Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs

Mutations to SpCas9 can improve fidelity and mitigate off-target effects. Here the authors generate ‘Blackjack’ mutations that improve fidelity while retaining effectiveness with 21G-sgRNAs.

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Autores principales:	Péter István Kulcsár, András Tálas, Eszter Tóth, Antal Nyeste, Zoltán Ligeti, Zsombor Welker, Ervin Welker
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/67fc5782aecd46679b8ed3c69f01ff88
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spelling	oai:doaj.org-article:67fc5782aecd46679b8ed3c69f01ff882021-12-02T15:39:24ZBlackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs10.1038/s41467-020-15021-52041-1723https://doaj.org/article/67fc5782aecd46679b8ed3c69f01ff882020-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15021-5https://doaj.org/toc/2041-1723Mutations to SpCas9 can improve fidelity and mitigate off-target effects. Here the authors generate ‘Blackjack’ mutations that improve fidelity while retaining effectiveness with 21G-sgRNAs.Péter István KulcsárAndrás TálasEszter TóthAntal NyesteZoltán LigetiZsombor WelkerErvin WelkerNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Péter István Kulcsár András Tálas Eszter Tóth Antal Nyeste Zoltán Ligeti Zsombor Welker Ervin Welker Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs
description	Mutations to SpCas9 can improve fidelity and mitigate off-target effects. Here the authors generate ‘Blackjack’ mutations that improve fidelity while retaining effectiveness with 21G-sgRNAs.
format	article
author	Péter István Kulcsár András Tálas Eszter Tóth Antal Nyeste Zoltán Ligeti Zsombor Welker Ervin Welker
author_facet	Péter István Kulcsár András Tálas Eszter Tóth Antal Nyeste Zoltán Ligeti Zsombor Welker Ervin Welker
author_sort	Péter István Kulcsár
title	Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs
title_short	Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs
title_full	Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs
title_fullStr	Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs
title_full_unstemmed	Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs
title_sort	blackjack mutations improve the on-target activities of increased fidelity variants of spcas9 with 5′g-extended sgrnas
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/67fc5782aecd46679b8ed3c69f01ff88
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Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs

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