ABA signalling and metabolism are not essential for dark-induced stomatal closure but affect response speed

Abstract Stomata are microscopic pores that open and close, acting to balance CO2 uptake with water loss. Stomata close in response to various signals including the drought hormone abscisic acid (ABA), microbe-associated-molecular-patterns, high CO2 levels, and darkness. The signalling pathways unde...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ashley J. Pridgeon, Alistair M. Hetherington
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/67b75a3fe7694ceaa3af6b7ffcd63f97
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:67b75a3fe7694ceaa3af6b7ffcd63f97
record_format	dspace
spelling	oai:doaj.org-article:67b75a3fe7694ceaa3af6b7ffcd63f972021-12-02T13:20:12ZABA signalling and metabolism are not essential for dark-induced stomatal closure but affect response speed10.1038/s41598-021-84911-52045-2322https://doaj.org/article/67b75a3fe7694ceaa3af6b7ffcd63f972021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84911-5https://doaj.org/toc/2045-2322Abstract Stomata are microscopic pores that open and close, acting to balance CO2 uptake with water loss. Stomata close in response to various signals including the drought hormone abscisic acid (ABA), microbe-associated-molecular-patterns, high CO2 levels, and darkness. The signalling pathways underlying ABA-induced stomatal closure are well known, however, the mechanism for dark-induced stomatal closure is less clear. ABA signalling has been suggested to play a role in dark-induced stomatal closure, but it is unclear how this occurs. Here we investigate the role of ABA in promoting dark-induced stomatal closure. Tracking stomatal movements on the surface of leaf discs we find, although steady state stomatal apertures are affected by mutations in ABA signalling and metabolism genes, all mutants investigated close in response to darkness. However, we observed a delayed response to darkness for certain ABA signalling and metabolism mutants. Investigating this further in the quadruple ABA receptor mutant (pyr1pyl1pyl2pyl4), compared with wild-type, we found reduced stomatal conductance kinetics. Although our results suggest a non-essential role for ABA in dark-induced stomatal closure, we show that ABA modulates the speed of the dark-induced closure response. These results highlight the role of ABA signalling and metabolic pathways as potential targets for enhancing stomatal movement kinetics.Ashley J. PridgeonAlistair M. HetheringtonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ashley J. Pridgeon Alistair M. Hetherington ABA signalling and metabolism are not essential for dark-induced stomatal closure but affect response speed
description	Abstract Stomata are microscopic pores that open and close, acting to balance CO2 uptake with water loss. Stomata close in response to various signals including the drought hormone abscisic acid (ABA), microbe-associated-molecular-patterns, high CO2 levels, and darkness. The signalling pathways underlying ABA-induced stomatal closure are well known, however, the mechanism for dark-induced stomatal closure is less clear. ABA signalling has been suggested to play a role in dark-induced stomatal closure, but it is unclear how this occurs. Here we investigate the role of ABA in promoting dark-induced stomatal closure. Tracking stomatal movements on the surface of leaf discs we find, although steady state stomatal apertures are affected by mutations in ABA signalling and metabolism genes, all mutants investigated close in response to darkness. However, we observed a delayed response to darkness for certain ABA signalling and metabolism mutants. Investigating this further in the quadruple ABA receptor mutant (pyr1pyl1pyl2pyl4), compared with wild-type, we found reduced stomatal conductance kinetics. Although our results suggest a non-essential role for ABA in dark-induced stomatal closure, we show that ABA modulates the speed of the dark-induced closure response. These results highlight the role of ABA signalling and metabolic pathways as potential targets for enhancing stomatal movement kinetics.
format	article
author	Ashley J. Pridgeon Alistair M. Hetherington
author_facet	Ashley J. Pridgeon Alistair M. Hetherington
author_sort	Ashley J. Pridgeon
title	ABA signalling and metabolism are not essential for dark-induced stomatal closure but affect response speed
title_short	ABA signalling and metabolism are not essential for dark-induced stomatal closure but affect response speed
title_full	ABA signalling and metabolism are not essential for dark-induced stomatal closure but affect response speed
title_fullStr	ABA signalling and metabolism are not essential for dark-induced stomatal closure but affect response speed
title_full_unstemmed	ABA signalling and metabolism are not essential for dark-induced stomatal closure but affect response speed
title_sort	aba signalling and metabolism are not essential for dark-induced stomatal closure but affect response speed
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/67b75a3fe7694ceaa3af6b7ffcd63f97
work_keys_str_mv	AT ashleyjpridgeon abasignallingandmetabolismarenotessentialfordarkinducedstomatalclosurebutaffectresponsespeed AT alistairmhetherington abasignallingandmetabolismarenotessentialfordarkinducedstomatalclosurebutaffectresponsespeed
_version_	1718393216131137536

ABA signalling and metabolism are not essential for dark-induced stomatal closure but affect response speed

Ejemplares similares