Effect of S100A6 on activation of hepatic stellate cells in mice

Objective To determine the expression of S100A6 in liver tissues and investigate its role in the activation of hepatic stellate cells (HSCs). Methods Twelve C57BL/6 mice were randomly assigned to control group and liver fibrosis group. The mice in the latter group was administrated with carbon tetra...

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Autores principales:	HU Zhen, ZHONG Li, DENG Liang, LIU Chang
Formato:	article
Lenguaje:	ZH
Publicado:	Editorial Office of Journal of Third Military Medical University 2021
Materias:	s100a6 hepatic stellate cells liver fibrosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/67dd1abf1e3942ab953ae981462aff2e
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spelling	oai:doaj.org-article:67dd1abf1e3942ab953ae981462aff2e2021-11-12T05:07:29ZEffect of S100A6 on activation of hepatic stellate cells in mice10.16016/j.1000-5404.2021041081000-5404https://doaj.org/article/67dd1abf1e3942ab953ae981462aff2e2021-11-01T00:00:00Zhttp://aammt.tmmu.edu.cn/Upload/rhtml/202104108.htmhttps://doaj.org/toc/1000-5404Objective To determine the expression of S100A6 in liver tissues and investigate its role in the activation of hepatic stellate cells (HSCs). Methods Twelve C57BL/6 mice were randomly assigned to control group and liver fibrosis group. The mice in the latter group was administrated with carbon tetrachloride for 6 weeks to induce liver fibrosis. The liver tissue was harvested for Masson staining and S100A6 detection by immunohistochemistry. The expression of S100A6 at mRNA and protein levels was detected by RT-qPCR and Western blotting. After primary HSCs were isolated and cultured, their morphological changes in quiescence and activation were observed, and the mRNA and protein levels of S100A6 were detected after cultured for different time periods. Additionally, siRNA was utilized to interfere S100A6 expression in HSCs, and the mRNA and protein levels of S100A6, Collagen Ⅰ and α-SMA were tested by RT-qPCR and Western blotting. Results Masson staining displayed fibrosis in liver tissue in the mice of the liver fibrosis group, and S100A6 was also significantly expressed in the corresponding fibrous cord area of liver tissue in liver fibrosis group, and its mRNA and protein levels were obviously enhanced in the liver tissue when compared to the control group (P < 0.05). In vitro, HSCs auto-activated with the process of culture, and the mRNA and protein expression levels of S100A6 were gradually increased in a time depended manner in the HSCs (P < 0.05 or P < 0.001). Undoubtedly, the interference of S100A6 by siRNA efficiency dramatically inhibited the mRNA and protein expression of S100A6, Collagen Ⅰ, α-SMA (P < 0.05 or P < 0.001). Conclusion S100A6 is specifically expressed in hepatic fibrosis and activated HSCs, and is an important protein regulating the activation of HSCs. Thus, S100A6 might play important role in the pathogenesis of liver fibrosis.HU ZhenZHONG LiDENG LiangLIU ChangEditorial Office of Journal of Third Military Medical Universityarticles100a6hepatic stellate cellsliver fibrosisMedicine (General)R5-920ZHDi-san junyi daxue xuebao, Vol 43, Iss 21, Pp 2375-2380 (2021)
institution	DOAJ
collection	DOAJ
language	ZH
topic	s100a6 hepatic stellate cells liver fibrosis Medicine (General) R5-920
spellingShingle	s100a6 hepatic stellate cells liver fibrosis Medicine (General) R5-920 HU Zhen ZHONG Li DENG Liang LIU Chang Effect of S100A6 on activation of hepatic stellate cells in mice
description	Objective To determine the expression of S100A6 in liver tissues and investigate its role in the activation of hepatic stellate cells (HSCs). Methods Twelve C57BL/6 mice were randomly assigned to control group and liver fibrosis group. The mice in the latter group was administrated with carbon tetrachloride for 6 weeks to induce liver fibrosis. The liver tissue was harvested for Masson staining and S100A6 detection by immunohistochemistry. The expression of S100A6 at mRNA and protein levels was detected by RT-qPCR and Western blotting. After primary HSCs were isolated and cultured, their morphological changes in quiescence and activation were observed, and the mRNA and protein levels of S100A6 were detected after cultured for different time periods. Additionally, siRNA was utilized to interfere S100A6 expression in HSCs, and the mRNA and protein levels of S100A6, Collagen Ⅰ and α-SMA were tested by RT-qPCR and Western blotting. Results Masson staining displayed fibrosis in liver tissue in the mice of the liver fibrosis group, and S100A6 was also significantly expressed in the corresponding fibrous cord area of liver tissue in liver fibrosis group, and its mRNA and protein levels were obviously enhanced in the liver tissue when compared to the control group (P < 0.05). In vitro, HSCs auto-activated with the process of culture, and the mRNA and protein expression levels of S100A6 were gradually increased in a time depended manner in the HSCs (P < 0.05 or P < 0.001). Undoubtedly, the interference of S100A6 by siRNA efficiency dramatically inhibited the mRNA and protein expression of S100A6, Collagen Ⅰ, α-SMA (P < 0.05 or P < 0.001). Conclusion S100A6 is specifically expressed in hepatic fibrosis and activated HSCs, and is an important protein regulating the activation of HSCs. Thus, S100A6 might play important role in the pathogenesis of liver fibrosis.
format	article
author	HU Zhen ZHONG Li DENG Liang LIU Chang
author_facet	HU Zhen ZHONG Li DENG Liang LIU Chang
author_sort	HU Zhen
title	Effect of S100A6 on activation of hepatic stellate cells in mice
title_short	Effect of S100A6 on activation of hepatic stellate cells in mice
title_full	Effect of S100A6 on activation of hepatic stellate cells in mice
title_fullStr	Effect of S100A6 on activation of hepatic stellate cells in mice
title_full_unstemmed	Effect of S100A6 on activation of hepatic stellate cells in mice
title_sort	effect of s100a6 on activation of hepatic stellate cells in mice
publisher	Editorial Office of Journal of Third Military Medical University
publishDate	2021
url	https://doaj.org/article/67dd1abf1e3942ab953ae981462aff2e
work_keys_str_mv	AT huzhen effectofs100a6onactivationofhepaticstellatecellsinmice AT zhongli effectofs100a6onactivationofhepaticstellatecellsinmice AT dengliang effectofs100a6onactivationofhepaticstellatecellsinmice AT liuchang effectofs100a6onactivationofhepaticstellatecellsinmice
_version_	1718431174514180096

Effect of S100A6 on activation of hepatic stellate cells in mice

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