Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs
Mutations to SpCas9 can improve fidelity and mitigate off-target effects. Here the authors generate ‘Blackjack’ mutations that improve fidelity while retaining effectiveness with 21G-sgRNAs.
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Auteurs principaux: | Péter István Kulcsár, András Tálas, Eszter Tóth, Antal Nyeste, Zoltán Ligeti, Zsombor Welker, Ervin Welker |
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Format: | article |
Langue: | EN |
Publié: |
Nature Portfolio
2020
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Sujets: | |
Accès en ligne: | https://doaj.org/article/67fc5782aecd46679b8ed3c69f01ff88 |
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