Rapid and sensitive detection of rotavirus molecular signatures using surface enhanced Raman spectroscopy.

Human enteric virus infections range from gastroenteritis to life threatening diseases such as myocarditis and aseptic meningitis. Rotavirus is one of the most common enteric agents and mortality associated with infection can be very significant in developing countries. Most enteric viruses produce...

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Autores principales:	Jeremy D Driskell, Yu Zhu, Carl D Kirkwood, Yiping Zhao, Richard A Dluhy, Ralph A Tripp
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/67cc2daa17574f9a8b97277c252bd712
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spelling	oai:doaj.org-article:67cc2daa17574f9a8b97277c252bd7122021-11-25T06:24:28ZRapid and sensitive detection of rotavirus molecular signatures using surface enhanced Raman spectroscopy.1932-620310.1371/journal.pone.0010222https://doaj.org/article/67cc2daa17574f9a8b97277c252bd7122010-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20419101/?tool=EBIhttps://doaj.org/toc/1932-6203Human enteric virus infections range from gastroenteritis to life threatening diseases such as myocarditis and aseptic meningitis. Rotavirus is one of the most common enteric agents and mortality associated with infection can be very significant in developing countries. Most enteric viruses produce diseases that are not distinct from other pathogens, and current diagnostics is limited in breadth and sensitivity required to advance virus detection schemes for disease intervention strategies. A spectroscopic assay based on surface enhanced Raman scattering (SERS) has been developed for rapid and sensitive detection of rotavirus. The SERS method relies on the fabrication of silver nanorod array substrates that are extremely SERS-active allowing for direct structural characterization of viruses. SERS spectra for eight rotavirus strains were analyzed to qualitatively identify rotaviruses and to classify each according to G and P genotype and strain with >96% accuracy, and a quantitative model based on partial least squares regression analysis was evaluated. This novel SERS-based virus detection method shows that SERS can be used to identify spectral fingerprints of human rotaviruses, and suggests that this detection method can be used for pathogen detection central to human health care.Jeremy D DriskellYu ZhuCarl D KirkwoodYiping ZhaoRichard A DluhyRalph A TrippPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 4, p e10222 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jeremy D Driskell Yu Zhu Carl D Kirkwood Yiping Zhao Richard A Dluhy Ralph A Tripp Rapid and sensitive detection of rotavirus molecular signatures using surface enhanced Raman spectroscopy.
description	Human enteric virus infections range from gastroenteritis to life threatening diseases such as myocarditis and aseptic meningitis. Rotavirus is one of the most common enteric agents and mortality associated with infection can be very significant in developing countries. Most enteric viruses produce diseases that are not distinct from other pathogens, and current diagnostics is limited in breadth and sensitivity required to advance virus detection schemes for disease intervention strategies. A spectroscopic assay based on surface enhanced Raman scattering (SERS) has been developed for rapid and sensitive detection of rotavirus. The SERS method relies on the fabrication of silver nanorod array substrates that are extremely SERS-active allowing for direct structural characterization of viruses. SERS spectra for eight rotavirus strains were analyzed to qualitatively identify rotaviruses and to classify each according to G and P genotype and strain with >96% accuracy, and a quantitative model based on partial least squares regression analysis was evaluated. This novel SERS-based virus detection method shows that SERS can be used to identify spectral fingerprints of human rotaviruses, and suggests that this detection method can be used for pathogen detection central to human health care.
format	article
author	Jeremy D Driskell Yu Zhu Carl D Kirkwood Yiping Zhao Richard A Dluhy Ralph A Tripp
author_facet	Jeremy D Driskell Yu Zhu Carl D Kirkwood Yiping Zhao Richard A Dluhy Ralph A Tripp
author_sort	Jeremy D Driskell
title	Rapid and sensitive detection of rotavirus molecular signatures using surface enhanced Raman spectroscopy.
title_short	Rapid and sensitive detection of rotavirus molecular signatures using surface enhanced Raman spectroscopy.
title_full	Rapid and sensitive detection of rotavirus molecular signatures using surface enhanced Raman spectroscopy.
title_fullStr	Rapid and sensitive detection of rotavirus molecular signatures using surface enhanced Raman spectroscopy.
title_full_unstemmed	Rapid and sensitive detection of rotavirus molecular signatures using surface enhanced Raman spectroscopy.
title_sort	rapid and sensitive detection of rotavirus molecular signatures using surface enhanced raman spectroscopy.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/67cc2daa17574f9a8b97277c252bd712
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_version_	1718413748416282624

Rapid and sensitive detection of rotavirus molecular signatures using surface enhanced Raman spectroscopy.

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