Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders

ABSTRACT: Changes in the metabolic fingerprint of plasma during the onset of lay in broiler breeders were investigated. We used metabolomics to identify biomarkers of sexual maturity and to provide a comprehensive understanding of breeder metabolome during the pullet to hen transition period. A tota...

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Autores principales:	Mohammad Afrouziyeh, Nicole M. Zukiwsky, Douglas R. Korver, Martin J. Zuidhof
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	parent stock sexual development metabolomics metabolic status maturation Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/67eaf2bb4eaf47fb894e91e45e7697c7
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spelling	oai:doaj.org-article:67eaf2bb4eaf47fb894e91e45e7697c72021-11-24T04:23:06ZPlasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders0032-579110.1016/j.psj.2021.101532https://doaj.org/article/67eaf2bb4eaf47fb894e91e45e7697c72022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S003257912100554Xhttps://doaj.org/toc/0032-5791ABSTRACT: Changes in the metabolic fingerprint of plasma during the onset of lay in broiler breeders were investigated. We used metabolomics to identify biomarkers of sexual maturity and to provide a comprehensive understanding of breeder metabolome during the pullet to hen transition period. A total of 36 pullets were used, in which 30 pullets were randomly assigned to one of 10 unique growth trajectories and 6 birds were assigned to an unrestricted group. The growth trajectories were designed using a 3-phase Gompertz growth model with 10 levels of BW gain in the prepubertal and pubertal growth phases ranging from the breeder-recommended target BW to 22.5% higher, in 2.5% increments. The BW trajectories were applied to each individual bird using a precision feeding (PF) system, which collected BW and feed intake data for each individual bird. The birds were classified based on age at first egg (AFE), and 12 pullets were chosen from the lower and upper AFE extremes (early and late onset of lay) at 18, 20, 22, 24, and 26 wk of age to run repeated blood plasma metabolomic assays. The metabolomic profile data were collected using a direct-injection liquid chromatography-tandem mass spectrometry and steroid assays. Univariate analysis identified 87 differential metabolites between the early- and late-onset of lay groups at 24 wk of age and 104 differential metabolites between the pullet and hen groups. Further investigation of differential metabolites showed 15 potential biomarkers for pullet to hen transition by analyzing the receiver operating characteristic (ROC) curve, mainly consisting of carnitine and choline metabolites. Differential metabolites during the pullet to hen transition were mainly associated with lipid, energy, and amino acid metabolism pathways, which gave clues to the physiological and metabolic shifts resulting from sexual maturation. At 24 wk of age, the main pathways involved in differentiation of the early- and late-onset of lay groups were related to lipid and amino acid metabolism. These metabolites could be involved in biosynthesis of egg yolk precursors in the liver.Mohammad AfrouziyehNicole M. ZukiwskyDouglas R. KorverMartin J. ZuidhofElsevierarticleparent stocksexual developmentmetabolomicsmetabolic statusmaturationAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101532- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	parent stock sexual development metabolomics metabolic status maturation Animal culture SF1-1100
spellingShingle	parent stock sexual development metabolomics metabolic status maturation Animal culture SF1-1100 Mohammad Afrouziyeh Nicole M. Zukiwsky Douglas R. Korver Martin J. Zuidhof Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
description	ABSTRACT: Changes in the metabolic fingerprint of plasma during the onset of lay in broiler breeders were investigated. We used metabolomics to identify biomarkers of sexual maturity and to provide a comprehensive understanding of breeder metabolome during the pullet to hen transition period. A total of 36 pullets were used, in which 30 pullets were randomly assigned to one of 10 unique growth trajectories and 6 birds were assigned to an unrestricted group. The growth trajectories were designed using a 3-phase Gompertz growth model with 10 levels of BW gain in the prepubertal and pubertal growth phases ranging from the breeder-recommended target BW to 22.5% higher, in 2.5% increments. The BW trajectories were applied to each individual bird using a precision feeding (PF) system, which collected BW and feed intake data for each individual bird. The birds were classified based on age at first egg (AFE), and 12 pullets were chosen from the lower and upper AFE extremes (early and late onset of lay) at 18, 20, 22, 24, and 26 wk of age to run repeated blood plasma metabolomic assays. The metabolomic profile data were collected using a direct-injection liquid chromatography-tandem mass spectrometry and steroid assays. Univariate analysis identified 87 differential metabolites between the early- and late-onset of lay groups at 24 wk of age and 104 differential metabolites between the pullet and hen groups. Further investigation of differential metabolites showed 15 potential biomarkers for pullet to hen transition by analyzing the receiver operating characteristic (ROC) curve, mainly consisting of carnitine and choline metabolites. Differential metabolites during the pullet to hen transition were mainly associated with lipid, energy, and amino acid metabolism pathways, which gave clues to the physiological and metabolic shifts resulting from sexual maturation. At 24 wk of age, the main pathways involved in differentiation of the early- and late-onset of lay groups were related to lipid and amino acid metabolism. These metabolites could be involved in biosynthesis of egg yolk precursors in the liver.
format	article
author	Mohammad Afrouziyeh Nicole M. Zukiwsky Douglas R. Korver Martin J. Zuidhof
author_facet	Mohammad Afrouziyeh Nicole M. Zukiwsky Douglas R. Korver Martin J. Zuidhof
author_sort	Mohammad Afrouziyeh
title	Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
title_short	Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
title_full	Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
title_fullStr	Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
title_full_unstemmed	Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
title_sort	plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/67eaf2bb4eaf47fb894e91e45e7697c7
work_keys_str_mv	AT mohammadafrouziyeh plasmametabolomicprofilingrevealspotentialonsetoflaybiomarkersinbroilerbreeders AT nicolemzukiwsky plasmametabolomicprofilingrevealspotentialonsetoflaybiomarkersinbroilerbreeders AT douglasrkorver plasmametabolomicprofilingrevealspotentialonsetoflaybiomarkersinbroilerbreeders AT martinjzuidhof plasmametabolomicprofilingrevealspotentialonsetoflaybiomarkersinbroilerbreeders
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Plasma metabolomic profiling reveals potential onset of lay biomarkers in broiler breeders

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