Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles.

Bacteria use a variety of secreted virulence factors to manipulate host cells, thereby causing significant morbidity and mortality. We report a mechanism for the long-distance delivery of multiple bacterial virulence factors, simultaneously and directly into the host cell cytoplasm, thus obviating t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jennifer M Bomberger, Daniel P Maceachran, Bonita A Coutermarsh, Siying Ye, George A O'Toole, Bruce A Stanton
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/67ab7df7c9524f4a951acb09a566686f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:67ab7df7c9524f4a951acb09a566686f
record_format	dspace
spelling	oai:doaj.org-article:67ab7df7c9524f4a951acb09a566686f2021-11-25T05:47:07ZLong-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles.1553-73661553-737410.1371/journal.ppat.1000382https://doaj.org/article/67ab7df7c9524f4a951acb09a566686f2009-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19360133/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Bacteria use a variety of secreted virulence factors to manipulate host cells, thereby causing significant morbidity and mortality. We report a mechanism for the long-distance delivery of multiple bacterial virulence factors, simultaneously and directly into the host cell cytoplasm, thus obviating the need for direct interaction of the pathogen with the host cell to cause cytotoxicity. We show that outer membrane-derived vesicles (OMV) secreted by the opportunistic human pathogen Pseudomonas aeruginosa deliver multiple virulence factors, including beta-lactamase, alkaline phosphatase, hemolytic phospholipase C, and Cif, directly into the host cytoplasm via fusion of OMV with lipid rafts in the host plasma membrane. These virulence factors enter the cytoplasm of the host cell via N-WASP-mediated actin trafficking, where they rapidly distribute to specific subcellular locations to affect host cell biology. We propose that secreted virulence factors are not released individually as naked proteins into the surrounding milieu where they may randomly contact the surface of the host cell, but instead bacterial derived OMV deliver multiple virulence factors simultaneously and directly into the host cell cytoplasm in a coordinated manner.Jennifer M BombergerDaniel P MaceachranBonita A CoutermarshSiying YeGeorge A O'TooleBruce A StantonPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 4, p e1000382 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Jennifer M Bomberger Daniel P Maceachran Bonita A Coutermarsh Siying Ye George A O'Toole Bruce A Stanton Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles.
description	Bacteria use a variety of secreted virulence factors to manipulate host cells, thereby causing significant morbidity and mortality. We report a mechanism for the long-distance delivery of multiple bacterial virulence factors, simultaneously and directly into the host cell cytoplasm, thus obviating the need for direct interaction of the pathogen with the host cell to cause cytotoxicity. We show that outer membrane-derived vesicles (OMV) secreted by the opportunistic human pathogen Pseudomonas aeruginosa deliver multiple virulence factors, including beta-lactamase, alkaline phosphatase, hemolytic phospholipase C, and Cif, directly into the host cytoplasm via fusion of OMV with lipid rafts in the host plasma membrane. These virulence factors enter the cytoplasm of the host cell via N-WASP-mediated actin trafficking, where they rapidly distribute to specific subcellular locations to affect host cell biology. We propose that secreted virulence factors are not released individually as naked proteins into the surrounding milieu where they may randomly contact the surface of the host cell, but instead bacterial derived OMV deliver multiple virulence factors simultaneously and directly into the host cell cytoplasm in a coordinated manner.
format	article
author	Jennifer M Bomberger Daniel P Maceachran Bonita A Coutermarsh Siying Ye George A O'Toole Bruce A Stanton
author_facet	Jennifer M Bomberger Daniel P Maceachran Bonita A Coutermarsh Siying Ye George A O'Toole Bruce A Stanton
author_sort	Jennifer M Bomberger
title	Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles.
title_short	Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles.
title_full	Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles.
title_fullStr	Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles.
title_full_unstemmed	Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles.
title_sort	long-distance delivery of bacterial virulence factors by pseudomonas aeruginosa outer membrane vesicles.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/67ab7df7c9524f4a951acb09a566686f
work_keys_str_mv	AT jennifermbomberger longdistancedeliveryofbacterialvirulencefactorsbypseudomonasaeruginosaoutermembranevesicles AT danielpmaceachran longdistancedeliveryofbacterialvirulencefactorsbypseudomonasaeruginosaoutermembranevesicles AT bonitaacoutermarsh longdistancedeliveryofbacterialvirulencefactorsbypseudomonasaeruginosaoutermembranevesicles AT siyingye longdistancedeliveryofbacterialvirulencefactorsbypseudomonasaeruginosaoutermembranevesicles AT georgeaotoole longdistancedeliveryofbacterialvirulencefactorsbypseudomonasaeruginosaoutermembranevesicles AT bruceastanton longdistancedeliveryofbacterialvirulencefactorsbypseudomonasaeruginosaoutermembranevesicles
_version_	1718414459990441984

Long-distance delivery of bacterial virulence factors by Pseudomonas aeruginosa outer membrane vesicles.

Ejemplares similares