The anti-diabetic effects of NAG-1/GDF15 on HFD/STZ-induced mice
Abstract Nonsteroidal anti-inflammatory drug-activated gene-1 (NAG-1) plays a role in various diseases. Here, the anti-diabetic effects of NAG-1 were evaluated using a high-fat diet/streptozotocin-induced diabetic mouse model. NAG-1-overexpressing transgenic (NAG-1 Tg) mice exhibited lower body weig...
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Nature Portfolio
2021
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oai:doaj.org-article:67c795b40bd9439b92eb5d14ebf332072021-12-02T17:55:03ZThe anti-diabetic effects of NAG-1/GDF15 on HFD/STZ-induced mice10.1038/s41598-021-94581-y2045-2322https://doaj.org/article/67c795b40bd9439b92eb5d14ebf332072021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94581-yhttps://doaj.org/toc/2045-2322Abstract Nonsteroidal anti-inflammatory drug-activated gene-1 (NAG-1) plays a role in various diseases. Here, the anti-diabetic effects of NAG-1 were evaluated using a high-fat diet/streptozotocin-induced diabetic mouse model. NAG-1-overexpressing transgenic (NAG-1 Tg) mice exhibited lower body weight, fasting blood glucose levels, and serum insulin levels than wild-type (WT) mice. The homeostatic model assessment of insulin resistance scores of NAG-1 Tg mice were lower than those of WT mice. Hematoxylin and eosin staining revealed a smaller lipid droplet size in the adipose tissues, lower lipid accumulation in the hepatocytes, and larger beta cell area in the pancreas of NAG-1 Tg mice than in those of WT mice. Immunohistochemical analysis revealed downregulated expression of cleaved caspase-3, an apoptosis marker, in the beta cells of NAG-1 Tg mice. Adiponectin and leptin mRNA levels were upregulated and downregulated in NAG-1 Tg mice, respectively. Additionally, the expression of IRS1/PI3K/AKT signaling pathway components, especially Foxo1, which regulates gluconeogenesis in the muscle and white adipose tissue, was downregulated in NAG-1 Tg mice. Furthermore, NAG-1 overexpression promoted the expression of As160 in both muscles and adipocytes, and the mRNA levels of the NLRP3 pathway members were downregulated in NAG-1 Tg mice. Our findings suggest that NAG-1 expression alleviates diabetes in mice.Pattawika LertpatipanpongJaehak LeeIlju KimThomas ElingSeung Yeon OhJe Kyung SeongSeung Joon BaekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
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Medicine R Science Q Pattawika Lertpatipanpong Jaehak Lee Ilju Kim Thomas Eling Seung Yeon Oh Je Kyung Seong Seung Joon Baek The anti-diabetic effects of NAG-1/GDF15 on HFD/STZ-induced mice |
| description |
Abstract Nonsteroidal anti-inflammatory drug-activated gene-1 (NAG-1) plays a role in various diseases. Here, the anti-diabetic effects of NAG-1 were evaluated using a high-fat diet/streptozotocin-induced diabetic mouse model. NAG-1-overexpressing transgenic (NAG-1 Tg) mice exhibited lower body weight, fasting blood glucose levels, and serum insulin levels than wild-type (WT) mice. The homeostatic model assessment of insulin resistance scores of NAG-1 Tg mice were lower than those of WT mice. Hematoxylin and eosin staining revealed a smaller lipid droplet size in the adipose tissues, lower lipid accumulation in the hepatocytes, and larger beta cell area in the pancreas of NAG-1 Tg mice than in those of WT mice. Immunohistochemical analysis revealed downregulated expression of cleaved caspase-3, an apoptosis marker, in the beta cells of NAG-1 Tg mice. Adiponectin and leptin mRNA levels were upregulated and downregulated in NAG-1 Tg mice, respectively. Additionally, the expression of IRS1/PI3K/AKT signaling pathway components, especially Foxo1, which regulates gluconeogenesis in the muscle and white adipose tissue, was downregulated in NAG-1 Tg mice. Furthermore, NAG-1 overexpression promoted the expression of As160 in both muscles and adipocytes, and the mRNA levels of the NLRP3 pathway members were downregulated in NAG-1 Tg mice. Our findings suggest that NAG-1 expression alleviates diabetes in mice. |
| format |
article |
| author |
Pattawika Lertpatipanpong Jaehak Lee Ilju Kim Thomas Eling Seung Yeon Oh Je Kyung Seong Seung Joon Baek |
| author_facet |
Pattawika Lertpatipanpong Jaehak Lee Ilju Kim Thomas Eling Seung Yeon Oh Je Kyung Seong Seung Joon Baek |
| author_sort |
Pattawika Lertpatipanpong |
| title |
The anti-diabetic effects of NAG-1/GDF15 on HFD/STZ-induced mice |
| title_short |
The anti-diabetic effects of NAG-1/GDF15 on HFD/STZ-induced mice |
| title_full |
The anti-diabetic effects of NAG-1/GDF15 on HFD/STZ-induced mice |
| title_fullStr |
The anti-diabetic effects of NAG-1/GDF15 on HFD/STZ-induced mice |
| title_full_unstemmed |
The anti-diabetic effects of NAG-1/GDF15 on HFD/STZ-induced mice |
| title_sort |
anti-diabetic effects of nag-1/gdf15 on hfd/stz-induced mice |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/67c795b40bd9439b92eb5d14ebf33207 |
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