Genetic circuit characterization by inferring RNA polymerase movement and ribosome usage

Debugging a genetic circuit is frustrated by the inability to characterize parts in the context of the circuit. Here the authors use RNA-seq and ribosome profiling to take ‘snapshots’ of a large circuit in different states.

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Detalles Bibliográficos
Autores principales: Amin Espah Borujeni, Jing Zhang, Hamid Doosthosseini, Alec A. K. Nielsen, Christopher A. Voigt
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/67a63b86a841423c9a18630376a49a5c
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Descripción
Sumario:Debugging a genetic circuit is frustrated by the inability to characterize parts in the context of the circuit. Here the authors use RNA-seq and ribosome profiling to take ‘snapshots’ of a large circuit in different states.