A Population-Based Surveillance Study of Shared Genotypes of <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from Retail Meat and Suspected Cases of Urinary Tract Infections

ABSTRACT There is increasing evidence that retail food may serve as a source of Escherichia coli that causes community-acquired urinary tract infections, but the impact of this source in a community is not known. We conducted a prospective, population-based study in one community to examine the freq...

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Autores principales:	Reina Yamaji, Cindy R. Friedman, Julia Rubin, Joy Suh, Erika Thys, Patrick McDermott, Melody Hung-Fan, Lee W. Riley
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Escherichia coli extraintestinal diseases molecular epidemiology multidrug resistance multilocus sequence typing urinary tract infection Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/67eeb026aed34d509f358ed16be80c18
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spelling	oai:doaj.org-article:67eeb026aed34d509f358ed16be80c182021-11-15T15:25:50ZA Population-Based Surveillance Study of Shared Genotypes of <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from Retail Meat and Suspected Cases of Urinary Tract Infections10.1128/mSphere.00179-182379-5042https://doaj.org/article/67eeb026aed34d509f358ed16be80c182018-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00179-18https://doaj.org/toc/2379-5042ABSTRACT There is increasing evidence that retail food may serve as a source of Escherichia coli that causes community-acquired urinary tract infections, but the impact of this source in a community is not known. We conducted a prospective, population-based study in one community to examine the frequency of recovery of uropathogenic E. coli genotypes from retail meat samples. We analyzed E. coli isolates from consecutively collected urine samples of patients suspected to have urinary tract infections (UTIs) at a university-affiliated health service and retail meat samples from the same geographic region. We genotyped all E. coli isolates by multilocus sequence typing (MLST) and tested them for antimicrobial susceptibility. From 2016 to 2017, we cultured 233 E. coli isolates from 230 (21%) of 1,087 urine samples and 177 E. coli isolates from 120 (28%) of 427 retail meat samples. Urine samples contained 61 sequence types (STs), and meat samples had 95 STs; 12 STs (ST10, ST38, ST69, ST80, ST88, ST101, ST117, ST131, ST569, ST906, ST1844, and ST2562) were common to both. Thirty-five (81%) of 43 meat isolates among the 12 STs were from poultry. Among 94 isolates in the 12 STs, 26 (60%) of 43 retail meat isolates and 15 (29%) of 51 human isolates were pan-susceptible (P < 0.005). We found that 21% of E. coli isolates from suspected cases of UTIs belonged to STs found in poultry. Poultry may serve as a possible reservoir of uropathogenic E. coli (UPEC). Additional studies are needed to demonstrate transmission pathways of these UPEC genotypes and their food sources. IMPORTANCE Community-acquired urinary tract infection caused by Escherichia coli is one of the most common infectious diseases in the United States, affecting approximately seven million women and costing approximately 11.6 billion dollars annually. In addition, antibiotic resistance among E. coli bacteria causing urinary tract infection continues to increase, which greatly complicates treatment. Identifying sources of uropathogenic E. coli and implementing prevention measures are essential. However, the reservoirs of uropathogenic E. coli have not been well defined. This study demonstrated that poultry sold in retail stores may serve as one possible source of uropathogenic E. coli. This finding adds to a growing body of evidence that suggests that urinary tract infection may be a food-borne disease. More research in this area can lead to the development of preventive strategies to control this common and costly infectious disease.Reina YamajiCindy R. FriedmanJulia RubinJoy SuhErika ThysPatrick McDermottMelody Hung-FanLee W. RileyAmerican Society for MicrobiologyarticleEscherichia coliextraintestinal diseasesmolecular epidemiologymultidrug resistancemultilocus sequence typingurinary tract infectionMicrobiologyQR1-502ENmSphere, Vol 3, Iss 4 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli extraintestinal diseases molecular epidemiology multidrug resistance multilocus sequence typing urinary tract infection Microbiology QR1-502
