Studying Humoral Immune Response at Mild and Asymptomatic COVID-19 Forms

Background. Currently, during the COVID-19 pandemic, one of the most pressing healthcare problems is to ensure the safety of the population of the Russian Federation. There is insufficient information on the duration and intensity of post-infectious immunity in relation to SARS-CoV-2, especially in...

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Autores principales:	S. V. Balakhonov, V. I. Dubrovina, M. V. Chesnokova, V. V. Voitkova, A. B. Pyatidesyatnikova, D. D. Bryukhova, N. O. Kiseleva, K. M. Korytov, T. G. Kuznetsova, Zh. V. Markevich
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2020
Materias:	humoral immunity specific antibodies covid-19 sars-cov-2 Science Q
Acceso en línea:	https://doaj.org/article/67b0c32d84344077850233a511edb718
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spelling	oai:doaj.org-article:67b0c32d84344077850233a511edb7182021-11-23T06:14:44ZStudying Humoral Immune Response at Mild and Asymptomatic COVID-19 Forms2541-94202587-959610.29413/ABS.2020-5.5.3https://doaj.org/article/67b0c32d84344077850233a511edb7182020-11-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2436https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. Currently, during the COVID-19 pandemic, one of the most pressing healthcare problems is to ensure the safety of the population of the Russian Federation. There is insufficient information on the duration and intensity of post-infectious immunity in relation to SARS-CoV-2, especially in case of inapparent (without clinical signs), mild or latent forms of infection.Materials and methods. The study involved 99 volunteers with a laboratory confirmed PCR diagnosis of COVID-19 who were residents of the Irkutsk Region and of other regions of the Russian Federation and arrived to work in Bodaybo on a rotational basis. Also the study included 50 conditionally healthy people living in Irkutsk who had negative PCR results for SARS-CoV-2 RNA presence.Specific IgG and IgM antibodies to SARS-CoV-2 were detected in blood serum by enzyme-linked immunosorbent assay (ELISA) using test systems “ELISA anti-SARS-CoV-2 IgG” (FBUN SSC PMB, Obolensk, Russian Federation), “SARS-CoV-2-IgG-IFA-BEST” and “SARS-CoV-2-IgM-IFA-BEST” (Vector-Best, Novosibirsk, Russian Federation).Results. The results of a study of the humoral immunity of patients with asymptomatic and clinical forms of COVID-19 are presented. The data indicate the production of specific IgG in the blood serum of people in 2–3 weeks after SARSCov-2 infection and reaching its maximum level on the 20–21st day. The seroconversion rate was 94.9 %. It was shown that the geometric mean titer of antibodies in asymptomatic and mild forms of coronavirus infection did not differ statistically and amounted to 1:512 and 1:632, respectively. Higher titers of antibodies (1:1600) were detected in the moderate form.Conclusion. The research results can serve as a basis for studying the dynamics of changes in the indicators of the humoral immune response in patients with COVID-19 and for clarifying the duration of their post- infectious immunity in order to predict the development of the epidemic situation and to ensure the planning of specific prevention.S. V. BalakhonovV. I. DubrovinaM. V. ChesnokovaV. V. VoitkovaA. B. PyatidesyatnikovaD. D. BryukhovaN. O. KiselevaK. M. KorytovT. G. KuznetsovaZh. V. MarkevichScientific Сentre for Family Health and Human Reproduction Problemsarticlehumoral immunityspecific antibodiescovid-19sars-cov-2ScienceQRUActa Biomedica Scientifica, Vol 5, Iss 5, Pp 26-30 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	humoral immunity specific antibodies covid-19 sars-cov-2 Science Q
spellingShingle	humoral immunity specific antibodies covid-19 sars-cov-2 Science Q S. V. Balakhonov V. I. Dubrovina M. V. Chesnokova V. V. Voitkova A. B. Pyatidesyatnikova D. D. Bryukhova N. O. Kiseleva K. M. Korytov T. G. Kuznetsova Zh. V. Markevich Studying Humoral Immune Response at Mild and Asymptomatic COVID-19 Forms
description	Background. Currently, during the COVID-19 pandemic, one of the most pressing healthcare problems is to ensure the safety of the population of the Russian Federation. There is insufficient information on the duration and intensity of post-infectious immunity in relation to SARS-CoV-2, especially in case of inapparent (without clinical signs), mild or latent forms of infection.Materials and methods. The study involved 99 volunteers with a laboratory confirmed PCR diagnosis of COVID-19 who were residents of the Irkutsk Region and of other regions of the Russian Federation and arrived to work in Bodaybo on a rotational basis. Also the study included 50 conditionally healthy people living in Irkutsk who had negative PCR results for SARS-CoV-2 RNA presence.Specific IgG and IgM antibodies to SARS-CoV-2 were detected in blood serum by enzyme-linked immunosorbent assay (ELISA) using test systems “ELISA anti-SARS-CoV-2 IgG” (FBUN SSC PMB, Obolensk, Russian Federation), “SARS-CoV-2-IgG-IFA-BEST” and “SARS-CoV-2-IgM-IFA-BEST” (Vector-Best, Novosibirsk, Russian Federation).Results. The results of a study of the humoral immunity of patients with asymptomatic and clinical forms of COVID-19 are presented. The data indicate the production of specific IgG in the blood serum of people in 2–3 weeks after SARSCov-2 infection and reaching its maximum level on the 20–21st day. The seroconversion rate was 94.9 %. It was shown that the geometric mean titer of antibodies in asymptomatic and mild forms of coronavirus infection did not differ statistically and amounted to 1:512 and 1:632, respectively. Higher titers of antibodies (1:1600) were detected in the moderate form.Conclusion. The research results can serve as a basis for studying the dynamics of changes in the indicators of the humoral immune response in patients with COVID-19 and for clarifying the duration of their post- infectious immunity in order to predict the development of the epidemic situation and to ensure the planning of specific prevention.
format	article
author	S. V. Balakhonov V. I. Dubrovina M. V. Chesnokova V. V. Voitkova A. B. Pyatidesyatnikova D. D. Bryukhova N. O. Kiseleva K. M. Korytov T. G. Kuznetsova Zh. V. Markevich
author_facet	S. V. Balakhonov V. I. Dubrovina M. V. Chesnokova V. V. Voitkova A. B. Pyatidesyatnikova D. D. Bryukhova N. O. Kiseleva K. M. Korytov T. G. Kuznetsova Zh. V. Markevich
author_sort	S. V. Balakhonov
title	Studying Humoral Immune Response at Mild and Asymptomatic COVID-19 Forms
title_short	Studying Humoral Immune Response at Mild and Asymptomatic COVID-19 Forms
title_full	Studying Humoral Immune Response at Mild and Asymptomatic COVID-19 Forms
title_fullStr	Studying Humoral Immune Response at Mild and Asymptomatic COVID-19 Forms
title_full_unstemmed	Studying Humoral Immune Response at Mild and Asymptomatic COVID-19 Forms
title_sort	studying humoral immune response at mild and asymptomatic covid-19 forms
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2020
url	https://doaj.org/article/67b0c32d84344077850233a511edb718
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Studying Humoral Immune Response at Mild and Asymptomatic COVID-19 Forms

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