Gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels

Abstract During radiotherapy, microenvironments neighboring the tumor are also exposed to gamma irradiation; this results in unexpected side effects. Blood vessels can serve as microenvironments for tumors and they play an important role in providing nutrients to tumors. This is mostly related to tu...

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Autores principales:	Kyuhwan Na, Youngkyu Cho, Dong-Hee Choi, Myung-Jin Park, Ji-hun Yang, Seok Chung
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/67bc612d9cdb4e38a4fb932401831a5f
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spelling	oai:doaj.org-article:67bc612d9cdb4e38a4fb932401831a5f2021-12-02T15:16:10ZGamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels10.1038/s41598-021-97692-82045-2322https://doaj.org/article/67bc612d9cdb4e38a4fb932401831a5f2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97692-8https://doaj.org/toc/2045-2322Abstract During radiotherapy, microenvironments neighboring the tumor are also exposed to gamma irradiation; this results in unexpected side effects. Blood vessels can serve as microenvironments for tumors and they play an important role in providing nutrients to tumors. This is mostly related to tumor progression, metastasis, and relapse after therapy. Many studies have been performed to obtain a better understanding of tumor vasculature after radiotherapy with in vitro models. However, compared to 3-D models, 2-D in vitro endothelial monolayers cannot physiologically reflect in vivo blood vessels. We previously remodeled the extracellular matrix (ECM) hydrogel that enhanced the tight barrier formation of 3-D blood vessels and the vascular endothelial growth factor (VEGF) gradient induced angiogenesis in a microfluidic device. In this study, the blood vessel model is further introduced to understand how gamma irradiation affects the endothelial monolayer. After the gamma irradiation exposure, we observed a collapsed endothelial barrier and a reduced angiogenic potential. Changes in the cell behaviors of the tip and stalk cells were also detected in the angiogenesis model after irradiation, which is difficult to observe in 2-D monolayer models. Therefore, the 3-D in vitro blood vessel model can be used to understand radiation-induced endothelial injuries.Kyuhwan NaYoungkyu ChoDong-Hee ChoiMyung-Jin ParkJi-hun YangSeok ChungNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kyuhwan Na Youngkyu Cho Dong-Hee Choi Myung-Jin Park Ji-hun Yang Seok Chung Gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels
description	Abstract During radiotherapy, microenvironments neighboring the tumor are also exposed to gamma irradiation; this results in unexpected side effects. Blood vessels can serve as microenvironments for tumors and they play an important role in providing nutrients to tumors. This is mostly related to tumor progression, metastasis, and relapse after therapy. Many studies have been performed to obtain a better understanding of tumor vasculature after radiotherapy with in vitro models. However, compared to 3-D models, 2-D in vitro endothelial monolayers cannot physiologically reflect in vivo blood vessels. We previously remodeled the extracellular matrix (ECM) hydrogel that enhanced the tight barrier formation of 3-D blood vessels and the vascular endothelial growth factor (VEGF) gradient induced angiogenesis in a microfluidic device. In this study, the blood vessel model is further introduced to understand how gamma irradiation affects the endothelial monolayer. After the gamma irradiation exposure, we observed a collapsed endothelial barrier and a reduced angiogenic potential. Changes in the cell behaviors of the tip and stalk cells were also detected in the angiogenesis model after irradiation, which is difficult to observe in 2-D monolayer models. Therefore, the 3-D in vitro blood vessel model can be used to understand radiation-induced endothelial injuries.
format	article
author	Kyuhwan Na Youngkyu Cho Dong-Hee Choi Myung-Jin Park Ji-hun Yang Seok Chung
author_facet	Kyuhwan Na Youngkyu Cho Dong-Hee Choi Myung-Jin Park Ji-hun Yang Seok Chung
author_sort	Kyuhwan Na
title	Gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels
title_short	Gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels
title_full	Gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels
title_fullStr	Gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels
title_full_unstemmed	Gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels
title_sort	gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-d in vitro blood vessels
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/67bc612d9cdb4e38a4fb932401831a5f
work_keys_str_mv	AT kyuhwanna gammairradiationexposureforcollapsedcelljunctionsandreducedangiogenesisof3dinvitrobloodvessels AT youngkyucho gammairradiationexposureforcollapsedcelljunctionsandreducedangiogenesisof3dinvitrobloodvessels AT dongheechoi gammairradiationexposureforcollapsedcelljunctionsandreducedangiogenesisof3dinvitrobloodvessels AT myungjinpark gammairradiationexposureforcollapsedcelljunctionsandreducedangiogenesisof3dinvitrobloodvessels AT jihunyang gammairradiationexposureforcollapsedcelljunctionsandreducedangiogenesisof3dinvitrobloodvessels AT seokchung gammairradiationexposureforcollapsedcelljunctionsandreducedangiogenesisof3dinvitrobloodvessels
_version_	1718387511011573760

Gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels

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