SRpHi ratiometric pH biosensors for super-resolution microscopy

Ratiometric fluorescent pH probes are useful tools to monitor acidification of vesicles during endocytosis, but the size of vesicles is below the diffraction limit. Here the authors develop a family of ratiometric pH sensors for use in STED super-resolution microscopy, and optimize their delivery to...

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Autores principales: Douglas S. Richardson, Carola Gregor, Franziska R. Winter, Nicolai T. Urban, Steffen J. Sahl, Katrin I. Willig, Stefan W. Hell
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/67dd8700237c48b588b132e8702e7c81
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Sumario:Ratiometric fluorescent pH probes are useful tools to monitor acidification of vesicles during endocytosis, but the size of vesicles is below the diffraction limit. Here the authors develop a family of ratiometric pH sensors for use in STED super-resolution microscopy, and optimize their delivery to endosomes.