RETRACTED ARTICLE: New molecular tools in Neospora caninum for studying apicomplexan parasite proteins

Abstract The development of molecular genetics has greatly enhanced the study of the biology and pathology associated with parasites of the phylum Apicomplexa. We have established a system specifically designed for Neospora caninum, and used this system as a heterologous platform for the expression...

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Autores principales:	Caroline M. Mota, Allan L. Chen, Kevin Wang, Santhosh Nadipuram, Ajay A. Vashisht, James A. Wohlschlegel, Tiago W. P. Mineo, Peter J. Bradley
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/67bd85191a8e480088804d6e05943eac
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spelling	oai:doaj.org-article:67bd85191a8e480088804d6e05943eac2021-12-02T16:06:26ZRETRACTED ARTICLE: New molecular tools in Neospora caninum for studying apicomplexan parasite proteins10.1038/s41598-017-03978-12045-2322https://doaj.org/article/67bd85191a8e480088804d6e05943eac2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03978-1https://doaj.org/toc/2045-2322Abstract The development of molecular genetics has greatly enhanced the study of the biology and pathology associated with parasites of the phylum Apicomplexa. We have established a system specifically designed for Neospora caninum, and used this system as a heterologous platform for the expression of foreign genes. Plasmid constructs containing fluorescent proteins or targeted genes of Toxoplasma gondii, driven by N. caninum promoters, have yielded robust expression and correct trafficking of target gene products as assessed by immunofluorescence assays and Western blot analyses. Using this approach, we here demonstrated that N. caninum expressing T. gondii’s GRA15 and ROP16 kinase are biologically active and induced immunological phenotypes consistent with T. gondii strains. N. caninum expressing TgGRA15 differentially disturbed the NF-κB pathway, inducing an increased IL-12 production. On the other hand, N. caninum expressing TgROP16 induced host STAT3 phosphorylation and consequent reduction of IL-12 synthesis. These results indicate that heterologous gene expression in N. caninum is a useful tool for the study of specific gene functions and may allow the identification of antigenic targets responsible for the phenotypic differences observed between these two closely related apicomplexan parasites. Additionally, these observations may prove to be useful for the development of vaccine protocols to control toxoplasmosis and/or neosporosis.Caroline M. MotaAllan L. ChenKevin WangSanthosh NadipuramAjay A. VashishtJames A. WohlschlegelTiago W. P. MineoPeter J. BradleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Caroline M. Mota Allan L. Chen Kevin Wang Santhosh Nadipuram Ajay A. Vashisht James A. Wohlschlegel Tiago W. P. Mineo Peter J. Bradley RETRACTED ARTICLE: New molecular tools in Neospora caninum for studying apicomplexan parasite proteins
description	Abstract The development of molecular genetics has greatly enhanced the study of the biology and pathology associated with parasites of the phylum Apicomplexa. We have established a system specifically designed for Neospora caninum, and used this system as a heterologous platform for the expression of foreign genes. Plasmid constructs containing fluorescent proteins or targeted genes of Toxoplasma gondii, driven by N. caninum promoters, have yielded robust expression and correct trafficking of target gene products as assessed by immunofluorescence assays and Western blot analyses. Using this approach, we here demonstrated that N. caninum expressing T. gondii’s GRA15 and ROP16 kinase are biologically active and induced immunological phenotypes consistent with T. gondii strains. N. caninum expressing TgGRA15 differentially disturbed the NF-κB pathway, inducing an increased IL-12 production. On the other hand, N. caninum expressing TgROP16 induced host STAT3 phosphorylation and consequent reduction of IL-12 synthesis. These results indicate that heterologous gene expression in N. caninum is a useful tool for the study of specific gene functions and may allow the identification of antigenic targets responsible for the phenotypic differences observed between these two closely related apicomplexan parasites. Additionally, these observations may prove to be useful for the development of vaccine protocols to control toxoplasmosis and/or neosporosis.
format	article
author	Caroline M. Mota Allan L. Chen Kevin Wang Santhosh Nadipuram Ajay A. Vashisht James A. Wohlschlegel Tiago W. P. Mineo Peter J. Bradley
author_facet	Caroline M. Mota Allan L. Chen Kevin Wang Santhosh Nadipuram Ajay A. Vashisht James A. Wohlschlegel Tiago W. P. Mineo Peter J. Bradley
author_sort	Caroline M. Mota
title	RETRACTED ARTICLE: New molecular tools in Neospora caninum for studying apicomplexan parasite proteins
title_short	RETRACTED ARTICLE: New molecular tools in Neospora caninum for studying apicomplexan parasite proteins
title_full	RETRACTED ARTICLE: New molecular tools in Neospora caninum for studying apicomplexan parasite proteins
title_fullStr	RETRACTED ARTICLE: New molecular tools in Neospora caninum for studying apicomplexan parasite proteins
title_full_unstemmed	RETRACTED ARTICLE: New molecular tools in Neospora caninum for studying apicomplexan parasite proteins
title_sort	retracted article: new molecular tools in neospora caninum for studying apicomplexan parasite proteins
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/67bd85191a8e480088804d6e05943eac
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RETRACTED ARTICLE: New molecular tools in Neospora caninum for studying apicomplexan parasite proteins

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