Mapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomography.

To date there is a lack of tools to map the spatio-temporal dynamics of diverse cells in experimental heart models. Conventional histology is labor intensive with limited coverage, whereas many imaging techniques do not have sufficiently high enough spatial resolution to map cell distributions. We h...

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Autores principales:	Claudio Vinegoni, Paolo Fumene Feruglio, Daniel Razansky, Rostic Gorbatov, Vasilis Ntziachristos, Andrea Sbarbati, Matthias Nahrendorf, Ralph Weissleder
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/67d2cfcb14aa4ca8b000b1d7217dc0be
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id	oai:doaj.org-article:67d2cfcb14aa4ca8b000b1d7217dc0be
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spelling	oai:doaj.org-article:67d2cfcb14aa4ca8b000b1d7217dc0be2021-11-18T07:22:39ZMapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomography.1932-620310.1371/journal.pone.0034427https://doaj.org/article/67d2cfcb14aa4ca8b000b1d7217dc0be2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22509302/?tool=EBIhttps://doaj.org/toc/1932-6203To date there is a lack of tools to map the spatio-temporal dynamics of diverse cells in experimental heart models. Conventional histology is labor intensive with limited coverage, whereas many imaging techniques do not have sufficiently high enough spatial resolution to map cell distributions. We have designed and built a high resolution, dual channel Born-normalized near-infrared fluorescence optical projection tomography system to quantitatively and spatially resolve molecular agents distribution within whole murine heart. We validated the use of the system in a mouse model of monocytes/macrophages recruitment during myocardial infarction. While acquired, data were processed and reconstructed in real time. Tomographic analysis and visualization of the key inflammatory components were obtained via a mathematical formalism based on left ventricular modeling. We observed extensive monocyte recruitment within and around the infarcted areas and discovered that monocytes were also extensively recruited into non-ischemic myocardium, beyond that of injured tissue, such as the septum.Claudio VinegoniPaolo Fumene FeruglioDaniel RazanskyRostic GorbatovVasilis NtziachristosAndrea SbarbatiMatthias NahrendorfRalph WeisslederPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e34427 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claudio Vinegoni Paolo Fumene Feruglio Daniel Razansky Rostic Gorbatov Vasilis Ntziachristos Andrea Sbarbati Matthias Nahrendorf Ralph Weissleder Mapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomography.
description	To date there is a lack of tools to map the spatio-temporal dynamics of diverse cells in experimental heart models. Conventional histology is labor intensive with limited coverage, whereas many imaging techniques do not have sufficiently high enough spatial resolution to map cell distributions. We have designed and built a high resolution, dual channel Born-normalized near-infrared fluorescence optical projection tomography system to quantitatively and spatially resolve molecular agents distribution within whole murine heart. We validated the use of the system in a mouse model of monocytes/macrophages recruitment during myocardial infarction. While acquired, data were processed and reconstructed in real time. Tomographic analysis and visualization of the key inflammatory components were obtained via a mathematical formalism based on left ventricular modeling. We observed extensive monocyte recruitment within and around the infarcted areas and discovered that monocytes were also extensively recruited into non-ischemic myocardium, beyond that of injured tissue, such as the septum.
format	article
author	Claudio Vinegoni Paolo Fumene Feruglio Daniel Razansky Rostic Gorbatov Vasilis Ntziachristos Andrea Sbarbati Matthias Nahrendorf Ralph Weissleder
author_facet	Claudio Vinegoni Paolo Fumene Feruglio Daniel Razansky Rostic Gorbatov Vasilis Ntziachristos Andrea Sbarbati Matthias Nahrendorf Ralph Weissleder
author_sort	Claudio Vinegoni
title	Mapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomography.
title_short	Mapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomography.
title_full	Mapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomography.
title_fullStr	Mapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomography.
title_full_unstemmed	Mapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomography.
title_sort	mapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomography.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/67d2cfcb14aa4ca8b000b1d7217dc0be
work_keys_str_mv	AT claudiovinegoni mappingmolecularagentsdistributionsinwholemiceheartsusingbornnormalizedopticalprojectiontomography AT paolofumeneferuglio mappingmolecularagentsdistributionsinwholemiceheartsusingbornnormalizedopticalprojectiontomography AT danielrazansky mappingmolecularagentsdistributionsinwholemiceheartsusingbornnormalizedopticalprojectiontomography AT rosticgorbatov mappingmolecularagentsdistributionsinwholemiceheartsusingbornnormalizedopticalprojectiontomography AT vasilisntziachristos mappingmolecularagentsdistributionsinwholemiceheartsusingbornnormalizedopticalprojectiontomography AT andreasbarbati mappingmolecularagentsdistributionsinwholemiceheartsusingbornnormalizedopticalprojectiontomography AT matthiasnahrendorf mappingmolecularagentsdistributionsinwholemiceheartsusingbornnormalizedopticalprojectiontomography AT ralphweissleder mappingmolecularagentsdistributionsinwholemiceheartsusingbornnormalizedopticalprojectiontomography
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Mapping molecular agents distributions in whole mice hearts using born-normalized optical projection tomography.

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