Mass spectrometry imaging of mice brain lipid profile changes over time under high fat diet

Abstract Overweight and obesity have been shown to significantly affect brain structures and size. Obesity has been associated with cerebral atrophy, alteration of brain functions, including cognitive impairement, and psychiatric diseases such as depression. Given the importance of lipids in the str...

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Autores principales:	Gianluca Sighinolfi, Samantha Clark, Landry Blanc, Daniela Cota, Boutayna Rhourri-Frih
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/67f6cb149d3e40adb87575a4fc368aa4
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spelling	oai:doaj.org-article:67f6cb149d3e40adb87575a4fc368aa42021-12-02T18:01:48ZMass spectrometry imaging of mice brain lipid profile changes over time under high fat diet10.1038/s41598-021-97201-x2045-2322https://doaj.org/article/67f6cb149d3e40adb87575a4fc368aa42021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97201-xhttps://doaj.org/toc/2045-2322Abstract Overweight and obesity have been shown to significantly affect brain structures and size. Obesity has been associated with cerebral atrophy, alteration of brain functions, including cognitive impairement, and psychiatric diseases such as depression. Given the importance of lipids in the structure of the brain, here, by using 47 mice fed a high fat diet (HFD) with 60% calories from fat (40% saturated fatty acids) and 20% calories from carbohydrates and age-matched control animals on a normal chow diet, we examined the effects of HFD and diet-induced obesity on the brain lipidome. Using a targeted liquid chromatography mass spectrometry analysis and a non-targeted mass spectrometry MALDI imaging approach, we show that the relative concentration of most lipids, in particular brain phospholipids, is modified by diet-induced obesity (+ 40%of body weight). Use of a non-targeted MALDI-MS imaging approach further allowed define cerebral regions of interest (ROI) involved in eating behavior and changes in their lipid profile. Principal component analysis (PCA) of the obese/chow lipidome revealed persistence of some of the changes in the brain lipidome of obese animals even after their switch to chow feeding and associated weight loss. Altogether, these data reveal that HFD feeding rapidly modifies the murine brain lipidome. Some of these HFD-induced changes persist even after weight loss, implying that some brain sequelae caused by diet-induced obesity are irreversible.Gianluca SighinolfiSamantha ClarkLandry BlancDaniela CotaBoutayna Rhourri-FrihNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gianluca Sighinolfi Samantha Clark Landry Blanc Daniela Cota Boutayna Rhourri-Frih Mass spectrometry imaging of mice brain lipid profile changes over time under high fat diet
description	Abstract Overweight and obesity have been shown to significantly affect brain structures and size. Obesity has been associated with cerebral atrophy, alteration of brain functions, including cognitive impairement, and psychiatric diseases such as depression. Given the importance of lipids in the structure of the brain, here, by using 47 mice fed a high fat diet (HFD) with 60% calories from fat (40% saturated fatty acids) and 20% calories from carbohydrates and age-matched control animals on a normal chow diet, we examined the effects of HFD and diet-induced obesity on the brain lipidome. Using a targeted liquid chromatography mass spectrometry analysis and a non-targeted mass spectrometry MALDI imaging approach, we show that the relative concentration of most lipids, in particular brain phospholipids, is modified by diet-induced obesity (+ 40%of body weight). Use of a non-targeted MALDI-MS imaging approach further allowed define cerebral regions of interest (ROI) involved in eating behavior and changes in their lipid profile. Principal component analysis (PCA) of the obese/chow lipidome revealed persistence of some of the changes in the brain lipidome of obese animals even after their switch to chow feeding and associated weight loss. Altogether, these data reveal that HFD feeding rapidly modifies the murine brain lipidome. Some of these HFD-induced changes persist even after weight loss, implying that some brain sequelae caused by diet-induced obesity are irreversible.
format	article
author	Gianluca Sighinolfi Samantha Clark Landry Blanc Daniela Cota Boutayna Rhourri-Frih
author_facet	Gianluca Sighinolfi Samantha Clark Landry Blanc Daniela Cota Boutayna Rhourri-Frih
author_sort	Gianluca Sighinolfi
title	Mass spectrometry imaging of mice brain lipid profile changes over time under high fat diet
title_short	Mass spectrometry imaging of mice brain lipid profile changes over time under high fat diet
title_full	Mass spectrometry imaging of mice brain lipid profile changes over time under high fat diet
title_fullStr	Mass spectrometry imaging of mice brain lipid profile changes over time under high fat diet
title_full_unstemmed	Mass spectrometry imaging of mice brain lipid profile changes over time under high fat diet
title_sort	mass spectrometry imaging of mice brain lipid profile changes over time under high fat diet
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/67f6cb149d3e40adb87575a4fc368aa4
work_keys_str_mv	AT gianlucasighinolfi massspectrometryimagingofmicebrainlipidprofilechangesovertimeunderhighfatdiet AT samanthaclark massspectrometryimagingofmicebrainlipidprofilechangesovertimeunderhighfatdiet AT landryblanc massspectrometryimagingofmicebrainlipidprofilechangesovertimeunderhighfatdiet AT danielacota massspectrometryimagingofmicebrainlipidprofilechangesovertimeunderhighfatdiet AT boutaynarhourrifrih massspectrometryimagingofmicebrainlipidprofilechangesovertimeunderhighfatdiet
_version_	1718378930775261184

Mass spectrometry imaging of mice brain lipid profile changes over time under high fat diet

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