Comparison of MSC-Neurogenin1 administration modality in MCAO rat model

Intracerebral (IC) grafting of mesenchymal stem cells (MSCs) is not currently used in humans due to its potential complications. On the other hand, intra-arterial (IA) administration can be facilitated for engrafting of intensifed MSCs in the injured human brain. The study is designed to compare the...

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Autores principales:	Shin Dong Hoon, Kim Gyu-Hee, Lee Jin Soo, Joo In Soo, Suh-Kim Haeyoung, Kim Sung-Soo, Hong Ji Man
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2016
Materias:	brain ischemia cell transplantation mesenchymal stem cells Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/680640e09ffd467bac3f81c07ea50436
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spelling	oai:doaj.org-article:680640e09ffd467bac3f81c07ea504362021-12-05T14:11:04ZComparison of MSC-Neurogenin1 administration modality in MCAO rat model2081-693610.1515/tnsci-2016-0024https://doaj.org/article/680640e09ffd467bac3f81c07ea504362016-12-01T00:00:00Zhttps://doi.org/10.1515/tnsci-2016-0024https://doaj.org/toc/2081-6936Intracerebral (IC) grafting of mesenchymal stem cells (MSCs) is not currently used in humans due to its potential complications. On the other hand, intra-arterial (IA) administration can be facilitated for engrafting of intensifed MSCs in the injured human brain. The study is designed to compare the two methods of MSC administration using IA and IC routes through the parameters of behavior, infarct volume, cell distribution, and MSC identification. An ischemic stroke model was generated in Sprague Dawley male rats. This experiment used MSCs/Ngn1 that express Neurogenin1 (Ngn1) to ensure grafted MSC maintenance. MSCs/Ngn1 or normal saline was administrated via the IC or IA route on day 3. All animals were randomly assigned into four groups (five rats in each group): IC-control, IA-control, IC-MSCs/Ngn1, or IA-MSCs/Ngn1. Motor behaviors, infarct volume, and distribution of superparamagnetic iron oxide (SPIO)-labeled cells on magnetic resonance imaging (MRI) were compared from each group. There were no baseline differencess in motor behaviors or infarct volume between IC-MSCs/Ngn1 and IA-MSCs/Ngn1. Hovever, the IA-MSCs/Ngn1 group showed the greatest recovery on Rotarod testing and adhesive removal tests (p = 0.003 and p = 0.009 vs. IC-MSCs/Ngn1, respectively). The IA-MSCs/Ngn1 group also had more evenly distributed SPIO-labeled cells on MRI. The results suggest that IA administration is likely to be benefcial for humans based on its ability to improve behavioral outcomes and ensure even MSC engrafting.Shin Dong HoonKim Gyu-HeeLee Jin SooJoo In SooSuh-Kim HaeyoungKim Sung-SooHong Ji ManDe Gruyterarticlebrain ischemiacell transplantationmesenchymal stem cellsNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENTranslational Neuroscience, Vol 7, Iss 1, Pp 164-172 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	brain ischemia cell transplantation mesenchymal stem cells Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	brain ischemia cell transplantation mesenchymal stem cells Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Shin Dong Hoon Kim Gyu-Hee Lee Jin Soo Joo In Soo Suh-Kim Haeyoung Kim Sung-Soo Hong Ji Man Comparison of MSC-Neurogenin1 administration modality in MCAO rat model
description	Intracerebral (IC) grafting of mesenchymal stem cells (MSCs) is not currently used in humans due to its potential complications. On the other hand, intra-arterial (IA) administration can be facilitated for engrafting of intensifed MSCs in the injured human brain. The study is designed to compare the two methods of MSC administration using IA and IC routes through the parameters of behavior, infarct volume, cell distribution, and MSC identification. An ischemic stroke model was generated in Sprague Dawley male rats. This experiment used MSCs/Ngn1 that express Neurogenin1 (Ngn1) to ensure grafted MSC maintenance. MSCs/Ngn1 or normal saline was administrated via the IC or IA route on day 3. All animals were randomly assigned into four groups (five rats in each group): IC-control, IA-control, IC-MSCs/Ngn1, or IA-MSCs/Ngn1. Motor behaviors, infarct volume, and distribution of superparamagnetic iron oxide (SPIO)-labeled cells on magnetic resonance imaging (MRI) were compared from each group. There were no baseline differencess in motor behaviors or infarct volume between IC-MSCs/Ngn1 and IA-MSCs/Ngn1. Hovever, the IA-MSCs/Ngn1 group showed the greatest recovery on Rotarod testing and adhesive removal tests (p = 0.003 and p = 0.009 vs. IC-MSCs/Ngn1, respectively). The IA-MSCs/Ngn1 group also had more evenly distributed SPIO-labeled cells on MRI. The results suggest that IA administration is likely to be benefcial for humans based on its ability to improve behavioral outcomes and ensure even MSC engrafting.
format	article
author	Shin Dong Hoon Kim Gyu-Hee Lee Jin Soo Joo In Soo Suh-Kim Haeyoung Kim Sung-Soo Hong Ji Man
author_facet	Shin Dong Hoon Kim Gyu-Hee Lee Jin Soo Joo In Soo Suh-Kim Haeyoung Kim Sung-Soo Hong Ji Man
author_sort	Shin Dong Hoon
title	Comparison of MSC-Neurogenin1 administration modality in MCAO rat model
title_short	Comparison of MSC-Neurogenin1 administration modality in MCAO rat model
title_full	Comparison of MSC-Neurogenin1 administration modality in MCAO rat model
title_fullStr	Comparison of MSC-Neurogenin1 administration modality in MCAO rat model
title_full_unstemmed	Comparison of MSC-Neurogenin1 administration modality in MCAO rat model
title_sort	comparison of msc-neurogenin1 administration modality in mcao rat model
publisher	De Gruyter
publishDate	2016
url	https://doaj.org/article/680640e09ffd467bac3f81c07ea50436
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Comparison of MSC-Neurogenin1 administration modality in MCAO rat model

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