SRpHi ratiometric pH biosensors for super-resolution microscopy

Ratiometric fluorescent pH probes are useful tools to monitor acidification of vesicles during endocytosis, but the size of vesicles is below the diffraction limit. Here the authors develop a family of ratiometric pH sensors for use in STED super-resolution microscopy, and optimize their delivery to...

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Autores principales:	Douglas S. Richardson, Carola Gregor, Franziska R. Winter, Nicolai T. Urban, Steffen J. Sahl, Katrin I. Willig, Stefan W. Hell
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/67dd8700237c48b588b132e8702e7c81
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spelling	oai:doaj.org-article:67dd8700237c48b588b132e8702e7c812021-12-02T17:06:12ZSRpHi ratiometric pH biosensors for super-resolution microscopy10.1038/s41467-017-00606-42041-1723https://doaj.org/article/67dd8700237c48b588b132e8702e7c812017-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00606-4https://doaj.org/toc/2041-1723Ratiometric fluorescent pH probes are useful tools to monitor acidification of vesicles during endocytosis, but the size of vesicles is below the diffraction limit. Here the authors develop a family of ratiometric pH sensors for use in STED super-resolution microscopy, and optimize their delivery to endosomes.Douglas S. RichardsonCarola GregorFranziska R. WinterNicolai T. UrbanSteffen J. SahlKatrin I. WilligStefan W. HellNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Douglas S. Richardson Carola Gregor Franziska R. Winter Nicolai T. Urban Steffen J. Sahl Katrin I. Willig Stefan W. Hell SRpHi ratiometric pH biosensors for super-resolution microscopy
description	Ratiometric fluorescent pH probes are useful tools to monitor acidification of vesicles during endocytosis, but the size of vesicles is below the diffraction limit. Here the authors develop a family of ratiometric pH sensors for use in STED super-resolution microscopy, and optimize their delivery to endosomes.
format	article
author	Douglas S. Richardson Carola Gregor Franziska R. Winter Nicolai T. Urban Steffen J. Sahl Katrin I. Willig Stefan W. Hell
author_facet	Douglas S. Richardson Carola Gregor Franziska R. Winter Nicolai T. Urban Steffen J. Sahl Katrin I. Willig Stefan W. Hell
author_sort	Douglas S. Richardson
title	SRpHi ratiometric pH biosensors for super-resolution microscopy
title_short	SRpHi ratiometric pH biosensors for super-resolution microscopy
title_full	SRpHi ratiometric pH biosensors for super-resolution microscopy
title_fullStr	SRpHi ratiometric pH biosensors for super-resolution microscopy
title_full_unstemmed	SRpHi ratiometric pH biosensors for super-resolution microscopy
title_sort	srphi ratiometric ph biosensors for super-resolution microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/67dd8700237c48b588b132e8702e7c81
work_keys_str_mv	AT douglassrichardson srphiratiometricphbiosensorsforsuperresolutionmicroscopy AT carolagregor srphiratiometricphbiosensorsforsuperresolutionmicroscopy AT franziskarwinter srphiratiometricphbiosensorsforsuperresolutionmicroscopy AT nicolaiturban srphiratiometricphbiosensorsforsuperresolutionmicroscopy AT steffenjsahl srphiratiometricphbiosensorsforsuperresolutionmicroscopy AT katriniwillig srphiratiometricphbiosensorsforsuperresolutionmicroscopy AT stefanwhell srphiratiometricphbiosensorsforsuperresolutionmicroscopy
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SRpHi ratiometric pH biosensors for super-resolution microscopy

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