A Metabolic Profiling Analysis Revealed a Primary Metabolism Reprogramming in Arabidopsis <i>glyI4</i> Loss-of-Function Mutant

Methylglyoxal (MG) is a cytotoxic compound often produced as a side product of metabolic processes such as glycolysis, lipid peroxidation, and photosynthesis. MG is mainly scavenged by the glyoxalase system, a two-step pathway, in which the coordinate activity of GLYI and GLYII transforms it into D-...

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Autores principales:	Silvia Proietti, Laura Bertini, Gaia Salvatore Falconieri, Ivan Baccelli, Anna Maria Timperio, Carla Caruso
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	methylglyoxal glyoxalase I metabolite profiling oxidative stress plant growth plant defense Botany QK1-989
Acceso en línea:	https://doaj.org/article/68053fa1bd694209a4e57a0d607f9588
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spelling	oai:doaj.org-article:68053fa1bd694209a4e57a0d607f95882021-11-25T18:46:48ZA Metabolic Profiling Analysis Revealed a Primary Metabolism Reprogramming in Arabidopsis <i>glyI4</i> Loss-of-Function Mutant10.3390/plants101124642223-7747https://doaj.org/article/68053fa1bd694209a4e57a0d607f95882021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2464https://doaj.org/toc/2223-7747Methylglyoxal (MG) is a cytotoxic compound often produced as a side product of metabolic processes such as glycolysis, lipid peroxidation, and photosynthesis. MG is mainly scavenged by the glyoxalase system, a two-step pathway, in which the coordinate activity of GLYI and GLYII transforms it into D-lactate, releasing GSH. In <i>Arabidopsis thaliana</i>, a member of the GLYI family named GLYI4 has been recently characterized. In <i>glyI4</i> mutant plants, a general stress phenotype characterized by compromised MG scavenging, accumulation of reactive oxygen species (ROS), stomatal closure, and reduced fitness was observed. In order to shed some light on the impact of <i>gly4</i> loss-of-function on plant metabolism, we applied a high resolution mass spectrometry-based metabolomic approach to Arabidopsis Col-8 wild type and <i>glyI4</i> mutant plants. A compound library containing a total of 70 metabolites, differentially synthesized in <i>glyI4</i> compared to Col-8, was obtained. Pathway analysis of the identified compounds showed that the upregulated pathways are mainly involved in redox reactions and cellular energy maintenance, and those downregulated in plant defense and growth. These results improved our understanding of the impacts of <i>glyI4</i> loss-of-function on the general reprogramming of the plant’s metabolic landscape as a strategy for surviving under adverse physiological conditions.Silvia ProiettiLaura BertiniGaia Salvatore FalconieriIvan BaccelliAnna Maria TimperioCarla CarusoMDPI AGarticlemethylglyoxalglyoxalase Imetabolite profilingoxidative stressplant growthplant defenseBotanyQK1-989ENPlants, Vol 10, Iss 2464, p 2464 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	methylglyoxal glyoxalase I metabolite profiling oxidative stress plant growth plant defense Botany QK1-989
spellingShingle	methylglyoxal glyoxalase I metabolite profiling oxidative stress plant growth plant defense Botany QK1-989 Silvia Proietti Laura Bertini Gaia Salvatore Falconieri Ivan Baccelli Anna Maria Timperio Carla Caruso A Metabolic Profiling Analysis Revealed a Primary Metabolism Reprogramming in Arabidopsis <i>glyI4</i> Loss-of-Function Mutant
description	Methylglyoxal (MG) is a cytotoxic compound often produced as a side product of metabolic processes such as glycolysis, lipid peroxidation, and photosynthesis. MG is mainly scavenged by the glyoxalase system, a two-step pathway, in which the coordinate activity of GLYI and GLYII transforms it into D-lactate, releasing GSH. In <i>Arabidopsis thaliana</i>, a member of the GLYI family named GLYI4 has been recently characterized. In <i>glyI4</i> mutant plants, a general stress phenotype characterized by compromised MG scavenging, accumulation of reactive oxygen species (ROS), stomatal closure, and reduced fitness was observed. In order to shed some light on the impact of <i>gly4</i> loss-of-function on plant metabolism, we applied a high resolution mass spectrometry-based metabolomic approach to Arabidopsis Col-8 wild type and <i>glyI4</i> mutant plants. A compound library containing a total of 70 metabolites, differentially synthesized in <i>glyI4</i> compared to Col-8, was obtained. Pathway analysis of the identified compounds showed that the upregulated pathways are mainly involved in redox reactions and cellular energy maintenance, and those downregulated in plant defense and growth. These results improved our understanding of the impacts of <i>glyI4</i> loss-of-function on the general reprogramming of the plant’s metabolic landscape as a strategy for surviving under adverse physiological conditions.
format	article
author	Silvia Proietti Laura Bertini Gaia Salvatore Falconieri Ivan Baccelli Anna Maria Timperio Carla Caruso
author_facet	Silvia Proietti Laura Bertini Gaia Salvatore Falconieri Ivan Baccelli Anna Maria Timperio Carla Caruso
author_sort	Silvia Proietti
title	A Metabolic Profiling Analysis Revealed a Primary Metabolism Reprogramming in Arabidopsis <i>glyI4</i> Loss-of-Function Mutant
title_short	A Metabolic Profiling Analysis Revealed a Primary Metabolism Reprogramming in Arabidopsis <i>glyI4</i> Loss-of-Function Mutant
title_full	A Metabolic Profiling Analysis Revealed a Primary Metabolism Reprogramming in Arabidopsis <i>glyI4</i> Loss-of-Function Mutant
title_fullStr	A Metabolic Profiling Analysis Revealed a Primary Metabolism Reprogramming in Arabidopsis <i>glyI4</i> Loss-of-Function Mutant
title_full_unstemmed	A Metabolic Profiling Analysis Revealed a Primary Metabolism Reprogramming in Arabidopsis <i>glyI4</i> Loss-of-Function Mutant
title_sort	metabolic profiling analysis revealed a primary metabolism reprogramming in arabidopsis <i>glyi4</i> loss-of-function mutant
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/68053fa1bd694209a4e57a0d607f9588
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A Metabolic Profiling Analysis Revealed a Primary Metabolism Reprogramming in Arabidopsis <i>glyI4</i> Loss-of-Function Mutant

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