Construction of improved tools for protein localization studies in Streptococcus pneumoniae.
We have constructed a set of plasmids that allow efficient expression of both N- and C-terminal fusions of proteins of interest to fluorescent proteins mCherry, Citrine, CFP and GFP in the Gram-positive pathogen Streptococcus pneumoniae. In order to improve expression of the fluorescent fusions to l...
Guardado en:
Autores principales: | Mafalda X Henriques, Maria João Catalão, Joana Figueiredo, João Paulo Gomes, Sergio R Filipe |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Public Library of Science (PLoS)
2013
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Materias: | |
Acceso en línea: | https://doaj.org/article/67e49e3fb30348019514056328010134 |
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