spellingShingle	Escherichia coli extraintestinal diseases molecular epidemiology multidrug resistance multilocus sequence typing urinary tract infection Microbiology QR1-502 Reina Yamaji Cindy R. Friedman Julia Rubin Joy Suh Erika Thys Patrick McDermott Melody Hung-Fan Lee W. Riley A Population-Based Surveillance Study of Shared Genotypes of <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from Retail Meat and Suspected Cases of Urinary Tract Infections
description	ABSTRACT There is increasing evidence that retail food may serve as a source of Escherichia coli that causes community-acquired urinary tract infections, but the impact of this source in a community is not known. We conducted a prospective, population-based study in one community to examine the frequency of recovery of uropathogenic E. coli genotypes from retail meat samples. We analyzed E. coli isolates from consecutively collected urine samples of patients suspected to have urinary tract infections (UTIs) at a university-affiliated health service and retail meat samples from the same geographic region. We genotyped all E. coli isolates by multilocus sequence typing (MLST) and tested them for antimicrobial susceptibility. From 2016 to 2017, we cultured 233 E. coli isolates from 230 (21%) of 1,087 urine samples and 177 E. coli isolates from 120 (28%) of 427 retail meat samples. Urine samples contained 61 sequence types (STs), and meat samples had 95 STs; 12 STs (ST10, ST38, ST69, ST80, ST88, ST101, ST117, ST131, ST569, ST906, ST1844, and ST2562) were common to both. Thirty-five (81%) of 43 meat isolates among the 12 STs were from poultry. Among 94 isolates in the 12 STs, 26 (60%) of 43 retail meat isolates and 15 (29%) of 51 human isolates were pan-susceptible (P < 0.005). We found that 21% of E. coli isolates from suspected cases of UTIs belonged to STs found in poultry. Poultry may serve as a possible reservoir of uropathogenic E. coli (UPEC). Additional studies are needed to demonstrate transmission pathways of these UPEC genotypes and their food sources. IMPORTANCE Community-acquired urinary tract infection caused by Escherichia coli is one of the most common infectious diseases in the United States, affecting approximately seven million women and costing approximately 11.6 billion dollars annually. In addition, antibiotic resistance among E. coli bacteria causing urinary tract infection continues to increase, which greatly complicates treatment. Identifying sources of uropathogenic E. coli and implementing prevention measures are essential. However, the reservoirs of uropathogenic E. coli have not been well defined. This study demonstrated that poultry sold in retail stores may serve as one possible source of uropathogenic E. coli. This finding adds to a growing body of evidence that suggests that urinary tract infection may be a food-borne disease. More research in this area can lead to the development of preventive strategies to control this common and costly infectious disease.
format	article
author	Reina Yamaji Cindy R. Friedman Julia Rubin Joy Suh Erika Thys Patrick McDermott Melody Hung-Fan Lee W. Riley
author_facet	Reina Yamaji Cindy R. Friedman Julia Rubin Joy Suh Erika Thys Patrick McDermott Melody Hung-Fan Lee W. Riley
author_sort	Reina Yamaji
title	A Population-Based Surveillance Study of Shared Genotypes of <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from Retail Meat and Suspected Cases of Urinary Tract Infections
title_short	A Population-Based Surveillance Study of Shared Genotypes of <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from Retail Meat and Suspected Cases of Urinary Tract Infections
title_full	A Population-Based Surveillance Study of Shared Genotypes of <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from Retail Meat and Suspected Cases of Urinary Tract Infections
title_fullStr	A Population-Based Surveillance Study of Shared Genotypes of <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from Retail Meat and Suspected Cases of Urinary Tract Infections
title_full_unstemmed	A Population-Based Surveillance Study of Shared Genotypes of <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from Retail Meat and Suspected Cases of Urinary Tract Infections
title_sort	population-based surveillance study of shared genotypes of <named-content content-type="genus-species">escherichia coli</named-content> isolates from retail meat and suspected cases of urinary tract infections
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/67eeb026aed34d509f358ed16be80c18
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A Population-Based Surveillance Study of Shared Genotypes of <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from Retail Meat and Suspected Cases of Urinary Tract Infections

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