexpressionofnatriureticpeptidereceptor3incardiaccells
_version_	1718393982771265536

MicroRNA-143 modulates the expression of Natriuretic Peptide Receptor 3 in cardiac cells

Ejemplares similares