Drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD22-like genes

Abstract Increased biosynthesis of abscisic acid (ABA) occurs in plants in response to water deficit, which is mediated by changes in the levels of reactive oxygen species such as H2O2. Water deficit and ABA induce expression of some RD22-like proteins. This study aimed to evaluate the effect of wat...

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Autores principales:	Kyle Phillips, Ndiko Ludidi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/67eb6c06721646c19268a611cb08d6c4
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spelling	oai:doaj.org-article:67eb6c06721646c19268a611cb08d6c42021-12-02T11:40:45ZDrought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD22-like genes10.1038/s41598-017-08976-x2045-2322https://doaj.org/article/67eb6c06721646c19268a611cb08d6c42017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08976-xhttps://doaj.org/toc/2045-2322Abstract Increased biosynthesis of abscisic acid (ABA) occurs in plants in response to water deficit, which is mediated by changes in the levels of reactive oxygen species such as H2O2. Water deficit and ABA induce expression of some RD22-like proteins. This study aimed to evaluate the effect of water deficit and exogenous ABA (50 µM ABA applied every 24 hours for a total of 72 hours) on H2O2 content in Zea mays (maize) and to characterise genes encoding two putative maize RD22-like proteins (designated ZmRD22A and ZmRD22B). The expression profiles of the two putative maize RD22-like genes in response to water deficit and treatment with ABA were examined in leaves. In silico analyses showed that the maize RD22-like proteins share domain organisation with previously characterized RD22-like proteins. Both water deficit and exogenous ABA resulted in increased H2O2 content in leaves but the increase was more pronounced in response to water deficit than to exogenous ABA. Lignin content was not affected by exogenous ABA, whereas it was decreased by water deficit. Expression of both RD22-like genes was up-regulated by drought but the ZmRD22A gene was not influenced by exogenous ABA, whereas ZmRD22B was highly responsive to exogenous ABA.Kyle PhillipsNdiko LudidiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kyle Phillips Ndiko Ludidi Drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD22-like genes
description	Abstract Increased biosynthesis of abscisic acid (ABA) occurs in plants in response to water deficit, which is mediated by changes in the levels of reactive oxygen species such as H2O2. Water deficit and ABA induce expression of some RD22-like proteins. This study aimed to evaluate the effect of water deficit and exogenous ABA (50 µM ABA applied every 24 hours for a total of 72 hours) on H2O2 content in Zea mays (maize) and to characterise genes encoding two putative maize RD22-like proteins (designated ZmRD22A and ZmRD22B). The expression profiles of the two putative maize RD22-like genes in response to water deficit and treatment with ABA were examined in leaves. In silico analyses showed that the maize RD22-like proteins share domain organisation with previously characterized RD22-like proteins. Both water deficit and exogenous ABA resulted in increased H2O2 content in leaves but the increase was more pronounced in response to water deficit than to exogenous ABA. Lignin content was not affected by exogenous ABA, whereas it was decreased by water deficit. Expression of both RD22-like genes was up-regulated by drought but the ZmRD22A gene was not influenced by exogenous ABA, whereas ZmRD22B was highly responsive to exogenous ABA.
format	article
author	Kyle Phillips Ndiko Ludidi
author_facet	Kyle Phillips Ndiko Ludidi
author_sort	Kyle Phillips
title	Drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD22-like genes
title_short	Drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD22-like genes
title_full	Drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD22-like genes
title_fullStr	Drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD22-like genes
title_full_unstemmed	Drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD22-like genes
title_sort	drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize rd22-like genes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/67eb6c06721646c19268a611cb08d6c4
work_keys_str_mv	AT kylephillips droughtandexogenousabscisicacidalterhydrogenperoxideaccumulationanddifferentiallyregulatetheexpressionoftwomaizerd22likegenes AT ndikoludidi droughtandexogenousabscisicacidalterhydrogenperoxideaccumulationanddifferentiallyregulatetheexpressionoftwomaizerd22likegenes
_version_	1718395519864143872

Drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD22-like genes

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