Two New SGI1-LK Variants Found in <named-content content-type="genus-species">Proteus mirabilis</named-content> and Evolution of the SGI1-HKL Group of <italic toggle="yes">Salmonella</italic> Genomic Islands

ABSTRACT Integrative mobilizable elements belonging to the SGI1-H, -K, and -L Salmonella genomic island 1 (SGI1) variant groups are distinguished by the presence of an alteration in the backbone (IS1359 replaces 2.8 kb of the backbone extending from within traN [S005] to within S009). Members of thi...

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Autores principales:	Claire de Curraize, Eliane Siebor, Véronique Varin, Catherine Neuwirth, Ruth M. Hall
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Salmonella genomic island 1 Proteus mirabilis IS26 evolution SGI1 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/67b2f33393264e28b4dfd25b637e2a9d
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spelling	oai:doaj.org-article:67b2f33393264e28b4dfd25b637e2a9d2021-11-15T15:29:16ZTwo New SGI1-LK Variants Found in <named-content content-type="genus-species">Proteus mirabilis</named-content> and Evolution of the SGI1-HKL Group of <italic toggle="yes">Salmonella</italic> Genomic Islands10.1128/mSphere.00875-192379-5042https://doaj.org/article/67b2f33393264e28b4dfd25b637e2a9d2020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00875-19https://doaj.org/toc/2379-5042ABSTRACT Integrative mobilizable elements belonging to the SGI1-H, -K, and -L Salmonella genomic island 1 (SGI1) variant groups are distinguished by the presence of an alteration in the backbone (IS1359 replaces 2.8 kb of the backbone extending from within traN [S005] to within S009). Members of this SGI1-HKL group have been found in Salmonella enterica serovars and in Proteus mirabilis. Two novel variants from this group, designated SGI1-LK1 and SGI1-LK2, were found in the draft genomes of antibiotic-resistant P. mirabilis isolates from two French hospitals. Both variants can be derived from SGI1-PmGUE, a configuration found previously in another P. mirabilis isolate from France. SGI1-LK1 could arise via an IS26-mediated inversion in the complex class 1 integron that duplicated the IS26 element and the target site in IS6100. SGI1-LK1 also has a larger 8.59-kb backbone deletion extending from traN to within S013 and removing traG and traH. However, SGI1-LK1 was mobilized by an IncC plasmid. SGI1-LK2 can be derived from a hypothetical progenitor, SGI1-LK0, that is related to SGI1-PmGUE but lacks the aphA1 gene and one copy of IS26. The integron of SGI1-LK2 could arise via deletion of DNA adjacent to an IS26 and a deletion occurring via homologous recombination between duplicated copies of part of the integron 3′-conserved segment. SGI1-K can also be derived from SGI1-LK0. This would involve an IS26-mediated deletion and an inversion via homologous recombination of a segment between inversely oriented IS26s. Similar events can explain the configuration of the integrons in other SGI1-LK variants. IMPORTANCE Members of the SGI1-HKL subgroup of SGI1-type integrative mobilizable elements have a characteristic alteration in their backbone. They are widely distributed among multiply antibiotic-resistant Salmonella enterica serovars and Proteus mirabilis isolates. The SGI1-K type, found in the globally disseminated multiply antibiotic-resistant Salmonella enterica serovar Kentucky clone ST198 (sequence type 198), and various configurations in the original SGI1-LK group, found in other multiresistant S. enterica serovars and Proteus mirabilis isolates, have complex and highly plastic resistance regions due to the presence of IS26. However, how these complex forms arose and the relationships between them had not been analyzed. Here, a hypothetical progenitor, SGI1-LK0, that can be formed from the simpler SGI1-H is proposed, and the pathways to the formation of new variants, SGI1-LK1 and SGI1-LK2, found in P. mirabilis and other reported configurations via homologous recombination and IS26-mediated events are proposed. This led to a better understanding of the evolution of the SGI1-HKL group.Claire de CurraizeEliane SieborVéronique VarinCatherine NeuwirthRuth M. HallAmerican Society for MicrobiologyarticleSalmonella genomic island 1Proteus mirabilisIS26evolutionSGI1MicrobiologyQR1-502ENmSphere, Vol 5, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Salmonella genomic island 1 Proteus mirabilis IS26 evolution SGI1 Microbiology QR1-502
spellingShingle	Salmonella genomic island 1 Proteus mirabilis IS26 evolution SGI1 Microbiology QR1-502 Claire de Curraize Eliane Siebor Véronique Varin Catherine Neuwirth Ruth M. Hall Two New SGI1-LK Variants Found in <named-content content-type="genus-species">Proteus mirabilis</named-content> and Evolution of the SGI1-HKL Group of <italic toggle="yes">Salmonella</italic> Genomic Islands
