Snow surface microbiome on the High Antarctic Plateau (DOME C).
The cryosphere is an integral part of the global climate system and one of the major habitable ecosystems of Earth's biosphere. These permanently frozen environments harbor diverse, viable and metabolically active microbial populations that represent almost all the major phylogenetic groups. In...
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2014
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oai:doaj.org-article:67a8ed0a955f48e7a040956a60bd9ffa2021-11-25T06:05:40ZSnow surface microbiome on the High Antarctic Plateau (DOME C).1932-620310.1371/journal.pone.0104505https://doaj.org/article/67a8ed0a955f48e7a040956a60bd9ffa2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25101779/?tool=EBIhttps://doaj.org/toc/1932-6203The cryosphere is an integral part of the global climate system and one of the major habitable ecosystems of Earth's biosphere. These permanently frozen environments harbor diverse, viable and metabolically active microbial populations that represent almost all the major phylogenetic groups. In this study, we investigated the microbial diversity in the surface snow surrounding the Concordia Research Station on the High Antarctic Plateau through a polyphasic approach, including direct prokaryotic quantification by flow cytometry and catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), and phylogenetic identification by 16S RNA gene clone library sequencing and 454 16S amplicon pyrosequencing. Although the microbial abundance was low (<10(3) cells/ml of snowmelt), concordant results were obtained with the different techniques. The microbial community was mainly composed of members of the Alpha-proteobacteria class (e.g. Kiloniellaceae and Rhodobacteraceae), which is one of the most well-represented bacterial groups in marine habitats, Bacteroidetes (e.g. Cryomorphaceae and Flavobacteriaceae) and Cyanobacteria. Based on our results, polar microorganisms could not only be considered as deposited airborne particles, but as an active component of the snowpack ecology of the High Antarctic Plateau.Luigi MichaudAngelina Lo GiudiceMohamed MysaraPieter MonsieursCarmela RaffaNatalie LeysStefano AmalfitanoRob Van HoudtPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e104505 (2014) |
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DOAJ |
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| topic |
Medicine R Science Q |
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Medicine R Science Q Luigi Michaud Angelina Lo Giudice Mohamed Mysara Pieter Monsieurs Carmela Raffa Natalie Leys Stefano Amalfitano Rob Van Houdt Snow surface microbiome on the High Antarctic Plateau (DOME C). |
| description |
The cryosphere is an integral part of the global climate system and one of the major habitable ecosystems of Earth's biosphere. These permanently frozen environments harbor diverse, viable and metabolically active microbial populations that represent almost all the major phylogenetic groups. In this study, we investigated the microbial diversity in the surface snow surrounding the Concordia Research Station on the High Antarctic Plateau through a polyphasic approach, including direct prokaryotic quantification by flow cytometry and catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), and phylogenetic identification by 16S RNA gene clone library sequencing and 454 16S amplicon pyrosequencing. Although the microbial abundance was low (<10(3) cells/ml of snowmelt), concordant results were obtained with the different techniques. The microbial community was mainly composed of members of the Alpha-proteobacteria class (e.g. Kiloniellaceae and Rhodobacteraceae), which is one of the most well-represented bacterial groups in marine habitats, Bacteroidetes (e.g. Cryomorphaceae and Flavobacteriaceae) and Cyanobacteria. Based on our results, polar microorganisms could not only be considered as deposited airborne particles, but as an active component of the snowpack ecology of the High Antarctic Plateau. |
| format |
article |
| author |
Luigi Michaud Angelina Lo Giudice Mohamed Mysara Pieter Monsieurs Carmela Raffa Natalie Leys Stefano Amalfitano Rob Van Houdt |
| author_facet |
Luigi Michaud Angelina Lo Giudice Mohamed Mysara Pieter Monsieurs Carmela Raffa Natalie Leys Stefano Amalfitano Rob Van Houdt |
| author_sort |
Luigi Michaud |
| title |
Snow surface microbiome on the High Antarctic Plateau (DOME C). |
| title_short |
Snow surface microbiome on the High Antarctic Plateau (DOME C). |
| title_full |
Snow surface microbiome on the High Antarctic Plateau (DOME C). |
| title_fullStr |
Snow surface microbiome on the High Antarctic Plateau (DOME C). |
| title_full_unstemmed |
Snow surface microbiome on the High Antarctic Plateau (DOME C). |
| title_sort |
snow surface microbiome on the high antarctic plateau (dome c). |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/67a8ed0a955f48e7a040956a60bd9ffa |
| work_keys_str_mv |
AT luigimichaud snowsurfacemicrobiomeonthehighantarcticplateaudomec AT angelinalogiudice snowsurfacemicrobiomeonthehighantarcticplateaudomec AT mohamedmysara snowsurfacemicrobiomeonthehighantarcticplateaudomec AT pietermonsieurs snowsurfacemicrobiomeonthehighantarcticplateaudomec AT carmelaraffa snowsurfacemicrobiomeonthehighantarcticplateaudomec AT natalieleys snowsurfacemicrobiomeonthehighantarcticplateaudomec AT stefanoamalfitano snowsurfacemicrobiomeonthehighantarcticplateaudomec AT robvanhoudt snowsurfacemicrobiomeonthehighantarcticplateaudomec |
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