miR-362-3p suppresses ovarian cancer by inhibiting LRP8

Background: Ovarian cancer is one of the most common female cancers, with a high incidence worldwide. Aberrant expression of low‐density lipoprotein (LDL) receptor‐related protein 8 (LRP8) and microRNA (miR)-362-3p is involved in the pathogenesis of different cancers. Methods: We aimed to elucidate...

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Autores principales:	Chun Li, Yi Yang, Huimin Wang, Yu Song, Huan Huang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	miR-362-3p L Ovarian cancer Growth Cancer treatment Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/67c2494b59f3474daa2912d4ad9714d4
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spelling	oai:doaj.org-article:67c2494b59f3474daa2912d4ad9714d42021-11-28T04:29:52ZmiR-362-3p suppresses ovarian cancer by inhibiting LRP81936-523310.1016/j.tranon.2021.101284https://doaj.org/article/67c2494b59f3474daa2912d4ad9714d42022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1936523321002758https://doaj.org/toc/1936-5233Background: Ovarian cancer is one of the most common female cancers, with a high incidence worldwide. Aberrant expression of low‐density lipoprotein (LDL) receptor‐related protein 8 (LRP8) and microRNA (miR)-362-3p is involved in the pathogenesis of different cancers. Methods: We aimed to elucidate the underlying mechanism of the miR-362-3p-LRP8 axis in ovarian cancer. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to examine miR-362-3p and LRP8 expression in ovarian cancer tissues and cells. The luciferase assay was used to determine the relationship between miR-362-3p and LRP8. The function of overexpression of miR-362-3p and LRP8 was determined by assessing the cell viability using the cell counting kit 8 (CCK-8) assay, proliferation using 5′‑bromo-2′-deoxyuridine (BrdU) assay, migration using wound healing assay, invasion using transwell assay, and the protein expression levels of matrix metalloproteinase (MMP)-2, MMP9, and integrin α5 or β1 using western blotting assays in ovarian cancer cells. Results: miR-362-3p expression levels were decreased in ovarian cancer tissues and cells and negatively correlated with LRP8 levels. Overexpression of miR-362-3p dramatically repressed cell growth. Furthermore, overexpression of LRP8 significantly facilitated the proliferation, migration, and invasion of ovarian cancer cells, which counteracted the inhibitory effect of miR-362-3p on ovarian cancer cell growth. Conclusions: We reported that miR-362-3p hampered cell growth by repressing LRP8 expression in ovarian cancer cells. Our results provide new insights into ovarian cancer, involving both miR-362-3p and LRP8, which can be used as potential biomarkers for the treatment of ovarian cancer.Chun LiYi YangHuimin WangYu SongHuan HuangElsevierarticlemiR-362-3pLOvarian cancerGrowthCancer treatmentNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENTranslational Oncology, Vol 15, Iss 1, Pp 101284- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	miR-362-3p L Ovarian cancer Growth Cancer treatment Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	miR-362-3p L Ovarian cancer Growth Cancer treatment Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Chun Li Yi Yang Huimin Wang Yu Song Huan Huang miR-362-3p suppresses ovarian cancer by inhibiting LRP8
description	Background: Ovarian cancer is one of the most common female cancers, with a high incidence worldwide. Aberrant expression of low‐density lipoprotein (LDL) receptor‐related protein 8 (LRP8) and microRNA (miR)-362-3p is involved in the pathogenesis of different cancers. Methods: We aimed to elucidate the underlying mechanism of the miR-362-3p-LRP8 axis in ovarian cancer. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to examine miR-362-3p and LRP8 expression in ovarian cancer tissues and cells. The luciferase assay was used to determine the relationship between miR-362-3p and LRP8. The function of overexpression of miR-362-3p and LRP8 was determined by assessing the cell viability using the cell counting kit 8 (CCK-8) assay, proliferation using 5′‑bromo-2′-deoxyuridine (BrdU) assay, migration using wound healing assay, invasion using transwell assay, and the protein expression levels of matrix metalloproteinase (MMP)-2, MMP9, and integrin α5 or β1 using western blotting assays in ovarian cancer cells. Results: miR-362-3p expression levels were decreased in ovarian cancer tissues and cells and negatively correlated with LRP8 levels. Overexpression of miR-362-3p dramatically repressed cell growth. Furthermore, overexpression of LRP8 significantly facilitated the proliferation, migration, and invasion of ovarian cancer cells, which counteracted the inhibitory effect of miR-362-3p on ovarian cancer cell growth. Conclusions: We reported that miR-362-3p hampered cell growth by repressing LRP8 expression in ovarian cancer cells. Our results provide new insights into ovarian cancer, involving both miR-362-3p and LRP8, which can be used as potential biomarkers for the treatment of ovarian cancer.
format	article
author	Chun Li Yi Yang Huimin Wang Yu Song Huan Huang
author_facet	Chun Li Yi Yang Huimin Wang Yu Song Huan Huang
author_sort	Chun Li
title	miR-362-3p suppresses ovarian cancer by inhibiting LRP8
title_short	miR-362-3p suppresses ovarian cancer by inhibiting LRP8
title_full	miR-362-3p suppresses ovarian cancer by inhibiting LRP8
title_fullStr	miR-362-3p suppresses ovarian cancer by inhibiting LRP8
title_full_unstemmed	miR-362-3p suppresses ovarian cancer by inhibiting LRP8
title_sort	mir-362-3p suppresses ovarian cancer by inhibiting lrp8
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/67c2494b59f3474daa2912d4ad9714d4
work_keys_str_mv	AT chunli mir3623psuppressesovariancancerbyinhibitinglrp8 AT yiyang mir3623psuppressesovariancancerbyinhibitinglrp8 AT huiminwang mir3623psuppressesovariancancerbyinhibitinglrp8 AT yusong mir3623psuppressesovariancancerbyinhibitinglrp8 AT huanhuang mir3623psuppressesovariancancerbyinhibitinglrp8
_version_	1718408382028709888

miR-362-3p suppresses ovarian cancer by inhibiting LRP8

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