description	ABSTRACT Integrative mobilizable elements belonging to the SGI1-H, -K, and -L Salmonella genomic island 1 (SGI1) variant groups are distinguished by the presence of an alteration in the backbone (IS1359 replaces 2.8 kb of the backbone extending from within traN [S005] to within S009). Members of this SGI1-HKL group have been found in Salmonella enterica serovars and in Proteus mirabilis. Two novel variants from this group, designated SGI1-LK1 and SGI1-LK2, were found in the draft genomes of antibiotic-resistant P. mirabilis isolates from two French hospitals. Both variants can be derived from SGI1-PmGUE, a configuration found previously in another P. mirabilis isolate from France. SGI1-LK1 could arise via an IS26-mediated inversion in the complex class 1 integron that duplicated the IS26 element and the target site in IS6100. SGI1-LK1 also has a larger 8.59-kb backbone deletion extending from traN to within S013 and removing traG and traH. However, SGI1-LK1 was mobilized by an IncC plasmid. SGI1-LK2 can be derived from a hypothetical progenitor, SGI1-LK0, that is related to SGI1-PmGUE but lacks the aphA1 gene and one copy of IS26. The integron of SGI1-LK2 could arise via deletion of DNA adjacent to an IS26 and a deletion occurring via homologous recombination between duplicated copies of part of the integron 3′-conserved segment. SGI1-K can also be derived from SGI1-LK0. This would involve an IS26-mediated deletion and an inversion via homologous recombination of a segment between inversely oriented IS26s. Similar events can explain the configuration of the integrons in other SGI1-LK variants. IMPORTANCE Members of the SGI1-HKL subgroup of SGI1-type integrative mobilizable elements have a characteristic alteration in their backbone. They are widely distributed among multiply antibiotic-resistant Salmonella enterica serovars and Proteus mirabilis isolates. The SGI1-K type, found in the globally disseminated multiply antibiotic-resistant Salmonella enterica serovar Kentucky clone ST198 (sequence type 198), and various configurations in the original SGI1-LK group, found in other multiresistant S. enterica serovars and Proteus mirabilis isolates, have complex and highly plastic resistance regions due to the presence of IS26. However, how these complex forms arose and the relationships between them had not been analyzed. Here, a hypothetical progenitor, SGI1-LK0, that can be formed from the simpler SGI1-H is proposed, and the pathways to the formation of new variants, SGI1-LK1 and SGI1-LK2, found in P. mirabilis and other reported configurations via homologous recombination and IS26-mediated events are proposed. This led to a better understanding of the evolution of the SGI1-HKL group.
format	article
author	Claire de Curraize Eliane Siebor Véronique Varin Catherine Neuwirth Ruth M. Hall
author_facet	Claire de Curraize Eliane Siebor Véronique Varin Catherine Neuwirth Ruth M. Hall
author_sort	Claire de Curraize
title	Two New SGI1-LK Variants Found in <named-content content-type="genus-species">Proteus mirabilis</named-content> and Evolution of the SGI1-HKL Group of <italic toggle="yes">Salmonella</italic> Genomic Islands
title_short	Two New SGI1-LK Variants Found in <named-content content-type="genus-species">Proteus mirabilis</named-content> and Evolution of the SGI1-HKL Group of <italic toggle="yes">Salmonella</italic> Genomic Islands
title_full	Two New SGI1-LK Variants Found in <named-content content-type="genus-species">Proteus mirabilis</named-content> and Evolution of the SGI1-HKL Group of <italic toggle="yes">Salmonella</italic> Genomic Islands
title_fullStr	Two New SGI1-LK Variants Found in <named-content content-type="genus-species">Proteus mirabilis</named-content> and Evolution of the SGI1-HKL Group of <italic toggle="yes">Salmonella</italic> Genomic Islands
title_full_unstemmed	Two New SGI1-LK Variants Found in <named-content content-type="genus-species">Proteus mirabilis</named-content> and Evolution of the SGI1-HKL Group of <italic toggle="yes">Salmonella</italic> Genomic Islands
title_sort	two new sgi1-lk variants found in <named-content content-type="genus-species">proteus mirabilis</named-content> and evolution of the sgi1-hkl group of <italic toggle="yes">salmonella</italic> genomic islands
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/67b2f33393264e28b4dfd25b637e2a9d
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Two New SGI1-LK Variants Found in <named-content content-type="genus-species">Proteus mirabilis</named-content> and Evolution of the SGI1-HKL Group of <italic toggle="yes">Salmonella</italic> Genomic Islands

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